PD MidiTrap G-25 is convenient and rapid cleanup with high reproducibility and the potential for high throughput by running multiple samples in parallel, The use of centrifugation protocol with all gravity columns.

• Reliable and reproducible clean-up of proteins and other large biomolecules (>5000 molecular weight)
• Ideal for desalting, buffer exchange, and removal of low molecular weight compounds
• High desalting capacity
• Flexible protocols to use with gravity flow or centrifugation
• Sample preparation in the range of 0,5 to 1,0 ml for gravity and 0,75 to 1,0 ml with centrifugation

PD MidiTrap G-25 is designed for convenient desalting and buffer exchange of biological samples and volumes up to 1.0 ml can be handled. Two alternative application protocols are available, gravity or centrifugation. By using the gravity protocol, a simple cleanup of the sample is done with one or several columns in parallel without any need for a purification system. With the centrifugation protocol, the samples are run in parallel in a standard centrifuge with minimal dilution of the eluted sample.

For larger scale please use PD-10 desalting column (2,5 ml) and for smaller scale, PD MiniTrap™ G-25 (0,5 ml), PD SpinTrap™ G-25 (130 µl) and PD MultiTrap™ G-25 (130 µl) in parallel.