Ihre Suche nach: Enzyme Assays
Either measuring substrate consumption or overall production, the enzyme assays are used to monitor enzymatic activity. Provided with the required supplies and reagents for any preferred method, enzyme kinetics and inhibition studies can be successfully completed. While continuous assay sampling methods give activity readings throughout development, discontinuous techniques calculate after the reaction has been stopped. The standardized enzyme assays will provide accurate identification and measurement of the protein molecules present in samples.
PTP1B drug discovery kit
Supplier: ENZO LIFE SCIENCES
The PTP1B Tyrosine Phosphatase Drug Discovery Kit is a colorimetric, non-radioactive assay designed to measure the phosphatase activity of purified PTP1B. This 96-well assay is useful for screening inhibitors and modulators of PTP1B activity.
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Cathepsin S drug discovery kit
Supplier: ENZO LIFE SCIENCES
A QuantiZyme™ Assay System. The Cathepsin S Drug Discovery Kit is a complete assay system designed to screen inhibitors of cathepsin S. Using a convenient microplate format, cathepsin S activity is monitored fluorimetrically using the substrate provided in the kit.
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Glutathione peroxidase activity kit
Supplier: ENZO LIFE SCIENCES
The Glutathione Peroxidase activity kit is a convenient 96-well plate based assay for the higher throughput analysis of glutathione peroxidase activity in a variety of sample matrices in just 30 minutes.
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Fluorometric drug discovery assay kit, Green SIRT5, Fluor-de-Lys®
Supplier: ENZO LIFE SCIENCES
FLUOR DE LYS® Green SIRT5 fluorometric drug discovery kit is a sensitive fluorometric assay that measures the lysyl desuccinylase activity of the recombinant human SIRT5.
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Matrix Metalloproteinase-13 (MMP-13) colorimetric drug discovery kit
Supplier: ENZO LIFE SCIENCES
A QuantiZyme™ Assay System. The MMP-13 Colorimetric Drug Discovery Kit is a complete assay system designed to screen inhibitors of matrix metalloproteinase-13 (MMP-13, collagenase-3) using a thiopeptide as a chromogenic substrate.
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Matrix Metalloproteinase-20 (MMP-20) colorimetric drug discovery kit
Supplier: ENZO LIFE SCIENCES
A QuantiZyme™ Assay System. The MMP-20 Colorimetric Drug Discovery Kit is a complete assay system designed to screen inhibitors of matrix metalloproteinase-20 (MMP-20, enamelysin) using a thiopeptide as a chromogenic substrate.
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Protease assay kit
Supplier: G-Biosciences
This Protease Assay Kit is designed for the quantitative determination of proteases present in protein sample, using a dye-labelled protein substrate. The proteases present in the sample of interest will digest the protein substrate and release dye labelled peptides. The absorbance of the dye-labelled peptide is measured at 570 nm for determination of protease activity.
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Carbonic Anhydrase (CA) Inhibitor Screening Kit (Colorimetric)
Supplier: Abcam
It is simple and can be used to identify and characterize CA inhibitors in a high-throughput format.
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Matrix Metalloproteinase-14 (MMP-14) fluorometric drug discovery kit
Supplier: ENZO LIFE SCIENCES
A QuantiZyme™ Assay System. The MMP-14 Fluorometric (also known as fluorimetric) Drug Discovery Kit is a complete assay sytem designed to screen for inhibitors of matrix metalloproteinase-14 (MMP-14, membrane-type matrix metalloproteinase 1, MT1-MMP) using a quenched fluorogenic peptide: OmniMMP™ fluorogenic substrate Mca-Pro-Leu-Gly-Leu-Dpa-Ala-Arg-NH. Mca fluorescence is quenched by the Dpa group until cleavage by MMPs at the Gly-Leu bond separates the two moieties.
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SAM Methyltransferase assay, SAMfluoro™
Supplier: G-Biosciences
The SAMfluoro™ assay is a fluorescent, continuous enzyme coupled assay that can continuously monitor SAM-dependent methyltransferases without the use of radioactive labels or endpoint measurements.
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Amplite® Universal Fluorimetric Kinase Assay Kit *Red Fluorescence*
Supplier: AAT BIOQUEST
The Amplite® Universal Kinase Assay Kit is based on the monitoring of ADP formation, which is directly proportional to enzyme phoshphotransferase activity and is measured fluorimetricaly. This kit provides a fast, simple, and homogeneous assay for measure kinases activities. The characteristics of its high sensitivity (<0.2 uM ADP), broad ATP tolerance (1-300 uM), non-antibody based, non-radioactive and no-wash method to detect the amount of ADP produced as a result of enzyme activity make it an ideal kit for determining kinase Michaelis-Menten kinetics and for screening and identifying kinase inhibitors. The assay can be performed in a convenient 96-well or 384-well microtiter-plate format and easily adapted to automation with no separation steps required.
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Amplite® Colorimetric Phosphofructokinase (PFK) Activity Assay Kit
Supplier: AAT BIOQUEST
Designed for consistent and dependable results. The Amplite® Colorimetric Phosphofructokinase (PFK) Activity Assay Kit provides a simple and direct procedure for precise measurement of PFK enzyme activity in various biological samples.
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Amplite® Fluorimetric Acidic Sphingomyelinase Assay Kit *Red Fluorescence*
Supplier: AAT BIOQUEST
Sphingomyelinase (SMase) is an enzyme that is responsible for cleaving sphingomyelin (SM) to phosphocholine and ceramide.
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Amplite® Colorimetric D-Lactate Dehydrogenase (LDH) Assay Kit
Supplier: AAT BIOQUEST
Lactate dehydrogenase (LDH) is an oxidoreductase enzyme that catalyses the interconversion of pyruvate and lactate.
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Amplite® Colorimetric Beta-Galactosidase Assay Kit
Supplier: AAT BIOQUEST
E. coli beta-galactosidase is a 464 kD tetramer.
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Amplite® Fluorimetric NADP Assay Kit *Blue Fluorescence*
Supplier: AAT BIOQUEST
Nicotinamide adenine dinucleotide (NAD+) and nicotinamide adenine dinucleotide phosphate (NADP+) are two important cofactors for many enzyme reactions found in living cells.
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Amplite® Colorimetric Caspase 3/7 Assay Kit *Yellow Color*
Supplier: AAT BIOQUEST
Caspases play important roles in apoptosis and cell signaling.
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Amplite® Fluorimetric Glutamic Acid Assay Kit *Red Fluorescence*
Supplier: AAT BIOQUEST
Glutamic acid is one of the 20 proteinogenic amino acids.
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Amplite® Luminometric Peroxidase (HRP) Assay Kit
Supplier: AAT BIOQUEST
Peroxidase is a small molecule (MW ~40 KD) that can usually be conjugated to an antibody in a 4:1 ratio.
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Amplite® Colorimetric L-Lactate Assay Kit
Supplier: AAT BIOQUEST
Lactic acid is chiral and has two optical isomers: L-lactic acid and D-lactic acid.
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Amplite® Fluorimetric Peroxidase (HRP) Assay Kit *Red Fluorescence*
Supplier: AAT BIOQUEST
Peroxidase is a small molecule (MW ~40 KD) that can usually be conjugated to an antibody in a 4:1 ratio.
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Amplite® Colorimetric Antioxidant Activity Assay Kit
Supplier: AAT BIOQUEST
Reactive oxygen species (ROS) are essential in cell signaling and maintaining physiological cell metabolism.
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Amplite® Colorimetric Lipase Activity Assay Kit
Supplier: AAT BIOQUEST
Designed for consistent and dependable results. The Amplite® colorimetric lipase activity assay kit offers a straightforward and efficient protocol for quantifying lipase activity.
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Amplite® Universal Fluorimetric MMP Activity Assay Kit *Red Fluorescence*
Supplier: AAT BIOQUEST
The matrix metalloproteinases (MMPs) constitute a family of zinc-dependent endopeptidases that function within the extracellular matrix.
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Amplite® Total Sulfide Quantification Kit
Supplier: AAT BIOQUEST
Hydrogen sulfide is not usually a health risk at concentrations present in household water.
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Amplite® Fluorimetric Glucose Quantitation Kit
Supplier: AAT BIOQUEST
Glucose, a monosaccharide, is the most important carbohydrate in biology.
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Amplite® Colorimetric Tyrosinase Assay Kit
Supplier: AAT BIOQUEST
Tyrosinase is of great interest to drug discovery, life science research, food industry and cosmetics industry since it plays an important role in the biosynthetic pathway of melanin.
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Amplite® Fluorimetric NADP/NADPH Ratio Assay Kit *Red Fluorescence*
Supplier: AAT BIOQUEST
Nicotinamide adenine dinucleotide (NAD⁺) and nicotinamide adenine dinucleotide phosphate (NADP⁺) are two important cofactors found in cells.
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Amplite® IR
Supplier: AAT BIOQUEST
Our Amplite® IR is a fluorogenic peroxidase substrate that generates near infrared fluorescence upon reaction with peroxidase and H2O2.
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Amplite® Fluorimetric Malondialdehyde (MDA) Quantitation Kit
Supplier: AAT BIOQUEST
Malondialdehyde (MDA) is one of the natural byproducts during lipid peroxidation.