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773 results for Nucleic Acid Reagents

 

Nucleic Acid Reagents

Nucleic acid reagents form the foundation of genetic analysis and molecular biology, playing a pivotal role from cloning to gene expression studies. Delve into our collection featuring high-fidelity enzymes for end-point PCR, advanced reagents for isothermal amplification, and comprehensive kits for nucleic acid purification. For precision in quantitative assays, explore our selection of qPCR and RT-qPCR kits, primed for unparalleled accuracy. Our portfolio also includes next-generation sequencing reagents, offering cutting-edge solutions for genomic sequencing and personalized medicine advancements.

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Nucleic acid isolation kits, SeraSil-Mag™ beads

Nucleic acid isolation kits, SeraSil-Mag™ beads

Supplier: Cytiva

SeraSil-Mag™ silica coated superparamagnetic beads for nucleic acid isolation deliver high purity DNA extraction in NGS applications. These beads provide an optimal surface for nucleic acid binding with high performance and low background.

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illustra™ MicroSpin™ S-400 HR columns

illustra™ MicroSpin™ S-400 HR columns

Supplier: Cytiva

illustra™ MicroSpin™ S-400 HR columns designed for rapid purification of PCR products (>200 bp) from unincorporated primers (<32-mers) and nucleotides using spin-column chromatography.

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AmMag™ Quatro System Controllers

Supplier: Genscript

The AmMag Quatro is an automated solution for purification of large-scale, high quality, transfection-grade plasmids from Escherichia coli cultures.

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SPINeasy® DNA Pro Kit for Feces, MP Biomedicals

SPINeasy® DNA Pro Kit for Feces, MP Biomedicals

Supplier: MP Biomedicals

The SPINeasy® DNA Pro Kit for Feces is able to handle a wide range of fecal samples.

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SPINeasy RNA kit for tissue, 50 preparations, MP Biomedicals

Supplier: MP Biomedicals

Quick and convenient purification of total RNA from animal tissues, plant tissues and tissue cultures using spin column technology.

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SPINeasy® DNA Pro Kit for Soil, MP Biomedicals

SPINeasy® DNA Pro Kit for Soil, MP Biomedicals

Supplier: MP Biomedicals

Soil samples are complex environments characterised by the presence of inhibitory compounds, such as humic acid, heavy metals, and other aromatic components which may prove to be challenging for downstream analyses.

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SPINeasy® DNA Extraction Kit for Plant, MP Biomedicals

SPINeasy® DNA Extraction Kit for Plant, MP Biomedicals

Supplier: MP Biomedicals

SPINeasy DNA Kit for plant is a silica-membrane spin-column kit that enables quick, convenient and cost-effective isolation of genomic DNA (gDNA) from various plant tissues, such as leaf, stem and seed.

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Magbeads FastRNA kit for FFPE, 192 preparations, MP Biomedicals

Magbeads FastRNA kit for FFPE, 192 preparations, MP Biomedicals

Supplier: MP Biomedicals

MagBeads FastRNA Kit for FFPE is intended for rapid extraction of RNA from tissue, cells, blood, and other clinical samples. RNA can be used directly for RT-PCR, quantitative RT-PCR and so on.

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GeneClean® PCR clean-up, turbo for PCR kit, MP Biomedicals

GeneClean® PCR clean-up, turbo for PCR kit, MP Biomedicals

Supplier: MP Biomedicals

The GeneClean® Turbo for PCR Kit removes primers, nucleotides, enzymes, mineral oil, salts, and other impurities from DNA samples. DNA fragments purified with this kit are ready for direct use in all applications, including sequencing, microarray analysis, ligation and transformation, restriction digestion and labeling.

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illustra™ GFX™ PCR DNA and gel band purification kit

illustra™ GFX™ PCR DNA and gel band purification kit

Supplier: Cytiva

The illustra GFX PCR DNA and Gel Band Purification kits are for the isolation and concentration of DNA fragments from PCR mixtures, DNA-containing agarose gel bands, enzyme-based DNA modifications, and restriction enzyme digests.

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Easy DNA/RNA extraction kit, genesig®

Supplier: PRIMERDESIGN

The genesig easy DNA/RNA extraction protocol begins with a simple lysis step where cells and tissue are lysed to release their nucleic acid. Then minute magnetic particles are added to bind to DNA/RNA. When placed on to the genesig magnetic separator the particles are pulled to the side of the tube making it easy to remove the unwanted supernatant with a pipette. Then a series of simple wash steps are performed before the DNA/RNA is washed off the beads back in to solution, ready for analysis by real-time PCR.

It is fast, and incredibly easy to perform.

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KOD Hot start master mix

Supplier: Merck Millipore (Novagen)

Premixed 2X KOD Hot Start PCR components for convenience and reproducibility.

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ROX Dye qPCR Calibration Solution *10000X*

ROX Dye qPCR Calibration Solution *10000X*

Supplier: AAT BIOQUEST

ROX dye qPCR Calibration Solution supplied as a 10000X concentrate is compatible with a wide variety of qPCR instruments, including the ABI7500 Fast, QuantStudio™, and ViiA™ 7 systems.

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PCR beads

Supplier: EDVOTEK

Each PCR Bead contains Taq DNA polymerase, Taq DNA polymerase buffer, dNTP mixture and MgCl₂. Pack contains sufficient for 25 reactions.

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Q4ever™ Green

Q4ever™ Green

Supplier: AAT BIOQUEST

A real-time polymerase chain reaction (real-time PCR), also known as quantitative polymerase chain reaction (qPCR), is a popular laboratory technique of molecular biology based on the polymerase chain reaction (PCR).

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DNA polymerases, Phusion® hot start II high-fidelity PCR master mix

DNA polymerases, Phusion® hot start II high-fidelity PCR master mix

Supplier: FINNZYMES REAGENTS

Phusion® high-fidelity DNA polymerases set a gold standard for high performance PCR.

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Master mixes, multiplex, Phusion® U

Master mixes, multiplex, Phusion® U

Supplier: FINNZYMES REAGENTS

Phusion® U multiplex PCR master mix is a ready-to-use, 2X end-point PCR master mix designed for simultaneous amplification of multiple targets in a single tube.

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Phusion® High-Fidelity PCR Master Mix with GC Buffer

Phusion® High-Fidelity PCR Master Mix with GC Buffer

Supplier: FINNZYMES REAGENTS

Phusion® High-Fidelity PCR Master Mix with GC Buffer is a convenient 2X mix containing Phusion® DNA polymerase, nucleotides and optimised GC buffer including MgCl₂. Only template and primers need to be added by the user.

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TAQuest™ FAST qPCR Master Mix for TaqMan Probes *Low ROX*

Supplier: AAT BIOQUEST

TAQuest™ FAST qPCR Master mix for TaqMan probes is a ready-to-use 2X solution optimised for qPCR and two-step RT-qPCR well suited to use for TaqMan gene expression assays.

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PCR master mix (2X)

Supplier: Thermo Fisher Scientific

PCR Master Mix is a 2X concentrated solution of Taq DNA polymerase, dNTPs and all other components required for PCR, except DNA template and primers. This pre-mixed formulation saves time and reduces contamination due to a reduced number of pipetting steps required for PCR set up. The mix is optimised for efficient and reproducible PCR.

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Luminaris color Probe qPCR master mixes

Luminaris color Probe qPCR master mixes

Supplier: Thermo Fisher Scientific

Luminaris Color Probe qPCR Master Mixes are universal ready-to-use solutions optimised for qPCR and two-step RT-qPCR. The master mixes include hot start Taq DNA polymerase, uracil-DNA glycosylase (UDG) and dNTPs in an optimised PCR buffer. Only the template, primers and probe need to be added.

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Tissue direct PCR master mix, Phire™

Tissue direct PCR master mix, Phire™

Supplier: FINNZYMES REAGENTS

Phire™ Tissue Direct PCR Master Mix has been developed for amplification of DNA directly from a wide variety of tissues obtained from mice, human, fish, birds and insects. The master mix containing Phire™ Hot Start II DNA polymerase is specially formulated to perform PCR in the presence of different animal tissue-derived inhibitors, such as collagen, melanin and eumelanin (hair, skin) or myoglobin (muscle).

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Taq DNA polymerase (recombinant)

Taq DNA polymerase (recombinant)

Supplier: Thermo Fisher Scientific

Taq DNA Polymerase is a highly thermostable DNA polymerase from the thermophilic bacterium Thermus aquaticus. The enzyme catalyses 5'→3' synthesis of DNA, has no detectable 3'→5' exonuclease (proofreading) activity and possesses low 5'→3' exonuclease activity. In addition, Taq DNA polymerase exhibits deoxynucleotidyl transferase activity, which frequently results in the addition of extra adenines at the 3'-end of PCR products. Recombinant Taq DNA Polymerase is ideal for standard PCR of templates 5 kb or shorter.

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Direct PCR kits

Direct PCR kits

Supplier: FINNZYMES REAGENTS

Direct PCR Kits save time and cost by allowing amplification of DNA directly from the source material. Direct PCR is based on Thermo Scientific Phire® Hot Start II DNA Polymerase and Phusion® Hot Start II High-Fidelity DNA Polymerase. These fusion enzymes are exceptionally fast, robust and highly tolerant of many PCR inhibitors present in unpurified sample material. This allows reliable amplification in challenging conditions where conventional DNA polymerases are completely inhibited.

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DreamTaq™ PCR master mix (2X)

DreamTaq™ PCR master mix (2X)

Supplier: Thermo Fisher Scientific

DreamTaq™ PCR Master Mix (2X) is a ready to use solution containing DreamTaq™ DNA polymerase, optimised DreamTaq™ buffer, MgCl₂ and dNTPs. This pre-mixed formulation saves time and reduces contamination due to the fewer pipetting steps required for PCR set-up. DreamTaq™ DNA polymerase is an enhanced Taq polymerase optimised for high throughput PCR applications. It ensures robust amplification of PCR products up to 6 kb from genomic DNA and up to 20 kb from viral DNA.

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DreamTaq™ Green PCR Master Mix (2X)

DreamTaq™ Green PCR Master Mix (2X)

Supplier: Thermo Fisher Scientific

DreamTaq Green PCR Master Mix (2X) is a ready to use solution containing DreamTaq™ DNA polymerase, optimised DreamTaq Green buffer, MgCl2 and dNTPs. The master mix is supplemented with two tracking dyes and a density reagent that allows for direct loading of the PCR product on a gel. The dyes in the master mix do not interfere with PCR performance and are compatible with downstream applications, such as DNA sequencing, ligation and restriction digestion. The master mix retains all features of DreamTaq DNA polymerase. It is capable for robust amplification up to 6 kb from genomic DNA and up to 20 kb from viral DNA.

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Maxima™ Probe qPCR Master Mix (2X)

Supplier: Thermo Fisher Scientific

The Maxima™ Probe qPCR Master Mix (2X) is a ready to use solution optimised for quantitative real time PCR and two-step real time RT-PCR on most real time PCR machines. The master mix includes Maxima™ hot start Taq DNA polymerase and dNTPs in an optimised PCR buffer. dUTP is included in the mix for optional carryover contamination control using uracil-DNA glycosylase (UDG). The use of Maxima™ probe qPCR master mix (2X) in real time PCR ensures reproducible, sensitive and specific quantification of genomic, plasmid, viral and cDNA templates.

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DNA polymerases, Phusion® U hot start

DNA polymerases, Phusion® U hot start

Supplier: FINNZYMES REAGENTS

Phusion® U DNA polymerase is a novel engineered high fidelity enzyme developed using fusion technology.

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DreamTaq™ DNA polymerase

DreamTaq™ DNA polymerase

Supplier: Thermo Fisher Scientific

DreamTaq™ DNA Polymerase is an enhanced Taq DNA polymerase optimised for all standard PCR applications. It ensures higher sensitivity, longer PCR products and higher yields compared to conventional Taq DNA polymerase. DreamTaq™ DNA Polymerase uses the same reaction set-up and cycling conditions as conventional Taq DNA polymerase. Extensive optimisation of reaction conditions is not required. The enzyme is inhibited by dUTP but can incorporate modified nucleotides. Applications include routine PCR amplification of DNA fragments up to 6 kb from genomic DNA and up to 20 kb from viral DNA, RT-PCR, and generation of PCR products for TA cloning.

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dNTP set, MP Biomedicals

Supplier: MP Biomedicals

Set consists of 100 mM aqueous solutions of dATP, dCTP, dGTP and dTTP each supplied in a separate vial.

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