Stains

Supplier: Merck

DPX new is a synthetic non aqueous mounting medium for microscopy. In its formulation, the teratogenic plasticiizer dibutyl phthalate (DBP) has been avoided, which renders the composition safer for the environment and more user friendly than the conventionally used mounting agents such as DPX.

Supplier: SIGMA ALDRICH MICROSCOPY

Bouin's solution is an excellent fixative for preserving soft and delicate structures. It is prepared with saturated picric acid, formaldehyde and acetic acid.

Supplier: SIGMA ALDRICH MICROSCOPY

D.P.X mounting medium is a mixture of distyrene (polystyrene), a plasticizer (tricresyl phosphate), and xylene. It facilitates the protection of stains and can dry quickly.

Supplier: SIGMA ALDRICH MICROSCOPY

Fluoroshield™ with DAPI is an aqueous mounting medium for preserving fluorescence of tissue and cell smears.

Supplier: SIGMA ALDRICH MICROSCOPY

Differentiation solution comprises acidified ethanol or isopropanol that is used to decolourise hematoxylin stain. The duration of exposure to the solution determines the degree of decolourisation. Differentiation is a crucial step in hematoxylin-eosin staining to achieve the proper colour contrast necessary for visualisation, by selectively removing excess hematoxylin from the tissue section and leaving behind the desired staining intensity.

Supplier: SIGMA ALDRICH MICROSCOPY

DPX Mountant for histology has been used as a slide mountant to fix sections.

Supplier: SIGMA ALDRICH MICROSCOPY

This is a mounting medium (contains 45% acrylic resin and 55% xylenes) used in microscopy. It is an adhesive and specimen preservative that can be used manually and in automated coverslipping equipment. Special purpose adhesive and sealant for use with glass, non plastic and other non-reactive materials.

Supplier: SIGMA ALDRICH MICROSCOPY

Fluoromount™ Aqueous Mounting Medium for use with fluorescent dye-stained tissue.

Supplier: Merck

M-FIX is a fixative spray designed for the wet fixation of gynaecological and non-gynaecological preparations processed in Papanicolaou staining. It contains polyglycols in alcoholic solution and a rapid and secure fixation of specimens is possible.

Supplier: SIGMA ALDRICH MICROSCOPY

Epoxy embedding medium is generally used for biological tissues during study by electron microscopy. Usually, it consists of three components; Epon 812 resin, an anhydride hardener and an accelerator

Supplier: SIGMA ALDRICH MICROSCOPY

Hartman′s fixative, which is also known as Davidson′s Fixative, contains acetic acid, alcohol, and formalin. It is widely used for the preservation of eye, bone marrow, breast, testis, and other tissues for histologic examination

Supplier: SIGMA ALDRICH MICROSCOPY

Araldite® is an epoxy resin commonly used for embedding. It is basically free from polymerization damage and the plastic does not sublime in the microscope, hence providing continual support and preservation of fine detail.[1] It is composed of araldite M or CY 212, dodecenyl succinic anhydride, dibutyl phthalate and 2,4,6-tridimethylamine methyl phenol.

Supplier: SIGMA ALDRICH MICROSCOPY

Fluoroshield™ with PPD (p-phenylenediamine; 1,4-phenylenediamine) is an aqueous mounting medium for preserving fluorescence of tissue and cell smears.

Supplier: SIGMA ALDRICH MICROSCOPY

JB-4 is a water-soluble, GMA based, plastic resin kit intended for use in the preparation of embedded samples for high resolution light microscopy. It is a technique now widely used for research and clinical diagnosis. JB-4 yields semi-thin sections (0.5μ-2μ) with excellent morphological preservation.

Supplier: Merck

Water-free mounting media, such as Neo-Mount®, should be used for permanent mounting and long-term storage of samples. Slides processed in pathologyl laboratories need to be stored up to 10 years, whereby the staining results need to be stable and fading-out is not acceptable. Neo-Mount®, anhydrous mounting medium for microscopy is an extremely colour-stable mounting medium for microscopy, which is produced with solvents based on mixtures of aliphatic hydrocarbons. It contains an aromatic-free substitute for xylene, thus, Neo-Mount® needs to be combined with Neo-Clear® exclusively. Xylene must be avoided in the mounting step, as it will cause the slides to become cloudy and streaked. In addition, by placing the dehydrated slides on filter paper for approx. 1 minute prior to mounting, any excess Neo-Clear® should be circumvented, as air bubbles might arise under the cover slip. The same pre-condition should also be met when mounting specimens using cover slip machines; in this area, Neo-Clear® can be most efficiently eliminated by incubating the slides for 1 minute in an empty slide rack.

Supplier: Merck

Entellan® new is a new mounting medium for cover slippers and is specially suited for commercial automated mounting instruments that operate with cover slippers. Its viscosity ranges between 250 and 600 mPas, 1,00869 has a small viscosity range. This narrow viscosity range improves and accelerates the application, as neither the dropping rate nor the volume need to be reset after the first run with a new bottle of Entellan® new.