You searched for: Protein Linking and Labeling Systems

Protein linking and labeling systems provide a convenient means for attaching fluorescent labels or biotins to antibodies and larger proteins. These kits are ideal for use with multiple applications, including flow cytometry, fluorescent microscopy, immunohistochemistry, primary detection, ELISAs, immunocytochemistry, and FISH. These systems are available with a variety of dye colors, biotin, fluorescent agents, and all the components needed to perform multiple conjugations and purifications. Protein labeling follows a simple protocol of reaction, separation, and then use.

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Fluorescent dyes, M series, streptavidin conjugate

Supplier: Abnova

Fluorescent dye, M series, products are designed to be maximally excited by one of the major light sources equipped in flow cytometers. In comparison with phycobiliprotein tandems, Fluorescent dye, M series, possesses better photostability, higher conjugation yields, and little pH sensitivity making it an ideal choice in fluorescence imaging applications.

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CyDye Antibody Labeling Kits

Supplier: Cytiva

CyDye Fluorescent Dyes are supplied as highly water soluble, reactive NHS esters for easy conjugation to biomolecules containing aliphatic amino groups.

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Amersham™ CyDye™ Fluorescent Nucleotides

Supplier: Cytiva

Fluorescent nucleotides for labelling DNA and RNA probes, especially during multi-colour analysis in microarrays, FISH, chromosome identification, whole chromosome painting, karyotyping, and gene mapping.

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Monofunctional maleimide fluors, CyDye™

Supplier: Cytiva

Cy3 and Cy5 monofunctional maleimides are particularly suitable for the selective labelling of molecules containing free sulfhydryl groups, such as cysteine residues in proteins and peptides and oligonucleotides.

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Amersham CyDye DIGE fluors

Supplier: Cytiva

CyDye™ DIGE fluors are available as minimal and saturation labeling dyes. The minimal dyes are intended for general 2-D application use where sufficient amounts of sample are available. The saturation dyes from the Scarce Sample Labeling Kit are designed to be used for applications where only small amounts of sample are available, for example in Laser Capture Microdissection.

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Labeling dye, Atto 488, Sigma-Aldrich®

Supplier: Merck

Atto 488 is a labeling dye with high molecular absorption (90000) and quantum yield (0,80) as well as sufficient stokes shift between excitation and emission maximum. It is optimised for excitation with an argon laser, and is characterised by high photostability.

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DNA labeling reagent, Atto 647 NHS Ester

Supplier: Merck

Atto 647 NHS ester is a reagent for the covalent labeling of molecules with Atto 647 flourescent dye.

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Atto 647N-biotin, BioReagent

Supplier: Merck

Designed for high sensitivity applications, including single molecule detection. These labels have rigid structures that do not show any cis-trans-isomerisation and display exceptional intensity with minimal spectral shift on conjugation.

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Immunohistochemistry reagents, Biotinylated Anti-Streptavidin

Supplier: Vector Laboratories

Anti-streptavidin is produced by hyperimmunising goats with pure streptavidin and selecting out high affinity, specific antibody by affinity chromatography on specially prepared agarose bound streptavidin. Anti-streptavidin and anti-streptavidin conjugates can be used to localise streptavidin or to amplify the signal produced by streptavidin conjugates.

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Immunoperoxidase systems, VECTASTAIN® Elite® ABC Kit (Standard)

Supplier: Vector Laboratories

The VECTASTAIN® Elite® ABC kit is supplied without biotinylated secondary antibodies or blocking serum. The 'Standard' kit consists of Elite® A and B reagents, which can be used with any biotinylated primary or secondary antibody, biotinylated nucleic acid probe or other biotinylated molecule.

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Immunohistochemistry reagents, Horseradish Peroxidase Avidin D

Supplier: Vector Laboratories

Horseradish peroxidase avidin D is produced by our own coupling procedure which preserves the high specific activity of the peroxidase. Using biotinylated primary or secondary intermediates and peroxidase-labelled avidin D, antigens can be localised in histological sections, cytospin preparations, or smears. In addition, sensitive detection systems for a variety of solid-phase assays utilising microtitration plates or nitrocellulose have been developed using this conjugate.

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Peroxidase substrates, TrueBlue™

Supplier: SERACARE LIFE SCIENCES

KPL TrueBlue™ peroxidase substrate is a highly sensitive chromagenic substrate for visualisation of horseradish peroxidase-labeled reporter reagents.