Buffers

Resisting pH changes, buffers protect samples when acids or alkali is added. Powder and liquid forms efficiently track proteins during electrophoresis isolation and separation. The salty buffers can also be used to calibrate and standardize pH meters for accurate readings. Blocking substances stop antibody attachment to membrane free sites that would produce an interfering background. In various concentrations to fit any need, the buffers may come in liquids or dehydrated formats.

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Triethylammonium bicarbonate buffers, for ion-exchange chromatography, Sigma-Aldrich®

Supplier: Merck

Buffer for use in ion-exchange chromatography and electrophoresis.

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Buffer, imidazole buffer solution (1 mol/L) BioUltra, Sigma-Aldrich®

Supplier: Merck

Imidazole buffer solution (1 mol/l)

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Glycine buffer, Sigma-Aldrich®

Supplier: Merck

For use in the enzymatic determination of lactate using lactic dehydrogenase.

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Phosphate buffered saline (PBS), Sigma-Aldrich®

Supplier: Merck

Contents of one pouch, when dissolved in one liter of distilled or deionised water, will yield 0,01 M phosphate buffered saline (NaCl 0,138 M; KCl - 0,0027 M); TWEEN® 20 - 0,05%, pH 7,4, at 25 °C.

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Buffer, sodium acetate buffer solution pH 7 (3 mol/L), Sigma-Aldrich®

Supplier: Merck

Cloning Buffer, Sodium acetate buffer solution pH 7 (3 mol/l)

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Buffer, sodium acetate buffer solution pH 5,2 (3 mol/L), Sigma-Aldrich®

Supplier: Merck

Cloning Buffer, Sodium acetate buffer solution pH 5,2 (3 mol/l)

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Buffer, HEPES sodium salt solution (1 mol/L), BioReagent, suitable for cell culture, Sigma-Aldrich®

Supplier: Merck

HEPES sodium salt buffer solution (1 mol/l)

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pH Buffer solutions

Supplier: Hach

For calibration of pH meters.

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Buffer, TBE solution 10X concentrate (TRIS-borate-EDTA buffer), Sigma-Aldrich®

Supplier: Merck

TBE (Tris/Borate/EDTA) Buffer is commonly used in nucleic acid electrophoresis. This solution is effective under slightly basic conditions, which keeps DNA deprotonated, water-soluble, and protected from degradation. This concentrate can be easily diluted to 1X or 0,5X before use (with molecular biology grade water).

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Buffer, TRIS-glycine solution 10X concentrate (TG buffer), Sigma-Aldrich®

Supplier: Merck

Electrophoresis, Transfer buffer, TRIS-glycine buffer solution 10X concentrate (TG buffer)

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Buffer, TRIS solution pH 9,0 (1 mol/L), Sigma-Aldrich®

Supplier: Merck

Cloning Buffer, TRIS buffer solution pH 9,0 (1 mol/l)

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Tris-EDTA buffer solution, pH 8,0, for molecular biology, BioUltra, Sigma-Aldrich®

Supplier: Merck

This buffer is 10 mM Tris-HCl and 1 mM disodium EDTA.

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Phosphate buffered saline (PBS), tablet, Sigma-Aldrich®

Supplier: Merck

Phosphate buffered saline (PBS) is a buffer solution used in biological research. It is a water based salt solution containing sodium phosphate, sodium chloride and, in some formulations, it contains potassium chloride and potassium phosphate.