Buffers

Resisting pH changes, buffers protect samples when acids or alkali is added. Powder and liquid forms efficiently track proteins during electrophoresis isolation and separation. The salty buffers can also be used to calibrate and standardize pH meters for accurate readings. Blocking substances stop antibody attachment to membrane free sites that would produce an interfering background. In various concentrations to fit any need, the buffers may come in liquids or dehydrated formats.

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Carbonate Borax Free Buffer Solution

Supplier: REAGECON

This range of Reagecon pH Buffers are sodium tetraborate (Borax) free. They are not subject to classification by EU regulatory authorities as toxic to reproduction under CLP category 1B. Similar in approach to the existing range of Reagecon Mercury Free Buffers, these Buffer Solutions have been developed to enhance user choice and allay safety concerns, without any impact on fitness for purpose or product performance.

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VWR® Buffered EDTA

Supplier: KLINIPATH

Buffered EDTA for decalcifying fluids.

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Phosphate Buffered Saline with 0,05% TWEEN® 20, pH 7,4

Supplier: Merck

Phosphate buffered saline with 0,05% Tween® 20 or PBST contains PBS buffer with a low-concentration of Tween® detergent. It is a common wash buffer and due to the presence of detergent, it can easily remove or block excess components thus reducing foaming and non-specific binding on the membrane and microtiter plate wells.

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Buffer, sodium acetate buffer solution pH 7 (3 mol/L), Sigma-Aldrich®

Supplier: Merck

Cloning Buffer, Sodium acetate buffer solution pH 7 (3 mol/l)

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Buffer, sodium acetate buffer solution pH 5,2 (3 mol/L), Sigma-Aldrich®

Supplier: Merck

Cloning Buffer, Sodium acetate buffer solution pH 5,2 (3 mol/l)

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Buffer, HEPES sodium salt solution (1 mol/L), BioReagent, suitable for cell culture, Sigma-Aldrich®

Supplier: Merck

HEPES sodium salt buffer solution (1 mol/l)

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pH Buffer solutions

Supplier: Hach

For calibration of pH meters.

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Buffer, TBE solution 10X concentrate (TRIS-borate-EDTA buffer), Sigma-Aldrich®

Supplier: Merck

TBE (Tris/Borate/EDTA) Buffer is commonly used in nucleic acid electrophoresis. This solution is effective under slightly basic conditions, which keeps DNA deprotonated, water-soluble, and protected from degradation. This concentrate can be easily diluted to 1X or 0,5X before use (with molecular biology grade water).

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Buffer, TRIS-glycine solution 10X concentrate (TG buffer), Sigma-Aldrich®

Supplier: Merck

Electrophoresis, Transfer buffer, TRIS-glycine buffer solution 10X concentrate (TG buffer)

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Buffer, TRIS solution pH 9,0 (1 mol/L), Sigma-Aldrich®

Supplier: Merck

Cloning Buffer, TRIS buffer solution pH 9,0 (1 mol/l)

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Triethylammonium bicarbonate buffers, for ion-exchange chromatography, Sigma-Aldrich®

Supplier: Merck

Buffer for use in ion-exchange chromatography and electrophoresis.

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Buffer, HEPES solution (1 mol/L), Sigma-Aldrich®

Supplier: Merck

HEPES buffer solution (1 mol/l)

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Phosphate buffered saline (PBS), Sigma-Aldrich®

Supplier: Merck

Contents of one pouch, when dissolved in one liter of distilled or deionised water, will yield 0,01 M phosphate buffered saline (NaCl 0,138 M; KCl - 0,0027 M); TWEEN® 20 - 0,05%, pH 7,4, at 25 °C.