Cells

Supplier: ProSci Inc.

Fetus lysate (7 Day Fetus) was prepared by homogenisation in modified RIPA buffer (150 mM sodium chloride, 50 mM Tris-HCl, pH 7,4, 1 mM ethylenediaminetetraacetic acid, 1 mM phenylmethylsulfonyl fluoride, 1% Triton X-100, 1% sodium deoxycholic acid, 0,1% sodium dodecylsulfate, 5 μg/ml of aprotinin, 5 μg/ml of leupeptin.

Supplier: Novus Biologicals

TMEM173/MPYS Overexpression Lysate (Adult Normal)

Supplier: Charles River Laboratories Cell Solutions

CD4+ Helper T cells are isolated from mononuclear cells by positive selection using immunomagnetic cell separation procedures. Peripheral blood cells are collected from normal human volunteers by direct collection or by using the COBE® Spectra or Spectra Optia® Apheresis System where cell-rich leukapheresis product is extracted using continuous flow centrifugal technology directly into the collection bag.

Supplier: G-Biosciences

GenLysates™ are total protein tissue and cell lysates that are extracted from the tissue or cell of interest. To inhibit proteolysis, the GenLysates™ are prepared in a lysis buffer containing a complete protease inhibitor cocktail. Each lysate lot is tested by electrophoresis and Western blot analysis.

Supplier: G-Biosciences

GenLysates™ are total protein tissue and cell lysates that are extracted from the tissue or cell of interest. To inhibit proteolysis, the GenLysates™ are prepared in a lysis buffer containing a complete protease inhibitor cocktail. Each lysate lot is tested by electrophoresis and Western blot analysis.

Supplier: G-Biosciences

GenLysates™ are total protein tissue and cell lysates that are extracted from the tissue or cell of interest. To inhibit proteolysis, the GenLysates™ are prepared in a lysis buffer containing a complete protease inhibitor cocktail. Each lysate lot is tested by electrophoresis and Western blot analysis.

Supplier: G-Biosciences

GenLysates™ are total protein tissue and cell lysates that are extracted from the tissue or cell of interest. To inhibit proteolysis, the GenLysates™ are prepared in a lysis buffer containing a complete protease inhibitor cocktail. Each lysate lot is tested by electrophoresis and Western blot analysis.

Supplier: PROVITRO

HFIB-g - Human Fibroblast from gingiva - are obtained from human tissue, in particularly from the gingival fibroblasts, initially isolated in their in vivo state. The cells are then transitioned into a primary cell culture and subsequently aliquoted and frozen for preservation. It's crucial to note that HFIB-g cultures undergo careful methods during isolation, ensuring that they remain untransformed and unmutated, maintaining characteristics reflective of their in vivo state. These primary cultures have a limited in-vitro lifespan.

Supplier: Charles River Laboratories Cell Solutions

Human mobilised peripheral blood CD133⁺ stem/progenitor cells are isolated from peripheral blood mononuclear cells (PBMCs) using positive immunomagnetic cell separation procedures. Peripheral blood cells are collected from normal human volunteers by direct collection or by using the COBE® Spectra or Spectra Optia® Apheresis System where cell-rich leukapheresis product is extracted using continuous flow centrifugal technology directly into the collection bag.

Supplier: G-Biosciences

GenLysates™ are total protein tissue and cell lysates that are extracted from the tissue or cell of interest. To inhibit proteolysis, the GenLysates™ are prepared in a lysis buffer containing a complete protease inhibitor cocktail. Each lysate lot is tested by electrophoresis and Western blot analysis.

Supplier: ProSci Inc.

Brain tissue lysate (14 Days Old) was prepared by homogenisation in modified RIPA buffer (150 mM sodium chloride, 50 mM Tris-HCl, pH 7,4, 1 mM ethylenediaminetetraacetic acid, 1 mM phenylmethylsulfonyl fluoride, 1% Triton X-100, 1% sodium deoxycholic acid, 0,1% sodium dodecylsulfate, 5 μg/ml of aprotinin, 5 μg/ml of leupeptin.

Supplier: ProSci Inc.

Fetus lysate (14 Day Fetus) was prepared by homogenisation in modified RIPA buffer (150 mM sodium chloride, 50 mM Tris-HCl, pH 7,4, 1 mM ethylenediaminetetraacetic acid, 1 mM phenylmethylsulfonyl fluoride, 1% Triton X-100, 1% sodium deoxycholic acid, 0,1% sodium dodecylsulfate, 5 μg/ml of aprotinin, 5 μg/ml of leupeptin.

Supplier: PROVITRO

HUAEC - Human Umbilical Artery Endothelial Cells) are obtained from human tissue, initially isolated in their in vivo state. The cells are then transitioned into a primary cell culture and subsequently aliquoted and frozen for preservation. It's crucial to note that HUAEC cultures undergo careful methods during isolation, ensuring that they remain untransformed and unmutated, maintaining characteristics reflective of their in vivo state. These primary cultures have a limited in-vitro lifespan.

Supplier: PROVITRO

HPASMC - Human pulmonary artery smooth muscle cells - are obtained from human tissue, in particularly from the pulmonary artery , initially isolated in their in vivo state. The cells are then transitioned into a primary cell culture and subsequently aliquoted and frozen for preservation. It's crucial to note that HPASMC cultures undergo careful methods during isolation, ensuring that they remain untransformed and unmutated, maintaining characteristics reflective of their in vivo state. These primary cultures have a limited in-vitro lifespan.

Supplier: Charles River Laboratories Cell Solutions

CD34⁺ cells are isolated using positive immunomagnetic cell separation procedures from bone marrow. Bone marrow aspirates and cells, drawn from the posterior iliac crest using a syringe containing anticoagulant, are collected from normal human volunteers using institutional review board (IRB) approved procedures.

Supplier: BIOMOL RESEARCH LABORATORIES

Nuclear extract from HeLa cells is a rich source of HDAC activity which is used for inhibitor screening or immunoprecipitation of HDAC containing complexes.