Buffers

Supplier: Stemcell Technologies

PBS buffer containing EDTA.

Supplier: Serva

Cloning Buffer, TRIS buffer solution pH 8,8 (1 mol/l)

Supplier: Serva

Cloning Buffer, TE buffer solution 100x concentrate (TRIS-EDTA buffer)

Supplier: Stemcell Technologies

Ammonium chloride solution is recommended for the lysis of red blood cells (RBCs) in preparations of human and mouse peripheral blood, spleen or bone marrow cells. It is buffered and optimised for gentle lysis of erythrocytes, with minimal effect on leukocytes. It does not contain a fixative agent, therefore leukocytes are viable following RBC lysis.

Supplier: Molekula

Running Buffer, TAE buffer solution 10X concentrate (TRIS-acetate-EDTA buffer)

Supplier: Ambion

Hybridisation Buffer, SSC buffer solution 20X concentrate (saline-sodium citrate)

Supplier: Stemcell Technologies

Ideal for the permeabilisation of fixed cells during labeling of cytokines and other cytoplasmic antigens. Intracellular Permeabilisation Buffer (10X) is uniquely formulated for optimal resolution and to reduce background and is suitable for use with fluorochrome-conjugated antibodies for flow cytometry.

Supplier: Stemcell Technologies

Dulbecco’s phosphate-buffered saline (D-PBS) can be used as a temporary diluting, washing, irrigating or transporting solution for cell or tissue culture. It provides a buffering system to maintain the physiological pH range and osmotic balance of culture media and provides cells with a source of water and essential inorganic ions.

Supplier: UPSTATE TECHNOLOGY

Cloning Buffer, TE buffer solution (TRIS-EDTA buffer)

Supplier: Molekula

Cloning Buffer, TE buffer solution (TRIS-EDTA buffer) pH 7,5

Supplier: Cytiva

Phosphate buffered saline solution, available with or without detergent, recommended as running buffer for small molecule analysis in Biacore systems. Concentrated stock solution, yielding pH 7,4 when diluted 10X.