You searched for: Protein Linking and Labeling Systems

Supplier: G-Biosciences

Carboxyl coupling resin consists of 6% cross-linked agarose with covalently linked diaminodipropylamine (DADPA) to generate a free primary amine at the end of a long spacer arm. Molecules, including proteins, peptides and nucleic acids, are covalently coupled to the free primary amines, and the stable columns are ideal for affinity purification of antibodies and other interacting partners.

Supplier: Biotium

Kits for increasing immunofluorescence sensitivity using HRP-catalysed tyramide signal amplification with the choice of next-generation fluorescent CF® dyes or biotin.

Supplier: ENZO LIFE SCIENCES

Bio-N⁶-ddATP (Biotin-N⁶-(2’,3’-dideoxy)adenosine-5’ triphosphate) can be used for 3’-end labelling of single-stranded or double-stranded DNA. It is especially useful as a substrate for terminal transferase for the 3’-terminal addition of a single Bio-N⁶-ddAMP. It is also a substrate for Taq DNA Polymerase and other DNA polymerases.

Supplier: ENZO LIFE SCIENCES

Bio-7-dATP (Biotin-7-2’-deoxyadenosine-5’-triphosphate) can replace dATP in reactions in which it serves as a substrate for E. coli DNA polymerase (holoenzyme and Klenow fragment), T4 and Taq DNA polymerases, reverse transcriptase (from AMV and M-MuLV) and terminal transferase.

Supplier: ENZO LIFE SCIENCES

Allylamine-2’-deoxyuridine-5’-triphosphate (Allylamine-dUTP) can replace TTP in reactions where it serves as a substrate for E.coli DNA polymerase 1 (holoenzyme and Klenow fragment), terminal deoxynucloetide transferase, T4 and Taq DNA polymerases, and reverse transcriptases (from ALV and MLV).

Supplier: ENZO LIFE SCIENCES

Fluorescein-12-UTP is ideal for in vitro transcription reactions catalysed by T3, T7, or SP6 RNA polymerases and reverse transcriptase to produce fluorophore-labeled RNA transcripts. RNA-based probes can be used to identify complementary sequences by in situ hybridisation to fixed cells and tissues by direct or indirect fluorescence detection or by membrane hybridisation procedures.

Supplier: ENZO LIFE SCIENCES

Red 650 [Cyanine-5E] dUTP is a cyanine-5 derivative with enhanced spectral properties. This nucleotide analog can replace TTP in reactions in which it serves as a substrate for E.coli DNA polymerase (holoenzyme and Klenow fragment), T4 and Taq DNA polymerases, reverse transcriptase (from AMV and M-MuLV) and terminal transferase. Fluorescently labeled probes can be prepared with this fluorescent nucleotide by a variety of methods including nick translation, cDNA labelling and 3'-end labelling.

Supplier: ENZO LIFE SCIENCES

This kit offers a set of three different monoclonal antibodies specific for threonine phosphorylation sites. Phosphorylation patterns in a given cell extract may differ when probed with different antibodies due to sequence specificity. Recognition therefore depends on two criteria: Phosphorylation and surrounding aa motif. If one of the two criteria is not fulfilled, the antibody will not detect the phosphorylation site.

Supplier: Biotium

Phalloidin is a toxin isolated from the deadly Amanita phalloides mushroom. It is a bicyclic peptide that binds specifically to F-actin. Fluorescent conjugates of phalloidin are convenient tools to investigate the distribution of F-actin in fixed and permeabilised cells or tissue sections. One unit (U) of phallodin is sufficient to stain one sample (200 µl staining volume). A highly selective actin filament stain for fixed and permeabilised cells. Labeled phalloidins have similar affinity for both large and small filaments.

Supplier: ENZO LIFE SCIENCES

This kit offers a set of seven different MAbs specific for tyrosine phosphorylation sites. Phosphorylation patterns in a given cell extract may differ when probed with different antibodies due to sequence specificity. Recognition therefore depends on two criteria: Phosphorylation and surrounding aa motif. If one of the two criteria is not fulfilled, the antibody will not detect the phosphorylation site. Specificity has been determined by epitope mapping using degenerated phosphopeptide libraries.

Supplier: ENZO LIFE SCIENCES

Orange 552 dUTP (lyophilised)

Supplier: G-Biosciences

CDI amine reactive agarose consists of 6% cross-linked agarose activated with CDI (1,1’-carbonyl diimidazole) to form reactive imidazole carbamates.

Supplier: Biotium

A lectin labelled with our superior fluorescent CF® dyes is an effective and widely used cell surface stain for yeast, fungi, and mammalian cells.

Supplier: Biotium

Near-IR Annexin V fluorescent conjugates can be used to detect apoptotic cells and is suitable for in vivo imaging.

Supplier: Biotium

Fluorescent CF® dye tyramides are used for tyramide signal amplification (TSA) for increasing immunofluorescence sensitivity in multicolour immunocytochemistry (ICC), immunohistochemistry (IHC), or in situ hybridization (ISH).

Supplier: Biotium

Biotin picolyl azide reacts with alkyne to form 1,2,3-triazole by 1,3-dipolar Huisgen cycloaddition through the use of a much lower copper (I) concentration without sacrificing reaction efficiency.

Supplier: ENZO LIFE SCIENCES

TheORGANELLE-ID-RGB® III assay kit contains Golgi apparatus-selective, endoplasmic reticulum-selective and nucleus-selective dyes suitable for live cell staining.

Supplier: G-Biosciences

HOOK™ IgG biotinylation kits are designed for the biotinylation of IgG molecules by immobilising the IgG molecules on a solid support. Nickel chelating resin allows for the rapid removal of uncoupled biotin and therefore eliminates the need for further dialysis or desalting of the biotinylated antibody.

Supplier: Biotium

Carbocyanine dyes with long hydrocarbon chains have low fluorescence in aqueous phase, but become highly fluorescent upon binding to lipid bilayers, making this class of dyes ideal for staining cell membranes. Carbocyanine dye membrane staining is very stable, with minimal dye transfer between cells. Dilinoleyl carbocynaine dyes have much faster lateral diffusion rates than regular carbocyanine dyes, which facilitates labelling of neurons in tissues.

Supplier: ENZO LIFE SCIENCES

Orange 552 dUTP (5-TAMRA-dUTP) can replace TTP in reactions in which it serves as a substrate for E. coli DNA polymerase (holoenzyme and Klenow fragment), T4 and Taq DNA polymerases, reverse transcriptase (from AMV and M-MuLV) and terminal transferase. Fluorescently labeled probes can be prepared with this fluorescent nucleotide by a variety of methods including nick translation, cDNA labeling and 3’-end labeling. Probes generated by these methods are suitable for use for the identification of specific sequences by in situ hybridization procedures on fixed cells and tissues by direct fluorescence detection. Orange 552 dUTP can also be used for multicolor fluorescence labeling.

Supplier: ENZO LIFE SCIENCES

Red 580 dUTP (lyophilised)

Supplier: Biotium

Luciferin is a substrate for the enzyme firefly luciferase.

Supplier: Biotium

The simplicity and convenience of Mix-n-Stain™ labelling of nanobodies with fluorescent CF® dyes or biotin.

Supplier: ENZO LIFE SCIENCES

Improved data quality and analysis results through greater biotin incorporation.

Supplier: Biotium

INA (5-iodonaphthyl-1-azide) is a lipophilic photoreactive probe, which has been used to selectively label membrane-embedded cysteine residues of proteins.

Supplier: Rockland Immunochemicals

Peroxidase anti-peroxidase soluble complexes are used for peroxidase enzymatic labelling experiments.

Supplier: AGILENT

Luminescent peel-off stickers are used for accurately marking and aligning autoradiographs.

Supplier: Biotium

Reactive dyes containing an amine functional group.

Supplier: ENZO LIFE SCIENCES

The BIOARRAY™ Low Input RNA Amplification with Biotin Labeling System provides an optimised protocol and reagents for the production of biotin-labeled antisense RNA (aRNA) from total cellular RNA samples in less than 24 hours for array analysis.

Supplier: Apollo Scientific

Liquid product colourless to slightly yellow or greenish odourless pH 7,3 to 7,5 boiling point 104 °C. Stabiliser for controls and protein standards.