Nitric oxide donors, nitric oxide synthase inhibitors and nitric oxide detection reagents and kits for nitric oxide (NO) studies.

• Nitric oxide donors are available as stand-alone reagents and combination kit
• Nitric oxide synthase inhibitors are available as stand-alone reagents and combination kit
• Nitric oxide detection kit is based on Griess reagent

Nitric Oxide Donors: SIN-1 releases nitric oxide and forms superoxide spontaneously under physiological conditions and has been shown to be a potent vasodilator. SNAP releases nitric oxide under physiological conditions and also been shown to be a potent vasodilator. Similar to SNAP, S-nitrosogluthathione releases nitric oxide spontaneously under physiological conditions. Spermine NONOate releases nitric oxide spontaneously under physiological conditions, and is useful for reliable NO generation in solution. DEA-NONOate releases nitric oxide spontaneously under physiological conditions. Useful for reliable NO generation in solution. The Nitric Oxide Generation Kit contains10 mg each of the following nitric oxide donors: SIN-1, SNAP, S-Nitrosoglutathione, spermine NONOate, and DEA-NONOate.

Nitric Oxide Synthase Inhibitors: L-NMMA competitively inhibits nitric oxide synthase in a wide range of cells. L-NIL is a potent and selective inhibitor of nitric oxide synthase that exhibits about 28-fold greater selectivity for inducible nitric oxide synthase (IC50 = 3,3 µM) than for the rat brain constitutive enzyme (IC50 = 92 µM). L-NIO is a potent, irreversible inhibitor of neutrophil nitric oxide synthase. The NOS Inhibitor kit contains 7-NI, L-NMMA, L-NIL and L-NIO.

Nitric Oxide Detection Kit: Griess reagent is used to detect nitrite photometrically. The reagent contains two chemicals, sulfanilic acid andN-(1-naphthalenediamine). Under acidic conditions sulfanilic acid is converted by nitrite to a diazonium salt, which readily couples with N-(1-naphthalenediamine) to form a highly colored azo dye that can be detected at 548 nm. Under physiological conditionss, NO is unstable and is rapidly oxidized to a mixture of nitrite and nitrate. In order to measure NO levels indirectly from nitrite, nitrate must be reduced to nitrite enzymatically using nitrate reductase (not included) before performing the assay so that the total amount of nitrite can be measured. The kit includes ready-to-use Griess reagent, nitrite standard solution, and a detailed protocol.