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Nucleic Acid Reagents

Nucleic acid reagents form the foundation of genetic analysis and molecular biology, playing a pivotal role from cloning to gene expression studies. Delve into our collection featuring high-fidelity enzymes for end-point PCR, advanced reagents for isothermal amplification, and comprehensive kits for nucleic acid purification. For precision in quantitative assays, explore our selection of qPCR and RT-qPCR kits, primed for unparalleled accuracy. Our portfolio also includes next-generation sequencing reagents, offering cutting-edge solutions for genomic sequencing and personalized medicine advancements.

Restriction enzymes, NcoI, FastDigest™, fermentas

Restriction enzymes, NcoI, FastDigest™, fermentas

Supplier: Thermo Fisher Scientific

Thermo Scientific™ FastDigest™ enzymes are an advanced line of restriction enzymes for rapid DNA digestion.

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Virus detection kit, qScript™ 1-Step Virus ToughMix®

Virus detection kit, qScript™ 1-Step Virus ToughMix®

Supplier: Quantabio

Superior sensitivity for viral RNA detection.

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TriLink® Sulf Cyanine5 Aminoallyluridine-5'-Triphosphate

TriLink® Sulf Cyanine5 Aminoallyluridine-5'-Triphosphate

Supplier: TriLink BioTechnologies

Cyanine 5-Aminoallyluridine-5'-Triphosphate is a substrate for T7 RNA polymerase, allowing the incorporation of cyanine 5 into RNA and mRNA during in vitro transcription. Cyanine 5 incorporation allows for fluorescent detection of RNA and mRNA independent of protein translation.

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TriLink® N1-Methyl-Pseudouridine-5'-Triphosphate

TriLink® N1-Methyl-Pseudouridine-5'-Triphosphate

Supplier: TriLink BioTechnologies

N1-Methyl-Pseudouridine-5'-Triphosphate is widely used in mRNA synthesis for therapeutic and vaccine development to improve mRNA functionality. This nucleotide consists of a modified base, a ribose, and a 5′ triphosphate group.

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TriLink® CleanScribe™ RNA Polymerase

TriLink® CleanScribe™ RNA Polymerase

Supplier: TriLink BioTechnologies

CleanScribe™ RNA Polymerase is a novel enzyme that reduces double-stranded RNA (dsRNA) formation in IVT RNA synthesis.

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Restriction enzymes, BamHI, HC, Fermentas

Restriction enzymes, BamHI, HC, Fermentas

Supplier: Thermo Fisher Scientific

GATCC sites and cuts best at 37 °C in its own unique buffer.

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AMPIGENE® qPCR 1-Step Green Kit Hi-ROX

Supplier: ENZO LIFE SCIENCES

Optimised and efficient cDNA synthesis and probe-based qPCR in a single tube.

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SAVIEW® PLUS HRP Reagent

Supplier: ENZO LIFE SCIENCES

High sensitivity, low background streptavidin-based nanopolymer detection reagent for use with HIGHDEF® chromogens in ISH and IHC applications.

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Porcine detection kit

Porcine detection kit

Supplier: AGILENT

Detect as low as 300 fg of Porcine DNA in food and pharmaceutical samples, including gel caps.

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Bio-11-CTP

Bio-11-CTP

Supplier: ENZO LIFE SCIENCES

Bio-11-CTP (Biotin-11-cytidine-5’-triphosphate) can replace CTP for in vitro transcription reaction catalysed by T3, T7 or SP6 RNA polymerases.

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Bio-16-UTP

Bio-16-UTP

Supplier: ENZO LIFE SCIENCES

Bio-16-UTP (Biotin-16-uridine-5’-triphosphate) can replace UTP for in vitro transcription reaction catalysed by T3, T7 or SP6 RNA polymerases.

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AMPIGENE® HS Taq Mix

Supplier: ENZO LIFE SCIENCES

Increased sensitivity standard PCR mix for a broader range of samples, with enhanced speed, yield, and specificity.

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AMPIGENE® qPCR 1-Step Probe Kit Hi-ROX

Supplier: ENZO LIFE SCIENCES

Optimised and efficient cDNA synthesis and probe-based qPCR in a single tube.

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Taq extender PCR additive

Supplier: AGILENT

Improves reliability and yield of conventional Taq-based PCR amplifications.

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TriLink® CleanCap® Reagent AG (3'OMe)

TriLink® CleanCap® Reagent AG (3'OMe)

Supplier: TriLink BioTechnologies

TriLink's patented CleanCap® Reagent AG (3' OMe) is a co-transcriptional capping reagent for in vitro transcription of 5' capped mRNA resulting in a Cap 1 structure with >95% capping efficiency.

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TriLink® CleanCap® Reagent  AG

TriLink® CleanCap® Reagent AG

Supplier: TriLink BioTechnologies

CleanCap® Reagent AG is TriLink’s patented co-transcriptional capping reagent for in vitro transcription of 5’ capped mRNA resulting in a Cap 1 structure. CleanCap has shown to provide up to 98% capping efficiency.

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Blur 8 BioProbe®

Supplier: ENZO LIFE SCIENCES

The Blur 8 BioProbe® labeled probe is prepared by nick translation of a clone of the human alu repeat sequence. Fragment size range: 100-1000 base pairs (as estimated by agarose gel electrophoresis).

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Cloned Pfu DNA polymerase alternative detergents

Supplier: AGILENT

Cloned Pfu DNA Polymerase for high fidelity and difficult, GC-Rich PCR.

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TriLink® Uridine-5'-Triphosphate

TriLink® Uridine-5'-Triphosphate

Supplier: TriLink BioTechnologies

This nucleotide consists of an unmodified base, a ribose, and a 5′ triphosphate group for synthesis of RNA molecules.

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AMPIGENE® cDNA synthesis kit

AMPIGENE® cDNA synthesis kit

Supplier: ENZO LIFE SCIENCES

AMPIGENE® cDNA Synthesis Kit for efficient and sensitive cDNA synthesis, for downstream qPCR.

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Restriction enzymes, NotI, 10 U/µl, Fermentas

Restriction enzymes, NotI, 10 U/µl, Fermentas

Supplier: Thermo Fisher Scientific

GGCCGC sites and cuts best at 37 °C in O buffer.

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VWR®, TEMPase Hot Start 2× master mix

VWR®, TEMPase Hot Start 2× master mix

Supplier: VWR Chemicals

TEMPase Hot Start DNA Polymerase Master Mix and Blue TEMPase Master Mix are good alternatives to TEMPase Hot Start DNA Polymerase. These master mixes offer easy reaction assembly at room temperature, reduced set-up time and fewer handling steps, which lead to increased reproducibility. As a consequence TEMPase Hot Start DNA Polymerase Master Mix is highly suited to standard tests.

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AccuStart™ master mixes, long range SuperMix

AccuStart™ master mixes, long range SuperMix

Supplier: Quantabio

Superior sensitivity and multiplexing for DNA amplification of long targets.

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T4 DNA polymerase

T4 DNA polymerase

Supplier: Thermo Fisher Scientific

T4 DNA Polymerase catalyses the 5'→3' synthesis of DNA from a primed single-stranded DNA template.

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VWR®, GC Rich TEMPase Hot start DNA polymerase and MasterMix

VWR®, GC Rich TEMPase Hot start DNA polymerase and MasterMix

Supplier: VWR Chemicals

VWR® TEMPase Hot Start DNA Polymerases are highly stable polymerases, featuring higher specificity, superior sensitivity and greater yields compared to standard DNA polymerases. These features make them well suited for the detection of low abundance targets. Other uses include screening, amplification of GC-rich sequences, multiplex PCR, direct PCR and qPCR.

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Restriction enzymes, PfoI, Fermentas

Restriction enzymes, PfoI, Fermentas

Supplier: Thermo Fisher Scientific

CCNGGA sites and cuts best at 37 °C in Tango buffer.

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PfuTurbo DNA polymerase

Supplier: AGILENT

This DNA polymerase is ideal for a variety of techniques requiring high-fidelity DNA synthesis by the polymerase chain reaction (PCR).

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VWR®, PCR buffers

VWR®, PCR buffers

Supplier: VWR Chemicals

An optimal buffer system is critical for the performance of successful PCR. VWR® Taq DNA Polymerase kits generally include two different buffers, Key Buffer and Extra Buffer, which are suited for different PCR needs. All buffers contain Tris and 15 mM MgCl₂ (1,5 mM MgCl₂ final concentration). Additional MgCl₂ for easy optimisation is included in a separate vial.

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Restriction enzymes, BpiI, FastDigest™, fermentas

Restriction enzymes, BpiI, FastDigest™, fermentas

Supplier: Thermo Fisher Scientific

Thermo Scientific™ FastDigest™ enzymes are an advanced line of restriction enzymes for rapid DNA digestion.

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Restriction enzymes, SalI, FastDigest™, Fermentas

Restriction enzymes, SalI, FastDigest™, Fermentas

Supplier: Thermo Fisher Scientific

Thermo Scientific™ FastDigest™ enzymes are an advanced line of restriction enzymes for rapid DNA digestion.

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