Subcellular Protein Fractionation Kit for Cultured Cells
78840.
:
- Pk:1 KIT
- Description:Subcellular protein fractionation kit for cultured cells, sufficient for 50 samples each containing 2 million cells (20 µl packed)
This kit is ideal for segregation and enrichment of proteins from five different cellular compartments.
- Concise - extract functional cytoplasmic, membrane, nuclear soluble, chromatin-bound and cytoskeletal protein fractions in less than 3 hours
- Convenient - perform a simple procedure without using ultracentrifugation over gradients
- Complete - obtain protein fractions from a single kit
- Cultured cells - developed and optimized for use with cultured mammalian cells
- Compatible - use extracts for downstream applications such as protein assays, Western blotting, protein assays, electrophoretic mobility shift assays, reporter-gene and enzyme-activity assays
: Kit includes four extraction buffers, a stabilised nuclease and halt protease inhibitor cocktail.