Either measuring substrate consumption or overall production, the enzyme assays are used to monitor enzymatic activity. Provided with the required supplies and reagents for any preferred method, enzyme kinetics and inhibition studies can be successfully completed. While continuous assay sampling methods give activity readings throughout development, discontinuous techniques calculate after the reaction has been stopped. The standardized enzyme assays will provide accurate identification and measurement of the protein molecules present in samples.
Amplite® Colorimetric NADP/NADPH Ratio Assay Kit
Supplier: AAT BIOQUEST
Nicotinamide adenine dinucleotide (NAD+) and nicotinamide adenine dinucleotide phosphate (NADP+) are two important cofactors found in cells.
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Amplite® Fluorimetric Neutrophil Elastase Activity Assay Kit
Supplier: AAT BIOQUEST
Designed for consistent and dependable results. The Amplite® fluorimetric neutrophil elastase activity assay kit provides a robust and accurate method for quantifying neutrophil elastase (NE) activity.
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Amplite® Fluorimetric Hydrogen Peroxide Assay Kit *Red Fluorescence*
Supplier: AAT BIOQUEST
Hydrogen peroxide (H₂O₂) is a reactive oxygen metabolic by-product that serves as a key regulator for a number of oxidative stress-related states.
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Amplite® Fluorimetric Total NAD and NADH Assay Kit *Red Fluorescence*
Supplier: AAT BIOQUEST
Nicotinamide adenine dinucleotide (NAD+) and nicotinamide adenine dinucleotide phosphate (NADP+) are two important cofactors found in cells.
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Amplite® Colorimetric Ammonia Quantitation Kit *Blue Color*
Supplier: AAT BIOQUEST
Ammonia is an important source of nitrogen for living systems.
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Amplite® Colorimetric Urea Quantitation Kit *Blue Color*
Supplier: AAT BIOQUEST
Urea is the final degradation product of protein and amino acid metabolism in animals.
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Amplite® Colorimetric Zinc Ion Quantitation Kit
Supplier: AAT BIOQUEST
Zinc is an essential trace mineral element that plays an important role in a number of biological processes.
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Amplite® Colorimetric α-Ketoglutarate Quantitation Kit
Supplier: AAT BIOQUEST
Alpha-ketoglutarate (α-Ketoglutarate) is a key molecule in the Krebs cycle determining the overall rate of the citric acid cycle of the organism.
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Amplite® Fluorimetric Zinc Ion Quantitation Kit
Supplier: AAT BIOQUEST
Zinc is an essential trace mineral element that plays an important role in a number of biological processes.
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Amplite® Colorimetric Glucose and Sucrose Assay Kit
Supplier: AAT BIOQUEST
Designed for consistent and dependable results. The Amplite® Colorimetric Glucose and Sucrose Assay Kit offers a convenient method for measuring glucose and sucrose levels in various biological samples, such as serum, plasma, body fluids, food, tissue extracts, and growth medium.
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Amplite® Fluorimetric Total Thiol Quantitation Assay Kit *Green Fluorescence*
Supplier: AAT BIOQUEST
The detection and measurement of free thiol (such as free cysteine, glutathione and cysteine residues in proteins) is one of the essential tasks for investigating biological processes and events in many biological systems.
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Amplite® Rapid Fluorimetric Total Thiol Quantitation Assay Kit *Green Fluorescence*
Supplier: AAT BIOQUEST
The detection and measurement of free thiol (such as free cysteine, glutathione and cysteine residues in proteins) is one of the essential tasks for investigating biological processes and events in many biological systems.
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Amplite® Fluorimetric Coenzyme A Quantitation Kit *Green Fluorescence*
Supplier: AAT BIOQUEST
Coenzyme A (CoA) is a universal and essential cofactor in all forms of cellular life acting as a principal acyl carrier in numerous biosynthetic, energy-yielding, and degradative pathways.
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β-Galactosidase Assay Kit and Reagents
Supplier: Thermo Fisher Scientific
The Mammalian β-Galactosidase Assay Reagent Kit is a simple and versatile assay to measure β-galactosidase activity in cultured mammalian cells. Cells grown on a plate may be lysed and tested for β-galactosidase activity by adding a single all-in-one lysis-and-assay solution. Alternatively, M-PER™ Mammalian Protein Extraction Reagent may be used to prepare lysate from washed adherent or pelleted cells, and then a portion of the lysate used to assay for β-galactosidase activity. With M-PER Reagent, there is no need to scrape cells or to perform freeze-thaw cycles. The remaining lysates prepared with M-PER™ Reagent may be used for applications such as reporter assays (luciferase, β-galactosidase and chloramphenical acetyltransferase), protein kinase assays (PKA, PKC, and tyrosine kinase), immunoassays (Western blot, ELISA, RIA) and protein purification.
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Amplite® Colorimetric Beta-Lactamase Activity Assay Kit
Supplier: AAT BIOQUEST
β-lactamases are a large family of enzymes capable of hydrolysing β-lactams.
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Amplite® Fluorimetric D-Lactate Dehydrogenase (LDH) Assay Kit
Supplier: AAT BIOQUEST
Lactate dehydrogenase (LDH) is an oxidoreductase enzyme that catalyses the interconversion of pyruvate and lactate.
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Amplite® Fluorimetric Renin Assay Kit *Red Fluorescence*
Supplier: AAT BIOQUEST
Renin is an enzyme that participates in the body's renin-angiotensin system (RAS) that mediates extracellular volume and arterial vasoconstriction.
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Amplite® Fluorimetric Glutathione Peroxidase Assay Kit *Blue Fluorescence*
Supplier: AAT BIOQUEST
Glutathione peroxidase is an enzyme family with peroxidase activity to protect the organism from oxidative damage.
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Amplite® Fluorimetric Neuraminidase Assay Kit *Blue Fluorescence*
Supplier: AAT BIOQUEST
Neuraminidases, also called sialidases, are glycoside hydrolase enzymes that catalyze the hydrolysis of terminal sialic acid residues and neuraminic acid.
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Amplite® Fluorimetric Lysyl Oxidase Assay Kit *Red Fluorescence*
Supplier: AAT BIOQUEST
Lysyl oxidase (LOX) is an extracellular enzyme that catalyses formation of aldehydes from lysine residues in collagen and elastin precursors.
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Amplite® Colorimetric Enterokinase Activity Assay Kit
Supplier: AAT BIOQUEST
Enterokinase (also called enteropeptidase) is a serine protease produced by cells in the duodenal wall and is a key enzyme in human and animal digestion system.
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Amplite® Colorimetric Pyruvate Assay Kit
Supplier: AAT BIOQUEST
Pyruvate is an important chemical compound in intracellular metabolic pathways.
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Amplite® Colorimetric Maleimide Quantitation Kit
Supplier: AAT BIOQUEST
Maleimides can be directly assayed spectrophotometrically at 302 nm.
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Amplite® Colorimetric L-Alanine Assay Kit
Supplier: AAT BIOQUEST
L-alanine (L-Ala) plays a crucial role as a building block of important proteins.
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Amplite® Universal Fluorimetric MMP Activity Assay Kit *Green Fluorescence*
Supplier: AAT BIOQUEST
The matrix metalloproteinases (MMPs) constitute a family of zinc-dependent endopeptidases that function within the extracellular matrix.
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Amplite® Fluorimetric Oxaloacetate Assay Kit *Red Fluorescence*
Supplier: AAT BIOQUEST
Oxaloacetate is an important part of citric acid cycle, where it reacts with Acetyl-CoA to form citrate.
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Amplite® MMP-3 Activity Assay Kit *Green Fluorescence*
Supplier: AAT BIOQUEST
The matrix metalloproteinases (MMPs) constitute a family of zinc-dependent endopeptidases that function within the extracellular matrix.
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Amplite® Fluorimetric Asparaginase Activity Assay Kit
Supplier: AAT BIOQUEST
Designed for consistent and dependable results. The Amplite® Fluorimetric Asparaginase Activity Assay Kit provides a simple and straightforward procedure for measuring Asparaginase activity in a variety of biological samples.
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Amplite® Colorimetric Total NADP and NADPH Assay Kit
Supplier: AAT BIOQUEST
Nicotinamide adenine dinucleotide (NAD+) and nicotinamide adenine dinucleotide phosphate (NADP+) are two important cofactors found in cells.
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Renilla luciferase flash assay kit, Pierce™, Thermo Scientific
Supplier: Thermo Fisher Scientific
The Thermo Scientific Pierce Renilla Luciferase Flash Assay Kit provides researchers with a highly sensitive intracellular assay for transcriptional activity of regulatory elements in mammalian whole cell lysate.