Expression kits for PCR-based generation of linear templates.

RTS Linear Template Kit Plus: For accelerated screening of protein expression templates and conditions
Fast generation of multiple constructs
Efficient in vitro expression (E. coli and insect)
Primers for affinity tags (Strep-tag II/His6-tag)
Signal peptide sequence primers for expression in insects

RTS Linear Template Fab Kit: Template generation Kit for Disulfide E. coli and Insect Kits; PCR based template generation within 1 day for disulfide protein expression
Rapid linear template generation for functional testing of antibody fragments (Fab, scFv) and disulfide bonded proteins
Introduction of an affinity tag for protein purification
Introduction of a signal peptide sequence for translocation into microsomes
Compatible with both Disulfide E. coli and Disulfide Insect Kits

RTS 100 E. coli LinTempGenSet, His-tag: Generate linear templates for rapid screening and optimisation of 6×His-tagged protein expression
Fast template generation without cloning
Contains all regulatory elements in optimal spacing
Create templates with N- or C-terminal 6×His-tag
Primary PCR product can also be cloned into pIVEX vectors
For use with RTS 100 E. coli HY Kit

RTS Wheat Germ LinTempGen Set, His6-tag: Generate linear templates for rapid screening and optimization of 6×His-tagged protein expression
Rapidly create linear expression constructs
Optimised spacing of eukaryotic regulatory elements
Save time; no laborious cloning
Take advantage of flexible positioning of 6×His-tag