The Nessler tubes, with shadowless bottom that transmits undistorted light, are ideal for colour comparison. Rings and markings are in durable white ceramic enamel which correspond with specific volumes of liquid. Graduated ring scale of any of the tubes within a set will not vary from that of any other tube within the set by more than 1,5 mm.

The 50 ml size tube is specifically mentioned by the American Public Health Association in standard methods for the examination of water and waste water. Tubes are manufactured from 33 expansion, low extractable borosilicate glass conforming to USP Type I and ASTM E438, Type I Class A requirements.