You searched for: Protein Purification
Protein purification systems are intended to isolate one or multiple proteins from a complex mixture. Working with cells, tissues, or whole organisms, the machines purify with predefined start points and editable method templates. With a single click, the compact designed equipment offers real-time run control and visualization. Suitable for even cold-room operations, the products enable you to perform purification of affinity-tagged proteins, antibodies, untagged or native proteins, as well as sample clean-up.
Direct IP kit, Pierce™
Supplier: Thermo Fisher Scientific
The Pierce™ Direct IP Kit uses an activated resin to covalently immobilise IP antibodies (any species or class) on agarose beads without the aid of Protein A/G, enabling immunoprecipitation without antibody interference.
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Enterokinase cleavage capture kit
Supplier: Merck Millipore (Novagen)
The Enterokinase Cleavage Capture Kit is designed for highly specific cleavage of fusion proteins followed by rapid, affinity-based capture and removal of enterokinase. Following cleavage of the target protein, rEK is removed with >99% efficiency from the reaction by affinity capture on EKapture™ Agarose. Following capture of rEK, the EKapture Agarose is removed by spin filtration, because the same buffer conditions are used for both cleavage and capture no buffer changes are necessary.
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Pegylation reagents, methyl-PEG-amine compounds
Supplier: Thermo Fisher Scientific
The methyl-PEGn-amine (MA[PEG]n) PEGylation reagents are methyl ether-terminated PEG amines that are used for modifying proteins or surfaces such as beads, nanoparticles and self-assembled monolayers. These PEGylation reagents are homogenous compounds of defined molecular weight and spacer length, providing precision in optimising modification applications.
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Protein purification system, ÄKTA™ start
Supplier: Cytiva
ÄKTA start is an easy to use chromatography system that automates manual purification procedures using, for example, HiTrap columns. It offers an entry level chromatography solution for the preparative purification of proteins at lab scale.
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Affinity chromatography media, Dextrin Sepharose™ High Performance
Supplier: Cytiva
Dextrin Sepharose™ High Performance is an affinity media for purification of recombinant proteins tagged with Maltose Binding Protein (MBP).
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Affinity chromatography media, Glutathione Sepharose™ 4B
Supplier: Cytiva
Glutathione Sepharose™ 4B is designed for high capacity single-step purification of Glutathione S-Transferase (GST) tagged fusion proteins.
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Affinity chromatography media, TALON® Superflow™
Supplier: Cytiva
Cobalt-based chromatography medium designed for purification of histidine-tagged recombinant proteins by immobilised metal affinity chromatography (IMAC).
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Affinity chromatography media, Ni Sepharose™ High Performance
Supplier: Cytiva
Ni Sepharose™ High Performance affinity media for high resolution, high capacity purification of histidine-tagged proteins by immobilised metal ion affinity chromatography (IMAC).
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Affinity chromatography media, Capto™ Blue
Supplier: Cytiva
Capto™ Blue is an affinity medium for the capture of albumin, purification of enzymes, and recombinant proteins at laboratory and process scales.
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Multimodal chromatography media, Capto™ Core 700
Supplier: Cytiva
Capto™ Core 700 is designed for intermediate purification and polishing of viruses and other large biomolecules (molecular weight >700000).
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Hydrophobic interaction chromatography media, Capto™ Phenyl (high sub)
Supplier: Cytiva
Capto™ Phenyl (high sub) is used in the capture and intermediate stages of protein purification.
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Accessories for ÄKTA chromatography systems
Supplier: Cytiva
Inlet filter set, PP
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Butyl Sepharose™ High Performance Hydrophobic interaction chromatography media
Supplier: Cytiva
Butyl Sepharose™ HP media is particularly well suited for intermediate purification and polishing steps providing high resolution due to the small particle size.
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Affinity chromatography media, Glutathione Sepharose™ 4 Fast Flow
Supplier: Cytiva
Glutathione Sepharose 4 Fast Flow is used for the purification of GST-tagged proteins from bacterial lysates and other S-tranferases or glutathione-dependent proteins.
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Affinity chromatography media, Blue Sepharose™ High Performance
Supplier: Cytiva
This media is suitable for affinity chromatography.
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Multimodal chromatography media, Capto™ MMC
Supplier: Cytiva
Capto™ MMC is a multimodal cation exchanger with a different selectivity, compared with traditional ion exchangers.
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Affinity chromatography media, Glutathione Sepharose™ High Performance
Supplier: Cytiva
Glutathione Sepharose High Performance is used for the purification of GST-tagged proteins from bacterial lysates and other S-transferases or glutathione-dependent proteins.
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Sepharose Chromatography Resins, Strep-Tactin® XT
Supplier: Cytiva
A chromatography resin for purification of recombinant proteins tagged with Strep-tag II and Twin-Strep-tag. These proteins bind very specifically to the immobilised Strep-Tactin XT ligand giving highly pure target protein.
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Gelatin-Sepharose® 4B
Supplier: Cytiva
Gelatin Sepharose® 4B affinity media with immobilised gelatin is designed for the purification or depletion of fibronectin as gelatin binds to fibronectin with high specificity. Gelatin Sepharose® 4B is bovine gelatin coupled to Sepharose® 4B by the cyanogen bromide method.
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Phenyl Sepharose™ 6 Fast Flow (High Sub) Hydrophobic Interaction Chromatography Media
Supplier: Cytiva
Phenyl Sepharose 6 Fast Flow is a well established, standard aromatic hydrophobic interaction chromatography (HIC) resin for capture and intermediate purification requiring low to resin hydrophobicity
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Affinity chromatography media, Streptavidin Sepharose™ High Performance
Supplier: Cytiva
Streptavidin Sepharose™ High Performance is an affinity chromatography medium designed for fast and reliable, high resolution purification of biotinylated biomolecules. Purified Streptavidin isolated from Streptomyces avidinii is immobilised on Sepharose™ High Performance.
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Hydrophobic interaction chromatography media, Capto™ SP ImpRes
Supplier: Cytiva
Capto™ SP ImpRes is a strong cation exchange bioprocessing medium, designed to meet the demands of modern large-scale manufacturers for fast, efficient, and cost-effective intermediate and polishing protein purification.
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Ion exchange chromatography media, Capto™ Q ImpRes
Supplier: Cytiva
Capto™ Q ImpRes is a strong anion exchange bioprocess medium, designed to meet the demands of modern large-scale manufacturers for fast, efficient, and cost-effective intermediate and polishing protein purification.
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Accessories for chromatography systems
Supplier: Cytiva
UniTag sheet with 108 labels
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Multimodal chromatography media, Capto™ adhere
Supplier: Cytiva
Capto™ adhere is a multimodal anion exchanger for intermediate purification and polishing of monoclonal antibodies after the Protein A capture step, giving the possibility to design a two-step chromatographic downstream process.
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Affinity chromatography media, IMAC Sepharose™ High Performance
Supplier: Cytiva
IMAC Sepharose High Performance media is easily charged by the user with the metalion of choice for Immobilized Metal ion Affinity Chromatography (IMAC) and subsequent purification of polyhistidine tagged proteins.
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Affinity chromatography media, HiTrap™ Capto™ Lentil Lectin
Supplier: Cytiva
Capto™ Lentil Lectin is an affinity chromatography resin for purification of glycoproteins and other molecules containing carbohydrates such as α-D-mannose and α-D-glucose or sterically related residues.
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Protease affinity chromatography media, PreScission™, Cytiva
Supplier: Cytiva
Enables the low-temperature cleavage of fusion proteins containing the PreScission Protease recognition sequence.
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Hydrophobic interaction chromatography media, Capto™ Butyl
Supplier: Cytiva
Capto™ Butyl is used in the capture and intermediate stages of protein purification. The combination of high capacity, high flow rate and low back pressure reduces process cycle times.