Sephadex G-50 grade F is a size exclusion chromatography resin designed for desalting and buffer exchange, and for purification of DNA and oligonucleotides greater than 20 bases in length using spin columns.

• For purification of DNA from small molecules by size exclusion
• Tested to ensure reproducibly high recovery of DNA
• Sephadex G-50 DNA Grade is used in various G-50 columns, such as AutoSeq G-50 columns, ProbeQuant G-50 Micro Columns, MicroSpin G-50 Columns, and NICK Columns
• Suitable for researchers who prefer to prepare their own columns for purifying nucleic acids
• Excellent for rapid purification of newly synthesized oligonucleotides > 10-mers, including oligonucleotide probes, in 100 to 150 µl of deprotection solution using spin-column chromatography
• Useful for desalting or buffer exchange of DNA and removal of unincorporated radionucleotides from end-labeled oligonucleotides (at least 10 bases in length) in a volume of 10 to 100 µl

Sephadex G-50 is one of five different G-types ranging from G-10 for small molecules to G-75 for larger molecules. Sephadex G-50 is a well-established gel filtration resin for desalting and buffer exchange of biomolecules > 30 000 molecular weight, and with a spin protocol can be used for DNA and oligo purification of molecules greater than 20 bases in length.

Parameters
Particle Size Dry: 50 µm to 150 µm
Application: For purification of DNA from small molecules by gel filtration
Fractionation range (Mr) (globular proteins): 1500 to 30000
Fractionation (Mp) Dextrans: 500 to 10000
Fractionation Range (Mr) DNA Exclusion Limit: 20