You searched for: Protein Purification Resins
Immobilised metal ion affinity chromatography (IMAC) media, Ni Sepharose™ excel
Supplier: Cytiva
Ni Sepharose™ excel is an IMAC medium pre-charged with nickel ions very strongly bound to a chelating ligand. Samples that usually cause stripping of metal ions can, therefore, be loaded to the medium. Ni Sepharose™ excel is designed primarily for capture and purification of histidine-tagged proteins secreted into cell culture supernatants from eukaryotic cells such as insect cells or CHO cells.
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Process media, Fractogel® EMD, Millipore
Supplier: Merck
Fractogel® presents a range of media with 'tentacular' geometries which provide an enhancement of surface topology that give the resins advantageous features for many production processes. The resulting strong binding of biomolecules by a variety of ligands (depending on application and media chosen) and the long lifetime of the material leads to high efficiency and economy of all Fractogel® media. The process media, based on a synthetic methacrylate resin, have high mechanical strength excellent and high chemical stability making the media easy to pack and ‘clean in place'. In addition the base has several advantages over carbohydrate supports as the polymer resin is resistant to microbial degradation and the risk of contamination with endotoxins is greatly reduced.
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Chelex® 100, sodium form
Supplier: Merck
Chelex® 100 Chelating Resin is a weak cation exchange resin that has high selectivity, approximately 5,000:1, for copper, iron, and other heavy metal ions over monovalent cations. Chelex 100 Resin is made of a styrene divinylbenzene copolymer containing paired iminodiacetate ions, which act as chelating groups in binding polyvalent metal ions.
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Gel filtration media, Sephadex® G-50
Supplier: Merck
Sephadex® G-50 is a gel filtration medium used in affinity chromatography, protein chromatrography and gel filtration chromatography.
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Ion exchange chromatography media, SP Sepharose® fast flow
Supplier: Merck
Sepharose Fast Flow is composed of macroporous, beaded, cross-linked agarose and modified with sulphopropyl (SP) strong cation exchange groups which remains charged and maintains consistently high capacity over the entire working range of pH 4 to 13.
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Affinity chromatography media, gelatin-agarose
Supplier: Merck
Gelatin-agarose is an agarose in saline suspension used for affinity chromatography, protein chromatography and specialty resins.