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Ihre Suche nach: Enzyme Assays

Either measuring substrate consumption or overall production, the enzyme assays are used to monitor enzymatic activity. Provided with the required supplies and reagents for any preferred method, enzyme kinetics and inhibition studies can be successfully completed. While continuous assay sampling methods give activity readings throughout development, discontinuous techniques calculate after the reaction has been stopped. The standardized enzyme assays will provide accurate identification and measurement of the protein molecules present in samples.

Matrix Metalloproteinase-3 (MMP-3) fluorometric drug discovery kit

Supplier: ENZO LIFE SCIENCES

A QuantiZyme™ Assay System. The MMP-3 Fluorometric (also known as fluorimetric) Drug Discovery Kit is a complete assay system designed to screen inhibitors of matrix metalloproteinase-3 (MMP-3, stromelysin-1) using a quenched fluorogenic peptide: OmniMMP™ fluorogenic substrate Mca-Pro-Leu-Gly-Leu-Dpa-Ala-Arg-NH. Mca fluorescence is quenched by the Dpa group until cleavage by MMPs at the Gly-Leu bond separates the two moieties.

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Fluorescent protease assay, Fluoro™

Fluorescent protease assay, Fluoro™

Supplier: G-Biosciences

The Fluoro™ Protease Assay Kit is designed for the quantitative determination of proteases present in a protein sample. The assay uses fluorescein isothiocyanate (FITC)-labelled casein as a general protease substrate. The fluorescein label on the FITC-casein is highly quenched. When the proteases present in the sample of interest digest the FITC-casein substrate into smaller peptides, the quenching of the fluorescence label is relieved and the fluorescence of the substrate is increased. The fluorescence of the FITC-labeled peptide is measured with excitation at 485 nm and emission at 530 nm to determine protease activity. The kit detects picogram level of proteases present in the sample.

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Matrix Metalloproteinase-9 (MMP-9) fluorometric drug discovery kit

Supplier: ENZO LIFE SCIENCES

A QuantiZyme™ Assay SystemThe MMP-9 Fluorometric (also known as fluorimetric) Drug Discovery Kit is a complete assay system designed to screen inhibitors of matrix metalloproteinase-9 (MMP-9, gelatinase B) using a quenched fluorogenic peptide: OmniMMP™ fluorogenic substrate Mca-Pro-Leu-Gly-Leu-Dpa-Ala-Arg-NH. Mca fluorescence is quenched by the Dpa group until cleavage by MMPs at the Gly-Leu bond separates the two moieties.

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AEC Peroxidase substrate kit

Supplier: ENZO LIFE SCIENCES

The AEC Peroxidase Substrate Kit contains reagents for visualization of peroxidase reactions using Aminoethylcarbazole (AEC). In the presence of H2O2, peroxidases catalyze the oxidation of AEC into a brick red precipitate. The supplied reagents are sufficient for preparation of 300 ml of ready-to-use color reaction mixture.

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Cyclic ucleotide phosphodiesterase assay kit components

Supplier: ENZO LIFE SCIENCES

3', 5'-cGMP substrate

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Creatinine (urinary) colorimetric assay kit

Supplier: Cayman Chemical

For the measurement of creatinine levels in urine.

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IMAP kinase, phosphatase, and phosphodiesterase assays, molecular devices

Supplier: Molecular Devices

IMAP® technology provides a homogeneous assay applicable to a wide variety of kinases without regard for substrate peptide sequence.

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PTP1B drug discovery kit

Supplier: ENZO LIFE SCIENCES

The PTP1B Tyrosine Phosphatase Drug Discovery Kit is a colorimetric, non-radioactive assay designed to measure the phosphatase activity of purified PTP1B. This 96-well assay is useful for screening inhibitors and modulators of PTP1B activity.

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Cathepsin S drug discovery kit

Supplier: ENZO LIFE SCIENCES

A QuantiZyme™ Assay System. The Cathepsin S Drug Discovery Kit is a complete assay system designed to screen inhibitors of cathepsin S. Using a convenient microplate format, cathepsin S activity is monitored fluorimetrically using the substrate provided in the kit.

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Glutathione peroxidase activity kit

Supplier: ENZO LIFE SCIENCES

The Glutathione Peroxidase activity kit is a convenient 96-well plate based assay for the higher throughput analysis of glutathione peroxidase activity in a variety of sample matrices in just 30 minutes.

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Fluorometric drug discovery assay kit, Green SIRT5, Fluor-de-Lys®

Fluorometric drug discovery assay kit, Green SIRT5, Fluor-de-Lys®

Supplier: ENZO LIFE SCIENCES

FLUOR DE LYS® Green SIRT5 fluorometric drug discovery kit is a sensitive fluorometric assay that measures the lysyl desuccinylase activity of the recombinant human SIRT5.

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Matrix Metalloproteinase-13 (MMP-13) colorimetric drug discovery kit

Supplier: ENZO LIFE SCIENCES

A QuantiZyme™ Assay System. The MMP-13 Colorimetric Drug Discovery Kit is a complete assay system designed to screen inhibitors of matrix metalloproteinase-13 (MMP-13, collagenase-3) using a thiopeptide as a chromogenic substrate.

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Matrix Metalloproteinase-20 (MMP-20) colorimetric drug discovery kit

Supplier: ENZO LIFE SCIENCES

A QuantiZyme™ Assay System. The MMP-20 Colorimetric Drug Discovery Kit is a complete assay system designed to screen inhibitors of matrix metalloproteinase-20 (MMP-20, enamelysin) using a thiopeptide as a chromogenic substrate.

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Protease assay kit

Protease assay kit

Supplier: G-Biosciences

This Protease Assay Kit is designed for the quantitative determination of proteases present in protein sample, using a dye-labelled protein substrate. The proteases present in the sample of interest will digest the protein substrate and release dye labelled peptides. The absorbance of the dye-labelled peptide is measured at 570 nm for determination of protease activity.

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Carbonic Anhydrase (CA) Inhibitor Screening Kit (Colorimetric)

Carbonic Anhydrase (CA) Inhibitor Screening Kit (Colorimetric)

Supplier: Abcam

It is simple and can be used to identify and characterize CA inhibitors in a high-throughput format.

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Matrix Metalloproteinase-14 (MMP-14) fluorometric drug discovery kit

Supplier: ENZO LIFE SCIENCES

A QuantiZyme™ Assay System. The MMP-14 Fluorometric (also known as fluorimetric) Drug Discovery Kit is a complete assay sytem designed to screen for inhibitors of matrix metalloproteinase-14 (MMP-14, membrane-type matrix metalloproteinase 1, MT1-MMP) using a quenched fluorogenic peptide: OmniMMP™ fluorogenic substrate Mca-Pro-Leu-Gly-Leu-Dpa-Ala-Arg-NH. Mca fluorescence is quenched by the Dpa group until cleavage by MMPs at the Gly-Leu bond separates the two moieties.

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Caspase-3 fluorometric assay kit

Supplier: ENZO LIFE SCIENCES

Based on the detection of AFC (7-amino-4-trifluoromethyl coumarin) after cleavage from the labelled substrate DEVD-AFC.

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Superoxide dismutase assay kit

Superoxide dismutase assay kit

Supplier: Cayman Chemical

Superoxide Dismutase Assay (SOD) kit is a fast and reliable assay for the measurement of SOD activity from plasma, serum, tissue homogenates and cell lysates.

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Proteasome 20S Assay Kit

Supplier: ENZO LIFE SCIENCES

A QuantiZyme™ Assay System. Fluorogenic, non-radioactive assay designed to measure chymotrypsin-like protease activity of purified 20S proteasome. This 96-well assay is useful for screening inhibitors and modulators of the 20S proteasome.

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Protease and peptidase substrate

Supplier: Biotium

Fluorogenic substrates for proteases and peptidases.

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PKA kinase activity kit

Supplier: ENZO LIFE SCIENCES

The PKA Kinase activity kit is an efficient assay that utilizes little sample volume. This kit gives you the ability to an end-point or kinetic assay read-out in a convenient 96-well plate based assay and offers easy sample handling protocols. The assay also offers a high signal to background ratio.

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Matrix Metalloproteinase-1 (MMP-1) colorimetric drug discovery kit

Matrix Metalloproteinase-1 (MMP-1) colorimetric drug discovery kit

Supplier: ENZO LIFE SCIENCES

A QuantiZyme™ Assay System. The MMP-1 Colorimetric Drug Discovery Kit is a complete assay system designed to screen inhibitors of matrix metalloproteinase-1 (MMP-1, interstitial collagenase, fibroblast collagenase) using a thiopeptide as a chromogenic substrate.

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Neutrophil Elastase colorimetric drug discovery kit

Neutrophil Elastase colorimetric drug discovery kit

Supplier: ENZO LIFE SCIENCES

Neutrophil Elastase Colorimetric Drug Discovery Kit is a complete assay system designed to screen inhibitors of neutrophil elastase, providing enough material to perform at least 96 assays.

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PKC kinase activity kit

Supplier: ENZO LIFE SCIENCES

The PKC Kinase activity kit is an efficient assay that utilizes little sample volume. This kit gives you the ability to an end-point or kinetic assay read-out in a convenient 96-well plate based assay and offers easy sample handling protocols. The assay also offers a high signal to background ratio.

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Phosphatase assay kit, BIOMOL® Green

Phosphatase assay kit, BIOMOL® Green

Supplier: ENZO LIFE SCIENCES

BIOMOL® Green provides a simple and convenient method for colorimetric phosphate quantitation. It is suitable for applications include assays for phospholipid phosphatases, tyrosyl-tRNA synthetase (coupled with pyrophosphatase) and a viral RNA triphosphatase. It is most widely applied to protein phosphatase assays.

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ProteoStat™ PDI assay kit

Supplier: ENZO LIFE SCIENCES

The PROTEOSTAT® PDI assay kit provides a simple, homogenous assay for screening modulators of PDI enzymatic activity. This is accomplished by monitoring PDI-catalyzed reduction of insulin in the presence of Dithiothreitol (DTT), resulting in the formation of insulin aggregates which then bind avidly to the red-emitting fluorogenic PROTEOSTAT® PDI detection dye.

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Matrix Metalloproteinase-7 (MMP-7) fluorometric drug discovery kit

Supplier: ENZO LIFE SCIENCES

A QuantiZyme™ Assay System. The MMP-7 Fluorometric (also known as fluorimetric) Drug Discovery Kit is a complete assay system designed to screen inhibitors of matrix metalloproteinase-7 (MMP-7, matrilysin) using a quenched fluorogenic peptide: OmniMMP™ fluorogenic substrate Mca-Pro-Leu-Gly-Leu-Dpa-Ala-Arg-NH. Mca fluorescence is quenched by the Dpa group until cleavage by MMPs at the Gly-Leu bond separates the two moieties.

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Matrix Metalloproteinase-13 (MMP-13) fluorometric drug discovery kit

Supplier: ENZO LIFE SCIENCES

A QuantiZyme™ Assay System. The MMP-13 Fluorometric (also known as fluorimetric) Drug Discovery Kit is a complete assay system designed to screen inhibitors of matrix metalloproteinase-13 (MMP-13, collagenase-3) using a quenched fluorogenic peptide: OmniMMP™ fluorogenic substrate Mca-Pro-Leu-Gly-Leu-Dpa-Ala-Arg-NH. Mca fluorescence is quenched by the Dpa group until cleavage by MMPs at the Gly-Leu bond separates the two moieties.

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Neutrophil Elastase Activity Assay Kit

Neutrophil Elastase Activity Assay Kit

Supplier: Cayman Chemical

A convenient all-in-one tool to measure neutrophil elastase activity.

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Fluorometric polymerase activity assay kit, EvaEZ™

Supplier: Biotium

EvaEZ™ Fluorometric Polymerase Activity Assay Kit provides an easy and accurate way to determine the activity of a nucleic acid polymerase without using radioisotopes.

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