You searched for: Enzyme Assays
Either measuring substrate consumption or overall production, the enzyme assays are used to monitor enzymatic activity. Provided with the required supplies and reagents for any preferred method, enzyme kinetics and inhibition studies can be successfully completed. While continuous assay sampling methods give activity readings throughout development, discontinuous techniques calculate after the reaction has been stopped. The standardized enzyme assays will provide accurate identification and measurement of the protein molecules present in samples.
Amplite® Fluorimetric Total Thiol Quantitation Assay Kit *Green Fluorescence*
Supplier: AAT BIOQUEST
The detection and measurement of free thiol (such as free cysteine, glutathione and cysteine residues in proteins) is one of the essential tasks for investigating biological processes and events in many biological systems.
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Amplite® Rapid Fluorimetric Total Thiol Quantitation Assay Kit *Green Fluorescence*
Supplier: AAT BIOQUEST
The detection and measurement of free thiol (such as free cysteine, glutathione and cysteine residues in proteins) is one of the essential tasks for investigating biological processes and events in many biological systems.
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Amplite® Rapid Colorimetric Maleimide Quantitation Kit
Supplier: AAT BIOQUEST
A variety of crosslinking reagents with a maleimide group are widely used for crosslinking proteins to proteins, or proteins to other biomolecules.
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Amplite® Fluorimetric DPP4 Inhibitor Screening Kit
Supplier: AAT BIOQUEST
Designed for consistent and dependable results. The Amplite® Fluorimetric DPP4 Inhibitor Screening Kit provides an efficient and direct method for evaluating potential DPP4 inhibitors.
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Pierce™ Alkaline Phosphatase, Biotinylated
Supplier: Thermo Fisher Scientific
Biotinylated alkaline phosphatase is most commonly used in immunohistochemistry (IHC) to amplify the signal of biotinylated primary antibodies using the ABC staining method.
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Pierce™ Firefly Luc One-Step Glow Assay Kit
Supplier: Thermo Fisher Scientific
The Thermo Scientific Pierce Firefly One-Step Luc Glow Assay Kit measures firefly luciferase activity in mammalian cell culture using a single reagent-addition step, making it ideal for high-throughput screening (HTS) applications.
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Amplite® Human Serum Albumin (HSA) Site I Binding Assay Kit
Supplier: AAT BIOQUEST
Human serum albumin (HSA) is one of the most important carriers for acidic drugs in human plasma and has been shown to bind a large number of different compounds in a reversible manner.
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Amplite® Fluorimetric Acetylcholine Assay Kit *Red Fluorescence*
Supplier: AAT BIOQUEST
Acetylcholine and its metabolites are needed for three main physiological purposes: structural integrity and signaling roles for cell membranes, cholinergic neurotransmission (acetylcholine synthesis), and as a major source for methyl groups via its metabolite, trimethylglycine (betaine) that participates in the S-adenosylmethionine synthesis pathways.
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Amplite® Fluorimetric Coenzyme A Quantitation Kit *Green Fluorescence*
Supplier: AAT BIOQUEST
Coenzyme A (CoA) is a universal and essential cofactor in all forms of cellular life acting as a principal acyl carrier in numerous biosynthetic, energy-yielding, and degradative pathways.
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Amplite® Fluorimetric Fluorescamine Protein Quantitation Kit *Blue Fluorescence*
Supplier: AAT BIOQUEST
Fluorescamine is intrinsically non-fluorescent but reacts rapidly with primary aliphatic amines, including those in peptides and proteins, to yield a blue-green-fluorescent derivative.
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Screen Quest™ Fluorimetric Neutrophil Elastase Inhibitor Screening Kit
Supplier: AAT BIOQUEST
Designed for consistent and dependable results. The Screen Quest™ fluorimetric neutrophil elastase inhibitor screening kit offers a fast, sensitive, and high-throughput method for screening and characterising potential inhibitors of NE.
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β-Galactosidase Assay Kit and Reagents
Supplier: Thermo Fisher Scientific
The Mammalian β-Galactosidase Assay Reagent Kit is a simple and versatile assay to measure β-galactosidase activity in cultured mammalian cells. Cells grown on a plate may be lysed and tested for β-galactosidase activity by adding a single all-in-one lysis-and-assay solution. Alternatively, M-PER™ Mammalian Protein Extraction Reagent may be used to prepare lysate from washed adherent or pelleted cells, and then a portion of the lysate used to assay for β-galactosidase activity. With M-PER Reagent, there is no need to scrape cells or to perform freeze-thaw cycles. The remaining lysates prepared with M-PER™ Reagent may be used for applications such as reporter assays (luciferase, β-galactosidase and chloramphenical acetyltransferase), protein kinase assays (PKA, PKC, and tyrosine kinase), immunoassays (Western blot, ELISA, RIA) and protein purification.
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Amplite® Colorimetric Superoxide Dismutase (SOD) Assay Kit
Supplier: AAT BIOQUEST
Superoxide dismutases (SOD) are a class of enzymes that catalyse the dismutation of superoxide into oxygen and hydrogen peroxide.
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Amplite® Fluorimetric Melanin Assay Kit
Supplier: AAT BIOQUEST
Melanins have very diverse roles and functions in various organisms.
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Amplite® Luminometric Nitroreductase Assay Kit
Supplier: AAT BIOQUEST
Nitroreductases (NTR) are a family of evolutionarily related proteins involved in the reduction of nitrogen-containing compounds.
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Amplite® Colorimetric D-Lactate Assay Kit
Supplier: AAT BIOQUEST
Lactic acid is chiral and has two optical isomers: L-lactic acid and D-lactic acid.
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Amplite® Fluorimetric Ascorbic Acid Assay Kit
Supplier: AAT BIOQUEST
L-Ascorbic Acid (also called Vitamin C) is a critical metabolite for both plant and animals in cell division, growth and defense.
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Amplite® Colorimetric Beta-Hydroxybutyrate (Ketone Body) Assay Kit
Supplier: AAT BIOQUEST
Ketone bodies are produced by the liver and used peripherally as an energy source when blood glucose levels drop.
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Amplite® Colorimetric Fructose-6-Phosphate Activity Assay Kit
Supplier: AAT BIOQUEST
Designed for consistent and dependable results. The Amplite® colorimetric fructose-6-phosphate assay kit is a highly sensitive and simple method of quantifying F6P present in a variety of samples.
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Quantitative peroxide assay kit (aqueous), Pierce™
Supplier: Thermo Fisher Scientific
Pierce™ Quantitative Peroxide Assay Kit (Aqueous) detects and measures hydrogen peroxide levels in biological samples using an iron and xylenol orange* (XO) reagent.
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Amplite® Colorimetric Hypochlorite (Hypochlorous Acid) Assay Kit
Supplier: AAT BIOQUEST
Hypochlorite anion (ClO-) and its protonated form, hypochlorous acid (HClO) are critical reactive oxygen species (ROS) in biological systems.
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Amplite® Colorimetric Glycerol Assay Kit
Supplier: AAT BIOQUEST
Glycerol is a precursor for synthesis of triglycerides and phospholipids in the liver and adipose tissue.
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Amplite® Colorimetric BCA Protein Quantitation Assay Kit
Supplier: AAT BIOQUEST
The traditional BCA protein assay is widely used for quantifying proteins.
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Pierce™ Cypridina-Firefly Luciferase Dual Assay Kit
Supplier: Thermo Fisher Scientific
The Thermo Scientific Pierce Cypridina-Firefly Luciferase Dual Assay Kit provides the necessary reagents to simultaneously detect intracellular Cypridina and Red Firefly luciferase activity in mammalian whole cell lysates.
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Amplite® Colorimetric Glucose-6-Phosphate Assay Kit
Supplier: AAT BIOQUEST
Glucose-6-phosphate (G6P) is a key intermediate for glucose transport into cells.
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Amplite® Fluorimetric Caspase 3/7 Assay Kit *Blue Fluorescence*
Supplier: AAT BIOQUEST
Caspases play important roles in apoptosis and cell signaling.
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Phosphatase assay kit
Supplier: G-Biosciences
The Phosphatase Assay Kit is designed to measure the activity of phosphatases in biological samples and to screen for agonists and inhibitors of phosphatases. The Phosphatase Assay Kit uses para-nitrophenyl phosphate (pNPP), a chromogenic substrate for most phosphatases, including alkaline phosphatases, acid phosphatases, protein tyrosine phosphatases and serine/threonine phosphatases.
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Amplite® Fluorimetric L-Lactate Assay Kit
Supplier: AAT BIOQUEST
Lactic acid is chiral and has two optical isomers: L-lactic acid and D-lactic acid.
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Amplite® Fluorimetric Sphingomyelin Assay Kit *Red Fluorescence*
Supplier: AAT BIOQUEST
Sphingomyelin (SM) is largely found in the exoplasmic leaflet of the cell membrane, primarily in nervous tissue.
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Amplite® Colorimetric Glucose Quantitation Kit
Supplier: AAT BIOQUEST
Glucose, a monosaccharide, is the most important carbohydrate in biology.