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Crosslink magnetic IP/Co-IP kit, Pierce™
Crosslink magnetic IP/Co-IP kit, Pierce™
Catalog # PIER88805
CAS Number:  
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Crosslink magnetic IP/Co-IP kit, Pierce™
Catalog # PIER88805
CAS Number:  

Specifications

  • Pk:
    1 KIT
  • Description:
    Crosslink Magnetic IP/Co-IP kit, sufficient for 40 IP reactions using 25 μl of beads

Specifications

About this item

The Pierce™ Crosslink Magnetic IP/Co-IP Kit uses crosslinking chemistry to covalently immobilise IP antibodies onto premium-quality protein A/G magnetic beads for effective immunoprecipitation and co-immunoprecipitation.

  • Antibody is irreversibly attached to the magnetic beads resulting in negligible co-elution of intact antibody or heavy and light chains with the purified antigen
  • Convenient – the kit contains magnetic beads and all essential buffers for optimal immunoprecipitation
  • Complete crosslinking and immunoprecipitation in less than three hours
  • Immunoprecipitate with half the recommended volume of magnetic particles compared to other magnetic beads
  • Magnetic beads coupled with Protein A/G recombinant protein ensures compatibility with most primary antibodies, whether from mouse or rabbit
  • Use only the amount of antibody needed for a single IP experiment or prepare a larger quantity of antibody-crosslinked magnetic beads for multiple experiments
Delivery: The kit contains protein A/G magnetic beads, 1 ml; IP lysis/wash buffer, 2×50 ml; coupling buffer (20X), 25 ml; DSS crosslinker, 8×2 mg; lane marker sample buffer (5X), 5 ml; elution buffer, 25 ml; neutralisation buffer, 1 ml; and lane marker sample buffer (5X), 5 ml