Protein linking and labeling systems provide a convenient means for attaching fluorescent labels or biotins to antibodies and larger proteins. These kits are ideal for use with multiple applications, including flow cytometry, fluorescent microscopy, immunohistochemistry, primary detection, ELISAs, immunocytochemistry, and FISH. These systems are available with a variety of dye colors, biotin, fluorescent agents, and all the components needed to perform multiple conjugations and purifications. Protein labeling follows a simple protocol of reaction, separation, and then use.
Gold 525 dUTP
Supplier: ENZO LIFE SCIENCES
Gold 525 [5(6)-Carboxyrhodamine 6G] dUTP can replace TTP in reactions in which it serves as a substrate for E. coli DNA polymerase (holoenzyme and Klenow fragment), T4 and Taq DNA polymerases, reverse transcriptase (from AMV and M-MuLV) and terminal transferase. Fluorescently labelled probes can be prepared with this fluorescent nucleotide by a variety of methods including nick translation, random prime labelling, cDNA labelling and 3’-end labelling. Probes generated by these methods are suitable for use for the identification of specific sequences by in situ hybridization procedures on fixed cells and tissues by direct fluorescence detection. Gold 525 dUTP can also be used for multicolor fluorescence labelling.
Expand 1 Items
Buccutite™ Rapid PerCP Antibody Labelling Kits (2 Labellings)
Supplier: AAT BIOQUEST
PerCP (Peridinin-chlorophyll-protein complex) is isolated from Dinophyceae sp.
Expand 2 Items
DY647 CEP (DyLight 647 phosphoramidite)
Supplier: AAT BIOQUEST
DY647 CEP (DyLight 647 phosphoramidite) is an excellent replacement to the commonly used Cy5 phosphoramidite.
Expand 1 Items
ReadiLink™ Rapid iFluor® 633 Antibody Labelling Kit 'Microscale Optimised for Labelling 50 µg Antibody Per Reaction' (2 Labellings)
Supplier: AAT BIOQUEST
iFluor® dyes are developed for labelling proteins, in particular, antibodies.
Expand 1 Items
ReadiLink™ Rapid iFluor® 594 Antibody Labelling Kit 'Microscale Optimised for Labelling 50 µg Antibody Per Reaction' (2 Labellings)
Supplier: AAT BIOQUEST
AAT Bioquest's iFluor® dyes are optimised for labelling proteins, in particular, antibodies.
Expand 1 Items
ReadiLink™ Rapid iFluor® 700 Antibody Labeling Kit "Microscale Optimized for Labeling 50 µg Antibody Per Reaction" (2 Labelings)
Supplier: AAT BIOQUEST
AAT Bioquest's iFluor® dyes are developed for labelling proteins, in particular, antibodies.
Expand 1 Items
ReadiLink™ Rapid Cy7 Antibody Labelling Kit 'Microscale Optimised for Labelling 50 µg Antibody Per Reaction' (2 Labellings)
Supplier: AAT BIOQUEST
Cy7 is one of the most popular fluorescent labelling dyes for preparing orange-red fluorescent bioconjugates.
Expand 1 Items
ReadiLink™ Rapid iFluor® 647 Antibody Labelling Kit
Supplier: AAT BIOQUEST
ReadiLink™ Rapid antibody labelling kits, designed for production scale, provide a convenient and efficient method for labelling large volumes of antibodies with our superior iFluor® dyes.
Expand 1 Items
ReadiLink™ Rapid iFluor® 555 Antibody Labelling Kit
Supplier: AAT BIOQUEST
ReadiLink™ Rapid antibody labelling kits, designed for production scale, provide a convenient and efficient method for labelling large volumes of antibodies with our superior iFluor® dyes.
Expand 1 Items
ReadiLink™ Rapid iFluor® 594 Antibody Labelling Kit
Supplier: AAT BIOQUEST
ReadiLink™ Rapid antibody labelling kits, designed for production scale, provide a convenient and efficient method for labelling large volumes of antibodies with our superior iFluor® dyes.
Expand 1 Items
HIS Lite™ Cy5 Tris NTA-Ni Complex
Supplier: AAT BIOQUEST
Cy5-Tris NTA compound is used as a sensitive fluorescent probe for detecting polyhistidine-labelled proteins in cells, solution and solid surfaces.
Expand 1 Items
Fluor phosphine reagents, DyLight™, Pierce™
Supplier: Thermo Fisher Scientific
DyLight™ Fluor phosphine reagents are phosphine-activated fluorescent dyes for specific labeling and detection of azide-tagged molecules, which enables use of fluorescence imaging in metabolic labeling strategies.
Expand 3 Items
ReadiLink™ xtra Rapid iFluor® 488 Antibody Labeling Kit *BSA-Compatible* (2 Labelings)
Supplier: AAT BIOQUEST
ReadiLink™ xtra rapid antibody labelling kits require essentially only 2 simple mixing steps without a column purification needed.
Expand 1 Items
ReadiLink™ xtra Rapid iFluor® 594 Antibody Labeling Kit *BSA-Compatible* (2 Labelings)
Supplier: AAT BIOQUEST
ReadiLink™ xtra rapid antibody labelling kits require essentially only 2 simple mixing steps without a column purification needed.
Expand 1 Items
ReadiLink™ Rapid XFD750 Antibody Labelling Kit 'XFD750 Same Structure to Alexa Fluor™ 750' (2×50 µg Labellings)
Supplier: AAT BIOQUEST
XFD750 is manufactured by AAT Bioquest, and it has the same chemical structure of Alexa Fluor® 750 (Alexa Fluor® is the trademark of ThermoFisher).
Expand 1 Items
HIS Lite™ Cy3 Tris NTA Chelator
Supplier: AAT BIOQUEST
Cy3-Tris NTA compound is a sensitive fluorescent chelator for detecting polyhistidine-labelled proteins in cells, solution, and solid surfaces with the addition of certain metal ions (such as Ni²⁺, Co₂⁺ and more) as a mediator.
Expand 1 Items
HIS Lite™ iFluor® 647 Tris NTA Chelator
Supplier: AAT BIOQUEST
iFluor® 647 Tris-NTA Chelator is used as a sensitive red fluorescent probe for detecting polyhistidine-labelled proteins in combination with the addition of a heavy metal ion such as Ni²⁺ in cells, solution and solid surfaces.
Expand 1 Items
Carbohydrate reactive biotinylation reagents, EZ-Link™
Supplier: Thermo Fisher Scientific
A common target for protein modification is the carbohydrate portion of glycoproteins, which can be reacted with hydrazide derivatives of biotin. Oxidative treatment of glycoproteins using 10 mM periodate is used to generate reactive aldehydes from the cis-diols of a variety of carbohydrate moieties.
Expand 3 Items
Buccutite™ Rapid APC-iFluor® 750 Tandem Antibody Labelling Kit
Supplier: AAT BIOQUEST
Microscale optimised for labelling 25 µg antibody per reaction. APC-iFluor® 750 tandem is an excellent replacement for APC-Cy7 and APC-Alexa Fluor® 750 tandem since they have almost identical spectra.
Expand 1 Items
Avidin, Pierce™
Supplier: Thermo Fisher Scientific
Pierce™ Avidin is used in biotin-based detection methods, including Western blotting or ELISA detection with appropriate enzyme substrates. It is a purified protein from egg whites and it is stable over wide range of pH and temperature.
Expand 1 Items
Dye Labeling Kit (5/6) TAMRA-SE (Rhodamine), HOOK™
Supplier: G-Biosciences
The HOOK™ Dye Labeling Kit (TAMRA-SE (5-(and-6)- Carboxytetramethylrhodamine succinimidyl ester, mixed isomers) is based on tetramethylrhodamine, one of the most common fluorophores used in the labelling of peptides, proteins, nucleic acids and nucleotides. It absorbs green visible light at 546 nm and emits an orange-red visible light at a maximum emission of 575 nm.
Expand 2 Items
ReadiLink™ iFluor® 555 Nick Translation dsDNA Labelling Kit
Supplier: AAT BIOQUEST
It is compatible with a wide variety of sample materials, including bacterial artificial chromosome (BAC) DNA, human genomic DNA, purified PCR products, supercoiled and linearised plasmid DNA.
Expand 2 Items
Photoreactive biotin reagents, HOOK™ Psoralen-PEO-Biotin
Supplier: G-Biosciences
Photoreactive agents, on exposure to ultraviolet light, become active and bind non specifically with neighbouring molecules. These reagents are suitable for labelling molecules that do not contain easily reactable functional groups. Photoreactive reagent HOOK™ Psoralen-PEO-Biotin reacts with and labels nucleic acids and protein molecules. When reacted with nucleic acids, it crosslinks with pyrimidine bases. Crosslinking does not interfere with hybridisation applications.
Expand 1 Items
iFluor® 350-Wheat Germ Agglutinin (WGA) Conjugate
Supplier: AAT BIOQUEST
iFluor® 350 WGA conjugate, like Alexa Fluor® 350 WGA conjugate, retains its ability to bind to sialic acid and N-acetylglucosaminyl residues, enhancing its utility in fluorescence imaging and analysis for various scientific investigations.
Expand 1 Items
PSA™ iFluor® 350 Imaging Kit with Goat Anti-Rabbit IgG
Supplier: AAT BIOQUEST
iFluor® 350 PSA kit is a much superior replacement for Alexa Fluor 350 tyramide-based kit or other spectrally similar fluorescent tyramide or TSA kits.
Expand 1 Items
PSA™ iFluor® 350 Imaging Kit with Goat Anti-Mouse IgG
Supplier: AAT BIOQUEST
iFluor® 350 PSA kit is a much superior replacement for Alexa Fluor 350 tyramide-based kit or other spectrally similar fluorescent tyramide or TSA kits.
Expand 1 Items
PSA™ iFluor® 488 Imaging Kit with Goat Anti-Mouse IgG
Supplier: AAT BIOQUEST
iFluor® 488 PSA kit is a much superior replacement for Alexa Fluor 488 tyramide-based kit or other spectrally similar fluorescent tyramide or TSA kits.
Expand 1 Items
Carboxyl reactive biotin reagents, HOOK™
Supplier: G-Biosciences
HOOK™ Biotin-PEG₂-Amine, and its long chain form, HOOK™ Biotin-PEG₃-Amine, are carboxyl reactive biotinylation reagents. These agents contain terminal amines and react with carboxyl groups found at the carboxyl termini, aspartate, and glutamate side chains. The reaction is mediated by a water-soluble carbodiimide (EDC). The carbodiimide activates the carboxyl group and reacts with the amines (-NH₂) on the biotinylation agent to form an amide bond. This reaction is rapid and takes just a few minutes to complete. Under these conditions, hydrazide-derivatives of biotin reagents may also react with the carboxyls.
Expand 2 Items
Buccutite™ Rapid PE-Cy5 Tandem Antibody Labelling Kits (2 Labellings)
Supplier: AAT BIOQUEST
PE-Cy5 is a popular colour used in flow cytometry.
Expand 2 Items
Buccutite™ Rapid APC Antibody Labelling Kits (2 Labellings)
Supplier: AAT BIOQUEST
APC is an red fluorescent protein which has an excitation wavelength of 651 nm and an emission wavelength of 662 nm.