You searched for: Enzyme Assays
Either measuring substrate consumption or overall production, the enzyme assays are used to monitor enzymatic activity. Provided with the required supplies and reagents for any preferred method, enzyme kinetics and inhibition studies can be successfully completed. While continuous assay sampling methods give activity readings throughout development, discontinuous techniques calculate after the reaction has been stopped. The standardized enzyme assays will provide accurate identification and measurement of the protein molecules present in samples.
Protease and peptidase substrate
Supplier: Biotium
Fluorogenic substrates for proteases and peptidases.
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PKA kinase activity kit
Supplier: ENZO LIFE SCIENCES
The PKA Kinase activity kit is an efficient assay that utilizes little sample volume. This kit gives you the ability to an end-point or kinetic assay read-out in a convenient 96-well plate based assay and offers easy sample handling protocols. The assay also offers a high signal to background ratio.
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Matrix Metalloproteinase-1 (MMP-1) colorimetric drug discovery kit
Supplier: ENZO LIFE SCIENCES
A QuantiZyme™ Assay System. The MMP-1 Colorimetric Drug Discovery Kit is a complete assay system designed to screen inhibitors of matrix metalloproteinase-1 (MMP-1, interstitial collagenase, fibroblast collagenase) using a thiopeptide as a chromogenic substrate.
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Matrix Metalloproteinase-7 (MMP-7) fluorometric drug discovery kit
Supplier: ENZO LIFE SCIENCES
A QuantiZyme™ Assay System. The MMP-7 Fluorometric (also known as fluorimetric) Drug Discovery Kit is a complete assay system designed to screen inhibitors of matrix metalloproteinase-7 (MMP-7, matrilysin) using a quenched fluorogenic peptide: OmniMMP™ fluorogenic substrate Mca-Pro-Leu-Gly-Leu-Dpa-Ala-Arg-NH. Mca fluorescence is quenched by the Dpa group until cleavage by MMPs at the Gly-Leu bond separates the two moieties.
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Matrix Metalloproteinase-13 (MMP-13) fluorometric drug discovery kit
Supplier: ENZO LIFE SCIENCES
A QuantiZyme™ Assay System. The MMP-13 Fluorometric (also known as fluorimetric) Drug Discovery Kit is a complete assay system designed to screen inhibitors of matrix metalloproteinase-13 (MMP-13, collagenase-3) using a quenched fluorogenic peptide: OmniMMP™ fluorogenic substrate Mca-Pro-Leu-Gly-Leu-Dpa-Ala-Arg-NH. Mca fluorescence is quenched by the Dpa group until cleavage by MMPs at the Gly-Leu bond separates the two moieties.
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Sclerostin-LRP interaction screening system, LEADING LIGHT®
Supplier: ENZO LIFE SCIENCES
Novel Cell-free Sclerostin/LRP Binding Assay Ideal for Primary Inhibitor Screens.
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Arginase I (ARG1) Inhibitor Screening Kit (Colorimetric)
Supplier: Abcam
The most sensitive kit to evaluate potential human Arginase I inhibitors.
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Matrix Metalloproteinase-2 (MMP-2) colorimetric drug discovery kit
Supplier: ENZO LIFE SCIENCES
A QuantiZyme™ Assay System. The MMP-2 Colorimetric Drug Discovery Kit is a complete assay system designed to screen inhibitors of matrix metalloproteinase-2 (MMP-2, gelatinase A) using a thiopeptide as a chromogenic substrate. The assays are performed in a convenient 96-well microplate format. The kit is useful to screen inhibitors of MMP-2, a potential therapeutic target.
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Matrix Metalloproteinase-10 (MMP-10) fluorometric drug discovery kit
Supplier: ENZO LIFE SCIENCES
A QuantiZyme™ Assay System. The MMP-10 Fluorometric (also known as fluorimetric) Drug Discovery Kit is a complete assay sytem designed to screen for inhibitors of matrix metalloproteinase-10 (MMP-10, stromelysin-2) using a quenched fluorogenic peptide: OmniMMP™ fluorogenic substrate Mca-Pro-Leu-Gly-Leu-Dpa-Ala-Arg-NH. Mca fluorescence is quenched by the Dpa group until cleavage by MMPs at the Gly-Leu bond separates the two moieties.
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SAM Methyltransferase assay, SAMfluoro™
Supplier: G-Biosciences
The SAMfluoro™ assay is a fluorescent, continuous enzyme coupled assay that can continuously monitor SAM-dependent methyltransferases without the use of radioactive labels or endpoint measurements.
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Amplite® Colorimetric Phosphofructokinase (PFK) Activity Assay Kit
Supplier: AAT BIOQUEST
Designed for consistent and dependable results. The Amplite® Colorimetric Phosphofructokinase (PFK) Activity Assay Kit provides a simple and direct procedure for precise measurement of PFK enzyme activity in various biological samples.
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Amplite® Universal Fluorimetric Kinase Assay Kit *Red Fluorescence*
Supplier: AAT BIOQUEST
The Amplite® Universal Kinase Assay Kit is based on the monitoring of ADP formation, which is directly proportional to enzyme phoshphotransferase activity and is measured fluorimetricaly. This kit provides a fast, simple, and homogeneous assay for measure kinases activities. The characteristics of its high sensitivity (<0.2 uM ADP), broad ATP tolerance (1-300 uM), non-antibody based, non-radioactive and no-wash method to detect the amount of ADP produced as a result of enzyme activity make it an ideal kit for determining kinase Michaelis-Menten kinetics and for screening and identifying kinase inhibitors. The assay can be performed in a convenient 96-well or 384-well microtiter-plate format and easily adapted to automation with no separation steps required.
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Amplite® Colorimetric Beta-Lactamase Activity Assay Kit
Supplier: AAT BIOQUEST
β-lactamases are a large family of enzymes capable of hydrolysing β-lactams.
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Amplite® Fluorimetric D-Lactate Dehydrogenase (LDH) Assay Kit
Supplier: AAT BIOQUEST
Lactate dehydrogenase (LDH) is an oxidoreductase enzyme that catalyses the interconversion of pyruvate and lactate.
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Amplite® Fluorimetric Renin Assay Kit *Red Fluorescence*
Supplier: AAT BIOQUEST
Renin is an enzyme that participates in the body's renin-angiotensin system (RAS) that mediates extracellular volume and arterial vasoconstriction.
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Amplite® Fluorimetric Glutathione Peroxidase Assay Kit *Blue Fluorescence*
Supplier: AAT BIOQUEST
Glutathione peroxidase is an enzyme family with peroxidase activity to protect the organism from oxidative damage.
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Amplite® Fluorimetric Neuraminidase Assay Kit *Blue Fluorescence*
Supplier: AAT BIOQUEST
Neuraminidases, also called sialidases, are glycoside hydrolase enzymes that catalyze the hydrolysis of terminal sialic acid residues and neuraminic acid.
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Amplite® Fluorimetric Lysyl Oxidase Assay Kit *Red Fluorescence*
Supplier: AAT BIOQUEST
Lysyl oxidase (LOX) is an extracellular enzyme that catalyses formation of aldehydes from lysine residues in collagen and elastin precursors.
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Amplite® Colorimetric Enterokinase Activity Assay Kit
Supplier: AAT BIOQUEST
Enterokinase (also called enteropeptidase) is a serine protease produced by cells in the duodenal wall and is a key enzyme in human and animal digestion system.
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Amplite® Colorimetric Pyruvate Assay Kit
Supplier: AAT BIOQUEST
Pyruvate is an important chemical compound in intracellular metabolic pathways.
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Amplite® Colorimetric Maleimide Quantitation Kit
Supplier: AAT BIOQUEST
Maleimides can be directly assayed spectrophotometrically at 302 nm.
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Amplite® Colorimetric L-Alanine Assay Kit
Supplier: AAT BIOQUEST
L-alanine (L-Ala) plays a crucial role as a building block of important proteins.
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Amplite® Universal Fluorimetric MMP Activity Assay Kit *Green Fluorescence*
Supplier: AAT BIOQUEST
The matrix metalloproteinases (MMPs) constitute a family of zinc-dependent endopeptidases that function within the extracellular matrix.
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Amplite® Fluorimetric Oxaloacetate Assay Kit *Red Fluorescence*
Supplier: AAT BIOQUEST
Oxaloacetate is an important part of citric acid cycle, where it reacts with Acetyl-CoA to form citrate.
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Amplite® MMP-3 Activity Assay Kit *Green Fluorescence*
Supplier: AAT BIOQUEST
The matrix metalloproteinases (MMPs) constitute a family of zinc-dependent endopeptidases that function within the extracellular matrix.
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Amplite® Fluorimetric Asparaginase Activity Assay Kit
Supplier: AAT BIOQUEST
Designed for consistent and dependable results. The Amplite® Fluorimetric Asparaginase Activity Assay Kit provides a simple and straightforward procedure for measuring Asparaginase activity in a variety of biological samples.
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Amplite® Colorimetric Total NADP and NADPH Assay Kit
Supplier: AAT BIOQUEST
Nicotinamide adenine dinucleotide (NAD+) and nicotinamide adenine dinucleotide phosphate (NADP+) are two important cofactors found in cells.
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Renilla luciferase flash assay kit, Pierce™, Thermo Scientific
Supplier: Thermo Fisher Scientific
The Thermo Scientific Pierce Renilla Luciferase Flash Assay Kit provides researchers with a highly sensitive intracellular assay for transcriptional activity of regulatory elements in mammalian whole cell lysate.
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Cypridina luciferase flash assay kit, Pierce™
Supplier: Thermo Fisher Scientific
The Thermo Scientific Pierce Cypridina Luciferase Flash Assay Kit provides researchers with a highly sensitive assay for transcriptional activity of regulatory elements in mammalian cell culture media and whole cell lysate.
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Quantitative peroxide assay kit (lipid), Pierce™
Supplier: Thermo Fisher Scientific
Pierce™ Quantitative Peroxide Assay Kit (Lipid) detects and measures hydrogen peroxide levels in biological samples using an iron and xylenol orange* (XO) reagent.