The Overnight Express™ Autoinduction Systems enable regulated protein expression in E. coli, without monitoring the culture or adding inducer during cell growth.
- Autoinduction media simplifies protocol by eliminating the monitoring and induction steps
- Rich culture media for high cell densities and protein expression level
- Granulated format allows rapid and uniform dissolution in water and minimises the chance of inhaling hazardous powdered ingredients
- Single use EasyPak packaging reduces sample handling significantly and reduces the chance of contamination
- Convenient, no need to spend extra time on weighing out different ingredients
With Overnight Express™ Autoinduction Systems, a period of cell growth is followed by spontaneous induction of protein expression – without monitoring cell density and without conventional induction with IPTG. The method is based on media components that are metabolised differentially to promote growth to high density and automatically induce protein expression from lac promoters.
The Overnight Express™ Autoinduction System is extremely convenient for routine expression of proteins in multiple cultures and is ideal for high-throughput parallel analysis of protein expression, solubility, and purification from multiple expression clones.
Overnight Express™ Instant LB and TB Media: Granulated Instant LB Medium and TB Medium formulations are combinations of Overnight Express™ System 1 with either Luria-Bertani Broth (LB) or Terrific Broth (TB). These convenient, rich-culture autoinduction media provide high level protein production in pET and other IPTG-inducible bacterial expression systems.
Overnight Express™ Instant LB and Instant TB Medium are ideal for routine expression of proteins in multiple cultures and
for high-throughput parallel analysis of protein expression, solubility and purification from multiple expression clones.