Protein Extraction

Supplier: Abcam

Plasma membrane protein extraction kit provides optimized buffers and reagents for the effective extraction of plasma membrane proteins from mammalian tissues and cells. The kit offers the option to either extract total cellular membrane proteins or specifically purify plasma membrane proteins. - Cited in over 200 publications - High yield and purity – consistent yield with high purity (over 90%) - Suitable for western blotting, 2-D gels, enzyme analyses, and more

Supplier: G-Biosciences

FOCUS™ Global Fractionation kit is designed to fractionate complex biological samples into cytosolic and membrane fractions. The resulting membrane fractions are subsequently fractionated into either peripheral and integral membrane proteins or lipid raft associated proteins and detergent soluble membrane proteins.

Supplier: G-Biosciences

Extracts and solubilises nearly all of the proteins from mammalian samples, including membrane as well as soluble proteins, by a strong chaotropic extraction buffer to solubilise even the most difficult proteins. Suitable for biological samples from animal tissues and adherent and suspension cells.

Supplier: Thermo Fisher Scientific

The Pierce™ Mouse Embryonic Fibroblast (MEF) Isolation Kit enables high yield isolation and culture of viable primary fibroblasts from mouse embryos.

Supplier: Thermo Fisher Scientific

Pierce™ TMTzero™ Label Reagent serves as a "light" tag for MS-level peptide quantitation similar to duplex isotopic metabolic labeling, such as SILAC, or isotopic dimethylation labeling.

Supplier: Abcam

60-minute chromatin extraction kit is a complete set of optimized buffers and reagents for isolating chromatin or DNA-protein complex from mammalian cells and tissues in a simple and rapid format. - Cited in over 50 publications

Supplier: Abcam

This product is manufactured by BioVision, an Abcam company and was previously called 8002 EZLys™ Tissue Protein Extraction Reagent.

Supplier: Enzo Life Sciences

Rapid, reliable method to isolate nuclear proteins in mammalian cells and tissues.

Supplier: G-Biosciences

A reagent kit for detection and estimation of SDS in a sample. Mix the test sample in the extraction buffer reagents provided with the kit. If SDS is present in the sample, a blue colour is extracted that can be quantitatively measured.

Supplier: G-Biosciences

The F(ab)₂ Fragmentation kits are designed for the generation and isolation of F(ab)₂ fragments from IgG molecules. The kit utilises Immobilised Pepsin resin. Pepsin is a proteolytic enzyme that is routinely used for the generation of F(ab)₂ fragments from immunoglobulin G (IgG). One or more of the disulfide bonds that join the heavy chains in the hinge region are preserved, so the two Fab regions of the antibody remain joined together, yielding a divalent molecule (containing two antibody binding sites), hence the designation F(ab)₂. The light chains remain intact and attached to the heavy chain, whereas the Fc fragment is digested into small peptides. The Immobilised Pepsin offers the distinct advantage of eliminating enzyme contamination of the F(ab)₂ fragments.

Supplier: G-Biosciences

FOCUS™ PhosphoRich™ is a ready-to-use kit that enriches phosphorylated proteins and phosphopeptides from complex biological samples. The kit contains spin columns that have a phosphoprotein binding resin with a binding capacity of ~20 mg phosphorylated ovalbumin per column. The resin columns supplied with the kit can be reused, if regenerated and stored properly. FOCUS™ PhosphoRich™ is suitable for 2D gel electrophoresis and for proteomics and cell signalling studies.

Supplier: G-Biosciences

Antibody Fab Fragmentation Kits are designed for the generation and isolation of Fab fragments from IgG molecules. The kits utilise Immobilised Papain resin. Immobilised Papain offers the advantage of generating Fab and fragments without the need to remove the papain enzyme after digestion. Following papain digestion the Fab fragments are separated from undigested IgG and the Fc region with the supplied Protein A Spin Column. Protein A Resin binds the IgG and Fc molecules and the Fab are rapidly collected.

Supplier: G-Biosciences

OrgoSol DetergentOUT™ is suitable for removal of detergents from protein solutions, including hydrophobic protein solutions. Compatible with all detergent types, its performance is not dependent on the concentration of detergents in the solution. It is highly flexible and can be used to process small and large sample volumes.

Supplier: Abcam

ab110171 contains sufficient reagents for up to 20 benchtop mitochondria isolations from mammalian cultured cells.

Supplier: Thermo Fisher Scientific

Endoproteinase Lys-C protease is a highly purified native endoproteinase validated for maximum activity and stability in proteomic applications.

Supplier: Thermo Fisher Scientific

B-PER™ Complete Bacterial Protein Extraction Reagent is an all-in-one formulation of non ionic detergent and enzymes that enables mild extraction of proteins from both gram-negative and gram-positive bacteria without the need for mechanical disruption. B-PER Complete Reagent combines a universal nuclease and lysozyme to improve cell lysis and reduce viscosity, and may be used for soluble protein extraction and inclusion body purification from bacterial cell lysates.