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Cellular Assays

Cellular assays have always been a powerful tool in the research lab. They are incredibly versatile, and can be designed to measure virtually any cellular or biochemical function. Sensitive detection of fluorescent probes aid in evaluating cell signaling and death pathways, which is useful for the rapid quantification of cell viability. Cellular assays can be used to measure the effects of different treatments on cell viability and to evaluate toxic effects mediated through a variety of mechanisms.

LysoView™ Lysosomal Stain

LysoView™ Lysosomal Stain

Supplier: Biotium

LysoView™ dyes label lysosomes in live cells, and are available with blue, green, visible red, and far-red fluorescence.

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Cell proliferation assay kit, WST-1

Supplier: Cayman Chemical

WST-1 Proliferation Assay Kit provides an easy to use tool for studying the induction and inhibition of cell proliferation in any in vitro model. The assay is based on the reduction of tetrazolium salt WST-1 to soluble formazan by electron transport across the plasma membrane of dividing cells. This kit will also allow investigators to screen drug candidates involved in regulation of cell cycle.

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Aggresome detection kit, PROTEOSTAT®

Supplier: ENZO LIFE SCIENCES

PROTEOSTAT® Aggresome Detection Kit is a robust, quantitative detection of aggresomes relevant to the study of neurodegenerative disease, liver disease and toxicology.

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Calcein-AM cell viability assay kits

Calcein-AM cell viability assay kits

Supplier: Biotium

Calcein-AM is a non-fluorescent, membrane permeable compound. Esterase activity in the cytoplasm of viable cells converts calcein-AM to the green fluorescent, membrane impermeant compound calcein, which is retained in viable cells with intact plasma membranes. The Viability/Cytotoxicity Assay kit for Animal Live & Dead Cells pairs calcein-AM with the vital dye ethidium homodimer III for quantitation of live and dead cells.

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PPARα transcription factor assay kit

PPARα transcription factor assay kit

Supplier: Cayman Chemical

A 96-well assay for measurement of PPARα DNA binding activity.

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Apoptosis assay kit, NucView® 488 and MitoView™ 633

Apoptosis assay kit, NucView® 488 and MitoView™ 633

Supplier: Biotium

NucView® 488 and MitoView™ 633 kit allows profiling of apoptotic cells simultaneously with changes in mitochondrial membrane potential using either fluorescence microscopy or flow cytometry.

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Cell Meter™ Fluorimetric Cellular Lipid Peroxidation Assay Kit

Cell Meter™ Fluorimetric Cellular Lipid Peroxidation Assay Kit

Supplier: AAT BIOQUEST

Lipid peroxidation is the oxidative degradation of cellular lipid by reactive oxygen species (ROS).

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p53 transcription factor assay kit

p53 transcription factor assay kit

Supplier: Cayman Chemical

96-Well assay for measurement of p53.

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Live bacterial gram stain kit

Supplier: Biotium

Wheat germ agglutinin binds specifically to N-acetylglucosamine in the peptidoglycan layer of gram-positive bacteria. The Live Bacterial Gram Stain Kit contains wheat germ agglutinin (WGA) conjugated to CF™594 to stain the surface of gram-positive cells with red fluorescence. The kit also includes DAPI to stain the DNA of all bacteria with blue fluorescence. Gram-positive bacteria stained using this kit will show a fluorescent blue interior and fluorescent red cell surface, while gram-negative bacterial will be stained with blue fluorescence only.

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PPARδ transcription factor assay kit

PPARδ transcription factor assay kit

Supplier: Cayman Chemical

A 96-well assay for measurement of PPARδ DNA binding activity.

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Green detection kit, MITO-ID®

Supplier: ENZO LIFE SCIENCES

MITO-ID® Green Detection Kit is a potent mitochondria-selective dye suitable for live, permeabilised and fixed cell staining.

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GFP-CERTIFIED® FLUOFORTE® Calcium assay kit

Supplier: ENZO LIFE SCIENCES

The GFP-Certified™ FluoForte™ Calcium Assay kit provides a homogeneous fluorescence-based assay for detecting intracellular calcium mobilization across a broad spectrum of biological targets.

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HIF-1α transcription factor assay Kit

HIF-1α transcription factor assay Kit

Supplier: Cayman Chemical

HIF-1α Transcription Factor Assay Kit is a sensitive, non radioactive method for detecting HIF-1α from whole cell lysates.

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CV-Cathepsin B detection kit

Supplier: ENZO LIFE SCIENCES

Based on the dye cresyl violet and the cathepsin B ArgArg peptide recognition sequence, this kit allows the detection of cathepsin B activity in living cells by fluorescence microscopy.

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Cell Meter™ Cellular Senescence Activity Assay Kit *Red Fluorescence*

Cell Meter™ Cellular Senescence Activity Assay Kit *Red Fluorescence*

Supplier: AAT BIOQUEST

Cellular Senescence is an irreversible growth arrest triggered in order to prevent growth in DNA damaged cells.

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DNMT colorimetric drug discovery tests

DNMT colorimetric drug discovery tests

Supplier: ENZO LIFE SCIENCES

DNMT colorimetric drug discovery kit is a simple method to detect DNA methylation activity or inhibition.

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ROS Detection Cell-Based Assay Kit (DHE)

ROS Detection Cell-Based Assay Kit (DHE)

Supplier: Cayman Chemical

Measure superoxide and hydrogen peroxide levels in living cells.

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Maybridge Screening library

Maybridge Screening library

Supplier: THERMO SCIENTIFIC MAYBRIDGE

The Maybridge portfolio consists of high-quality hit-like, lead-like and drug-like compounds which can generate valuable data in screening programs. They are developed specifically to support the pharmaceutical drug discovery process.

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CV-Cathepsin K detection kit

Supplier: ENZO LIFE SCIENCES

Based on the dye cresyl violet and the cathepsin K LeuArg peptide recognition sequence, this kit allows the detection of cathepsin K activity in living cells by fluorescence microscopy.

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ALDetect™ Lipid Peroxidation Assay kit

Supplier: ENZO LIFE SCIENCES

The assay is designed to measure either MDA alone (in hydrochloric acid) or MDA in combination with 4-hydroxyalkenals (in methanesulfonic acid). It is based on the reaction of a chromogenic reagent, N-methyl-2-phenylindole with MDA and 4-hydroxyalkenals to yield a stable chromophore with maximal absorbance at 586 nm.

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SREBP-1 transcription factor assay kit

SREBP-1 transcription factor assay kit

Supplier: Cayman Chemical

A 96-well assay for measurement of SREBP-1.

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LEADING LIGHT® Wnt Reporter Assay Starter Kit

Supplier: ENZO LIFE SCIENCES

The LEADING LIGHT® Wnt Reporter Assay Starter Kit is a cell-based luciferase activity test suitable for 96 well plate format.

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Caspase-3 assay kits, NucView™ 488

Caspase-3 assay kits, NucView™ 488

Supplier: Biotium

NucView® 488 caspase-3 substrate is a novel cell membrane-permeable fluorogenic caspase substrate designed for detecting caspase-3 activity within live cells in real time.

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Bacterial viability and gram stain kit

Supplier: Biotium

The Bacterial Viability and Gram Stain Kit contains three components: CF™488A-WGA, DAPI, and EthD-III solutions for distinguishing between gram-negative and gram-positive, as well as live versus dead bacteria.

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dsDNA quantitation kits for the Qubit® fluorometer, AccuGreen™, high-sensitivity and broad-range

dsDNA quantitation kits for the Qubit® fluorometer, AccuGreen™, high-sensitivity and broad-range

Supplier: Biotium

AccuGreen™ kit is specific for dsDNA and can quantify DNA samples in the range of 0,1 to 10 ng/μl.

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Hydrogen peroxide chemiluminescent detection Kit

Supplier: ENZO LIFE SCIENCES

For the quantitative determination of hydrogen peroxide in culture supernatants and cell lysates from any species.

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ROS-ID™ superoxide detection kit

Supplier: ENZO LIFE SCIENCES

This kit is designed to directly monitor real time superoxide production in live cells using fluorescence microscopy and/or flow cytometry.

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CF® Dye Dextran 70000 MW, anionic and fixable

CF® Dye Dextran 70000 MW, anionic and fixable

Supplier: Biotium

CF® dye labeled dextrans could be used as a fluorescent fluid-phase markers to study cell permeability, endocytosis, or mechanisms of biomolecular delivery. The dextran is 70,000 MW, and contains a formaldehyde-fixable free-amine group.

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CF® Dye Dextran 10000 MW, anionic and fixable

CF® Dye Dextran 10000 MW, anionic and fixable

Supplier: Biotium

CF® dye labeled dextrans could be used as a fluorescent fluid-phase markers to study cell permeability, endocytosis, or mechanisms of biomolecular delivery. The dextran is 10000 MW, and contains a formaldehyde-fixable free-amine group.

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CF® Dye Dextran 250000 MW, anionic and fixable

CF® Dye Dextran 250000 MW, anionic and fixable

Supplier: Biotium

CF® dye labeled dextrans could be used as a fluorescent fluid-phase markers to study cell permeability, endocytosis, or mechanisms of biomolecular delivery. The dextran is 250,000 MW, and contains a formaldehyde-fixable free-amine group.

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