Crosslink magnetic IP/Co-IP kit, Pierce™
Catalog # PIER88805
Supplier: Thermo Fisher Scientific
CAS Number:
Specifications
- Pk:1 KIT
- Description:Crosslink Magnetic IP/Co-IP kit, sufficient for 40 IP reactions using 25 μl of beads
Specifications
About this item
The Pierce™ Crosslink Magnetic IP/Co-IP Kit uses crosslinking chemistry to covalently immobilise IP antibodies onto premium-quality protein A/G magnetic beads for effective immunoprecipitation and co-immunoprecipitation.
- Antibody is irreversibly attached to the magnetic beads resulting in negligible co-elution of intact antibody or heavy and light chains with the purified antigen
- Convenient – the kit contains magnetic beads and all essential buffers for optimal immunoprecipitation
- Complete crosslinking and immunoprecipitation in less than three hours
- Immunoprecipitate with half the recommended volume of magnetic particles compared to other magnetic beads
- Magnetic beads coupled with Protein A/G recombinant protein ensures compatibility with most primary antibodies, whether from mouse or rabbit
- Use only the amount of antibody needed for a single IP experiment or prepare a larger quantity of antibody-crosslinked magnetic beads for multiple experiments
Delivery: The kit contains protein A/G magnetic beads, 1 ml; IP lysis/wash buffer, 2×50 ml; coupling buffer (20X), 25 ml; DSS crosslinker, 8×2 mg; lane marker sample buffer (5X), 5 ml; elution buffer, 25 ml; neutralisation buffer, 1 ml; and lane marker sample buffer (5X), 5 ml