You searched for: Protein Purification
Protein purification systems are intended to isolate one or multiple proteins from a complex mixture. Working with cells, tissues, or whole organisms, the machines purify with predefined start points and editable method templates. With a single click, the compact designed equipment offers real-time run control and visualization. Suitable for even cold-room operations, the products enable you to perform purification of affinity-tagged proteins, antibodies, untagged or native proteins, as well as sample clean-up.
Affinity chromatography media, HiTrap™ Capto™ Lentil Lectin
Supplier: Cytiva
Capto™ Lentil Lectin is an affinity chromatography resin for purification of glycoproteins and other molecules containing carbohydrates such as α-D-mannose and α-D-glucose or sterically related residues.
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Protease affinity chromatography media, PreScission™, Cytiva
Supplier: Cytiva
Enables the low-temperature cleavage of fusion proteins containing the PreScission Protease recognition sequence.
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Affinity coupling media, CNBr-Activated Sepharose™ 4B
Supplier: Cytiva
CNBr-activated Sepharose™ 4B is pre-activated media used for coupling antibodies or other large proteins containing -NH₂ groups to the Sepharose™ media, by the cyanogen bromide method, without an intermediate spacer arm.
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Multimodal chromatography media, Capto™ MMC
Supplier: Cytiva
Capto™ MMC is a multimodal cation exchanger with a different selectivity, compared with traditional ion exchangers.
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Hydrophobic interaction chromatography media, Capto™ Butyl
Supplier: Cytiva
Capto™ Butyl is used in the capture and intermediate stages of protein purification. The combination of high capacity, high flow rate and low back pressure reduces process cycle times.
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Ion exchange chromatography media, Capto™ S
Supplier: Cytiva
Capto™ S is a strong cation exchange bioprocessing medium, designed to meet the demands of modern large-scale manufacturers for fast, efficient, and cost-effective capture and intermediate protein purification when high volume throughput is essential.
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Ion exchange chromatography media, Capto™ Q
Supplier: Cytiva
Capto™ Q is a strong anion exchange bioprocess medium, designed to meet the demands of modern large-scale manufacturers for fast, efficient, and cost-effective capture and intermediate protein purification when high volume throughput is essential.
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Gelatin-Sepharose® 4B
Supplier: Cytiva
Gelatin Sepharose® 4B affinity media with immobilised gelatin is designed for the purification or depletion of fibronectin as gelatin binds to fibronectin with high specificity. Gelatin Sepharose® 4B is bovine gelatin coupled to Sepharose® 4B by the cyanogen bromide method.
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Affinity chromatography media, Streptavidin Sepharose™ High Performance
Supplier: Cytiva
Streptavidin Sepharose™ High Performance is an affinity chromatography medium designed for fast and reliable, high resolution purification of biotinylated biomolecules. Purified Streptavidin isolated from Streptomyces avidinii is immobilised on Sepharose™ High Performance.
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Protein G Sepharose™ 4 Fast Flow Affinity Chromatography Media
Supplier: Cytiva
Protein G Sepharose 4 Fast Flow has recombinant protein G immobilized by the cyanogen bromide (CNBr) method to Sepharose 4 Fast Flow.
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Affinity chromatography media, Lentil Lectin Sepharose™ 4B
Supplier: Cytiva
Lentil lectin is a metalloprotein, containing Ca₂₊ and Mn₂₊, which binds reversibly to polysaccharides and glycoconjugates containing glucose or mannose type sugars.
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Affinity purification media, GammaBind™ Plus Sepharose™
Supplier: Cytiva
GammaBind™ Plus Sepharose™ is GammaBind G, type 3, covalently immobilised to Sepharose™ CL-6B by malimide linkage.
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DEAE Sephadex™ A-50 Ion Exchange Chromatography Media, Cytiva
Supplier: Cytiva
DEAE Sephadex™ A-25 and DEAE Sephadex™ A-50 are weak anion exchangers. Sephadex™ ion exchangers are produced by introducing functional groups onto the cross-linked dextran matrix. These groups are attached to glucose units in the matrix by stable ether linkages.
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Gel filtration media, Sephacryl™ High Resolution
Supplier: Cytiva
Sephacryl™ High Resolution (HR) gel filtration media allow fast and reproducible purification of proteins, polysaccharides, and other macromolecules by gel filtration at laboratory and industrial scale.
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Butyl-S Sepharose™ 6 Fast Flow Hydrophobic interaction chromatography media
Supplier: Cytiva
Butyl-S Sepharose™ 6 Fast Flow is composed of cross-linked 6% agarose beads modified with butyl groups via a thio-ether linkage. This resin is designed for the binding and elution of relatively strong hydrophobic molecules at comparatively low salt concentrations.
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Hydrophobic interaction chromatography media, Phenyl Sepharose™ High Performance
Supplier: Cytiva
Phenyl Sepharose™ High Performance is an aromatic hydrophobic interaction chromatography (HIC) medium, designed for intermediate and polishing step purification steps when high resolution has priority.
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Ion exchange chromatography media, ANX Sepharose™ 4 Fast Flow (High Sub)
Supplier: Cytiva
ANX Sepharose™ 4 Fast Flow (high sub) is a weak anion exchanger, with a different selectivity compared with traditional ion exchangers.
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Ion exchange chromatography media, Q Sepharose™ High Performance
Supplier: Cytiva
Q Sepharose™ High Performance is composed of crosslinked agarose beads with a mean diameter of 34 µm, modified with quaternary (Q) strong anion exchange groups.
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Ion exchange chromatography media, DEAE Sepharose™ CL-6B
Supplier: Cytiva
DEAE Sepharose™ CL-6B is a weak anion exchanger.
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Sepharose™ CL-2B Gel Filtration Base Matrix
Supplier: Cytiva
Sepharose CL-2B is a well-proven cross-linked agarose gel filtration base matrix and is frequently used for coupling affinity ligands to the matrix. The matrix is not pre-activated and the user performs all steps in coupling.
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Affinity chromatography media, Streptavidin Sepharose™ High Performance
Supplier: Cytiva
Streptavidin Sepharose™ High Performance is an affinity chromatography resin designed for fast and reliable, high resolution purification of biotinylated biomolecules.
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Accessories and spare parts for ÄKTApilot
Supplier: Cytiva
TC gasket, 25/4
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Gel filtration calibration kits
Supplier: Cytiva
Gel Filtration Calibration Kits are suitable for calibration of high-resolution gel filtration media and columns, such as Superdex, Superose and Sephacryl.
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Affinity chromatography media, IMAC Sepharose™ 6 Fast Flow
Supplier: Cytiva
IMAC Sepharose 6 Fast Flow is composed of cross-linked 6% agarose beads modified with a novel chelating ligand immobilized to the base matrix.
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Affinity chromatography media, MabSelect Xtra™
Supplier: Cytiva
MabSelect Xtra has the same recombinant Protein A ligand as MabSelect but is based on a high-flow agarose base matrix with increased porosity and a slightly decreased particle size compared to MabSelect.
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Phenyl Sepharose™ 6 Fast Flow (Low Sub) Hydrophobic Interaction Chromatography Media
Supplier: Cytiva
Phenyl Sepharose 6 Fast Flow is a well established, standard aromatic hydrophobic interaction chromatography (HIC) resin for capture and intermediate purification requiring low to resin hydrophobicity
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Gel filtration media, Sephadex™ G-10
Supplier: Cytiva
Sephadex™ G-10 is a well established gel filtration medium for desalting and buffer exchange of peptides and small biomolecules >700 molecular weight.
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Capto™ adhere ImpRes multimodal chromatography media, Cytiva
Supplier: Cytiva
Capto™ adhere ImpRes is a strong anion exchange multimodal BioProcess chromatography medium (resin), designed to meet the demands of modern large-scale manufacturers, for high-resolution polishing of monoclonal antibodies (MAbs) and other biomolecules. Capto™ adhere ImpRes is designed to allow effective MAb polishing in the second or third step of a purification scheme downstream of the protein A capture step. Polishing can be performed in either bind and elute (binding) or flowthrough (non binding) modes.
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Affinity purification media, rProtein A Sepharose™ Fast Flow
Supplier: Cytiva
rProtein A Sepharose™ 4 Fast Flow is a well established antibody affinity medium is designed for the purification of monoclonal and polyclonal antibodies based on Sepharose™ Fast Flow platform. The recombinant protein A is produced in E.coli and engineered for an oriented coupling to giving a matrix with enhanced binding capacity. The epoxy-based coupling ensures low ligand leakage. The specificity of the recombinant protein A for the Fc region of IgG is similar to native protein A and provides excellent purification in one step. The high capacity, low ligand leakage and a well established base matrix makes rProtein A Sepharose™ Fast Flow ideal for purification of monoclonal antibodies at both laboratory and process scale.
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Affinity chromatography media, Chelating Sepharose™ Fast Flow
Supplier: Cytiva
Chelating Sepharose Fast Flow is composed of cross-linked 6% agarose beads modified with iminodiacetic immobilized to the base matrix by stable ether linkages and sufficiently long spacer arms.