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773 results for Nucleic Acid Reagents

 

Nucleic Acid Reagents

Nucleic acid reagents form the foundation of genetic analysis and molecular biology, playing a pivotal role from cloning to gene expression studies. Delve into our collection featuring high-fidelity enzymes for end-point PCR, advanced reagents for isothermal amplification, and comprehensive kits for nucleic acid purification. For precision in quantitative assays, explore our selection of qPCR and RT-qPCR kits, primed for unparalleled accuracy. Our portfolio also includes next-generation sequencing reagents, offering cutting-edge solutions for genomic sequencing and personalized medicine advancements.

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Brilliant III Probe MM, Agilent Technologies

Brilliant III Probe MM, Agilent Technologies

Supplier: AGILENT

Brilliant III QPCR and QRT-PCR master mixes increase overall sensitivity and speed through the novel, quick-activating hot start and the engineered Taq-mutant.

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qScript™ XLT One-Step RT-qPCR ToughMix®

qScript™ XLT One-Step RT-qPCR ToughMix®

Supplier: Quantabio

qScript™ XLT One-Step RT-qPCR ToughMix® is a ready to use, highly sensitive master mix for reverse transcription quantitative PCR (RT-qPCR) of RNA templates.

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Brilliant probe multiplex MM

Supplier: AGILENT

The brilliant multiplex QPCR master mix allows you to amplify up to four targets in a single real-time PCR Reaction.

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Perfect Match PCR enhancers

Supplier: AGILENT

Increases specificity and yield of primary PCR amplification products.

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AccuStart™ II PCR Genotyping Kit

AccuStart™ II PCR Genotyping Kit

Supplier: Quantabio

The AccuStart™ II Mouse Genotyping kit is designed for fast and easy preparation of PCR-ready DNA extracts and endpoint PCR analysis from tissues such as tail snips and ear punches commonly used for genotyping knockout and transgenic animals. The kit combines Extracta™ DNA Prep for PCR - Tissue with AccuStart™ II GelTrack™ PCR SuperMix in a single kit.

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MycoSensor qPCR assay kits

Supplier: AGILENT

The MycoSensor PCR and qPCR assay kits detect cultured Mycoplasma infection by real-time qPCR or PCR.

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PerfeCTa® QPCR mixes, SYBR® green super mixes and fast mixes

PerfeCTa® QPCR mixes, SYBR® green super mixes and fast mixes

Supplier: Quantabio

PerfeCTa® SYBR® Green SuperMixes and FastMixes™ enable efficient, sensitive and precise quantitative PCR with proprietary buffers and SYBR® Green stabilisers that maximise fluorescent signal, PCR efficiency, and reduce primer dimers. These SuperMixes and FastMixes™ have been optimised for all Real-Time PCR instrument platforms, including those requiring normalisation with ROX reference dye or fluorescein.

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Qscript lyo 1-step

Qscript lyo 1-step

Supplier: Quantabio

Qscript lyo 1-step is a lyophilised single-reaction reagent optimised for highly sensitive and reproducible one-step RT-qPCR using hydrolysis probes.

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PerfeCTa® qPCR FastMix® II

Supplier: Quantabio

PerfeCTa® qPCR FastMix® II is an advanced qPCR reagent system for both fast and conventional PCR cycling protocols or instruments. It is a versatile and robust solution that provides the ultimate sensitivity and high PCR efficiency using a variety of fluorogenic probe chemistries, including TaqMan® hydrolysis probes. PerfeCTa® qPCR FastMix® II is provided as a 2X concentrated ready to use reaction cocktail that contains all required reaction components, except primers, probe(s), and DNA template. The light blue colour of the AccuVue™ tracer dye simplifies reaction assembly in white, or clear, plates and helps to minimise pipetting or mixing errors. It does not interfere with qPCR performance or affect the stability of the product.

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Fusion DNA polymerases, Herculase II

Supplier: AGILENT

Herculase II Fusion DNA Polymerases produce superior yields with extension times as short as 15 s/kb, even with genomic DNA targets up to 23 kb.

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Virus detection kit, qScript™ 1-Step Virus ToughMix®

Virus detection kit, qScript™ 1-Step Virus ToughMix®

Supplier: Quantabio

Superior sensitivity for viral RNA detection.

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Brilliant II probe MM with ROX

Supplier: AGILENT

Brilliant II QPCR and QRT-PCR reagents were developed to maintain the high quality of original Brilliant product line, but with a significant improvement in sensitivity of detection, ensuring reproducible quantification even at low target concentrations.

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AccuStart™ II PCR ToughMix®

AccuStart™ II PCR ToughMix®

Supplier: Quantabio

AccuStart™ II PCR ToughMix® is a 2X concentrated ready to use reaction cocktail for PCR amplification of DNA templates that overcomes many known inhibitors of PCR often present in crude samples extracted from environmental specimens, plant tissues, or animal tissues. It contains all components, except primers and template.

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PerfeCTa® MultiPlex qPCR SuperMix, low ROX

Supplier: Quantabio

PerfeCTa® Multiplex qPCR SuperMix, low ROX is a 2X concentrated, ready to use reaction cocktail that contains all components, except primers, probe(s), and template for real time qPCR on Applied Biosystems 7500 or Stratagene MX series of real time PCR systems.

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VWR® Probe qPCR Master Mix

VWR® Probe qPCR Master Mix

Supplier: VWR Chemicals

VWR® Probe qPCR Master Mix features a distinctive universal passive reference dye that is compatible with ROX-dependent and ROX-independent qPCR instruments.

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AccuStart™ Genotyping ToughMix®

AccuStart™ Genotyping ToughMix®

Supplier: Quantabio

AccuStart™ Genotyping ToughMix® is a 2X concentrated ready to use reaction cocktail for PCR amplification of DNA templates that overcomes inhibitors often present in crude samples extracted from environmental specimens, plant tissues, or animal tissues. It is a versatile and robust PCR reagent that provides highly specific and efficient amplification for genotyping sequence variants including allelic discrimination of single nucleotide polymorphisms (SNPs) using a variety of fluorogenic probe chemistries, including TaqMan® hydrolysis probes.

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qPCR SuperMix, PerfeCTa® MultiPlex

Supplier: Quantabio

This SuperMix is a 2X concentrated, ready to use reaction cocktail for Real-Time qPCR that contains all components, except primers, probes, and templates. The system transcends multiplex limitations of conventional PCR master mixes, enabling unbiased amplification of up to five target sequences in a single tube. Suppression of low copy amplicons by high copy reference targets in the amplification is a common problem in multiplex PCR. This can skew, or mask the apparent representation and quantification of low copy target sequences. PerfeCTa® MultiPlex qPCR SuperMix delivers dynamic range and sensitivity to multiplexed qPCR that is comparable to that for singleplex qPCR probe assays without the need for limiting or variable primer concentrations.

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PerfeCTa® qPCR ToughMix®

Supplier: Quantabio

PerfeCTa® qPCR ToughMix® is a 2X concentrated ready to use reaction cocktail for PCR amplification of DNA templates that overcomes many known inhibitors of PCR often present in crude samples extracted from environmental specimens, plant tissues or animal tissues. It is a versatile and robust Real-Time qPCR reagent that provides maximum sensitivity and PCR efficiency with a variety of fluorogenic probe chemistries, including TaqMan® hydrolysis probes. PerfeCTa® qPCR ToughMix® contains all required reaction components, except primers, probe(s) and DNA template. The light blue colour of the AccuVue™ tracer dye simplifies reaction assembly in white, or clear plates and helps to minimise pipetting or mixing errors. It does not interfere with qPCR performance or affect the stability of the product.

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Pfu DNA polymerase

Supplier: AGILENT

High-quality polymerases for difficult to amplify DNA targets.

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VWR®, Real-time PCR kit for Listeria Monocytogenes

VWR®, Real-time PCR kit for Listeria Monocytogenes

Supplier: VWR Chemicals

This kit is designed to detect the pathogen microorganism Listeria monocytogenes in food samples, after enrichment steps, according to standard microbiology procedures. It can also be used to confirm suspect colonies isolated in selective culture media.

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eQo One-Step ToughMix®

eQo One-Step ToughMix®

Supplier: Quantabio

No compromises. Unparalleled performance meets sustainability.

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DNA polymerase, hotstart, PfuUltra II Fusion

Supplier: AGILENT

This DNA polymerase is ideal for a variety of techniques requiring high-fidelity DNA synthesis by the polymerase chain reaction (PCR).

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AccuStart™ II GelTrack PCR SuperMix

Supplier: Quantabio

AccuStart™ II GelTrack PCR SuperMix is a 2X concentrated, ready-to-use reaction cocktail for routine PCR amplification of DNA fragments up to 4 kb followed by analysis on agarose gels. It contains all components, except primers and template. AccuStart™ II GelTrack PCR SuperMix simplifies reaction assembly, improves assay reproducibility, and reduces the risk of contamination. The supermix includes electrophoresis tracking dyes that migrate at approximately 4 kb and 50 bp to allow direct loading of PCR product on agarose gels following amplification. AccuStart™ II Taq DNA polymerase in the master mix is inactivated with monoclonal antibodies that bind the polymerase and keep it inactive prior to the initial PCR denaturation step. Upon heat activation (1 minute at 94 °C), the antibodies denature irreversibly, releasing fully active, unmodified Taq DNA polymerase. This enables specific and efficient primer extension with the convenience of room temperature reaction assembly.

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repliQa HiFi ToughMix

repliQa HiFi ToughMix

Supplier: Quantabio

Superior speed and inhibitor tolerance.

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Brilliant II SYBR® master mix with ROX

Supplier: AGILENT

Brilliant II SYBR® QPCR and QRT-PCR master mix kits with high/low Rox offer superior sensitivity for improved quantification and reproducibility.

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VWR®, Taq DNA polymerase, 500 U

VWR®, Taq DNA polymerase, 500 U

Supplier: VWR Chemicals

Taq DNA Polymerase is ideal for general PCR and primer extension reactions to amplify DNA templates with high sensitivity. PCR can be initiated from as few as 6 primed templates. Simply add 0,5 µl of Taq DNA Polymerase to a 25 µl PCR reaction.

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Brilliant III SYBR® MM, Agilent Technologies

Brilliant III SYBR® MM, Agilent Technologies

Supplier: AGILENT

Brilliant III Ultra-Fast SYBR® QPCR and QRT-PCR Master Mixes deliver superior specificity by utilizing a novel faster-activating hot start method to minimize the formation of primer-dimers and other off-target reactions.

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Yeast transformation kit, Fast Yeast Transformation™

Yeast transformation kit, Fast Yeast Transformation™

Supplier: G-Biosciences

Fast Yeast Transformation™ is a rapid single step yeast transformation kit that takes less than 10 minutes to prepare competent yeast cells. The competent yeast cells can be used immediately or frozen for later use. This method is suitable for both circular and linear plasmid transformations.

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Electroporation solution kits, BTXpress™

Electroporation solution kits, BTXpress™

Supplier: BTX

The BTXpress™ is a single buffer solution, developed to quickly and efficiently deliver genes into mammalian cells that were previously considered hard to transfect by chemical and other non viral methods. Transfection using this high performance electroporation solution is equally effective in delivering DNA as well as siRNA into mammalian cells.

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Flatpack chambers

Flatpack chambers

Supplier: BTX

Flatpack chambers are primarily used for prokaryotic applications (bacterial or yeast transformations), however they are also often used for high efficiency stem cell transfection.

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