Cells

Supplier: Charles River Laboratories Cell Solutions

M1 and M 2 macrophages are derived from purified CD14⁺ monocytes. CD14⁺ monocytes were positively selected via immunomagnetic beads and were cultured in the presence of GM-CSF(M1) or M-CSF(M2) for 10 days. The phenotyping was confirmed by morphology and the expression of specific biomarkers.

Supplier: Charles River Laboratories Cell Solutions

CD19⁺ B cells are isolated from mononuclear cells by positive or negative selection using immunomagnetic cell separation procedures. Peripheral blood cells are collected from normal human volunteers by direct collection or by using the COBE® Spectra or Spectra Optia® Apheresis System where cell-rich leukapheresis product is extracted using continuous flow centrifugal technology directly into the collection bag.

Supplier: ProSci Inc.

Ovary tissue lysate was prepared by homogenization in lysis buffer (10 mM HEPES pH 7,9, 1,5 mM MgCl₂, 10 mM KCl, 1 mM ethylenediaminetetraacetic acid, 10% glycerol, 1% NP-40, and a cocktail of protease inhibitors).

Supplier: ProSci Inc.

Skin tissue lysate (14 Days Old) was prepared by homogenisation in modified RIPA buffer (150 mM sodium chloride, 50 mM Tris-HCl, pH 7,4, 1 mM ethylenediaminetetraacetic acid, 1 mM phenylmethylsulfonyl fluoride, 1% Triton X-100, 1% sodium deoxycholic acid, 0,1% sodium dodecylsulfate, 5 μg/ml of aprotinin, 5 μg/ml of leupeptin.

Supplier: Novus Biologicals

p38 Overexpression Lysate (Adult Normal)

Supplier: Charles River Laboratories Cell Solutions

Naive CD4⁺ T cells are isolated from mononuclear cells by positive selection using immunomagnetic cell separation procedures.

Supplier: PROVITRO

CD14⁺ T cells, obtained from mononuclear cells, are isolated through either negative or positive selection using immunogenetic cell separation techniques. These cells play a crucial role in the immune response against infections and are valuable for the investigation and diagnosis of immune reactions, particularly in infections and inflammation.

Supplier: PROVITRO

CD34⁺ stem and progenitor cells from umbilical cord blood are crucial for haematopoiesis and modulation of the immune response. These unique cells contribute to the formation of various blood cells, including immune effectors such as natural killer cells and cytotoxic T-cells, shaping both the adaptive and functional aspects of the immune system.

Supplier: ProSci Inc.

Liver tissue lysate (7 Days Old) was prepared by homogenisation in modified RIPA buffer (150 mM sodium chloride, 50 mM Tris-HCl, pH 7,4, 1 mM ethylenediaminetetraacetic acid, 1 mM phenylmethylsulfonyl fluoride, 1% Triton X-100, 1% sodium deoxycholic acid, 0,1% sodium dodecylsulfate, 5 μg/ml of aprotinin, 5 μg/ml of leupeptin.

Supplier: G-Biosciences

GenLysates™ are total protein tissue and cell lysates that are extracted from the tissue or cell of interest. To inhibit proteolysis, the GenLysates™ are prepared in a lysis buffer containing a complete protease inhibitor cocktail. Each lysate lot is tested by electrophoresis and Western blot analysis.

Supplier: PROVITRO

Isolated T cells, including CD3⁺ pan-T cells, CD4⁺ and CD8⁺ subgroups, have been obtained through immunomagnetic cell separation techniques employing positive or negative selection from mononuclear cells.

Supplier: Charles River Laboratories Cell Solutions

CD56⁺ NK cells are isolated from mononuclear cells either by positive selection using an antibody that targets the CD56 antigen, or by negative selection using immunomagnetic cell separation procedures.

Supplier: ProSci Inc.

Skin tissue lysate (0 Days Old) was prepared by homogenisation in modified RIPA buffer (150 mM sodium chloride, 50 mM Tris-HCl, pH 7,4, 1 mM ethylenediaminetetraacetic acid, 1 mM phenylmethylsulfonyl fluoride, 1% Triton X-100, 1% sodium deoxycholic acid, 0,1% sodium dodecylsulfate, 5 μg/ml of aprotinin, 5 μg/ml of leupeptin.

Supplier: PROVITRO

Isolated T cells, including CD3⁺ pan-T cells, CD4⁺ and CD8⁺ subgroups, have been obtained through immunomagnetic cell separation techniques employing positive or negative selection from mononuclear cells. These differentiated procedures ensure the precise isolation of the intended T-cell groups. CD4⁺ T cells play a role in coordinating immune responses, while CD8⁺ T cells exert a cytotoxic function.

Supplier: Charles River Laboratories Cell Solutions

CD3⁺ T cells are isolated from mononuclear cells by positive selection using immunomagnetic cell separation procedures.

Supplier: ProSci Inc.

Eye tissue lysate (14 Days Old) was prepared by homogenisation in modified RIPA buffer (150 mM sodium chloride, 50 mM Tris-HCl, pH 7,4, 1 mM ethylenediaminetetraacetic acid, 1 mM phenylmethylsulfonyl fluoride, 1% Triton X-100, 1% sodium deoxycholic acid, 0,1% sodium dodecylsulfate, 5 μg/ml of aprotinin, 5 μg/ml of leupeptin.