Buffers
Resisting pH changes, buffers protect samples when acids or alkali is added. Powder and liquid forms efficiently track proteins during electrophoresis isolation and separation. The salty buffers can also be used to calibrate and standardize pH meters for accurate readings. Blocking substances stop antibody attachment to membrane free sites that would produce an interfering background. In various concentrations to fit any need, the buffers may come in liquids or dehydrated formats.
Succinate, 0,2 M buffer solution, pH 5,0
Supplier: Thermo Fisher Scientific
Succinate, 0.2M buffer soln., pH 5.0, Thermo Fisher Scientific
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Laemmli SDS sample buffer with pyronin Y, reducing (4X)
Supplier: Thermo Fisher Scientific
Loading Buffer, Laemmli SDS Sample Buffer, with PyroninY, 4X
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Buffer, HEPES solution pH 7,3 (1 mol/L), Molecular Biology Grade, Ultrapure
Supplier: Thermo Fisher Scientific
HEPES buffer solution pH 7.3 (1 mol/l)
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TES, 0,2 M buffer soln., pH 7,5
Supplier: Thermo Fisher Scientific
TES buffer solution, 0,2 M, pH value: 7,5, Liquid
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Glycine, 0.2M buffer soln., pH 2.5
Supplier: Thermo Fisher Scientific
Glycine buffer solution, 0,2 M, pH value: 2,5, Odourless, Liquid
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Buffer, sodium acetate buffer solution pH 5,2 (3 mol/L)
Supplier: Thermo Fisher Scientific
Cloning Buffer, Sodium acetate buffer solution pH 5,2 (3 mol/l)
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Buffer, sodium acetate buffer solution pH 5,2 (3 mol/L) AccuGENE®
Supplier: LONZA
Cloning Buffer, Sodium acetate buffer solution pH 5,2 (3 mol/l) AccuGENE®
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Buffer solutions, colour coded, Fluka™
Supplier: Honeywell Chemicals
These buffer solutions are colour-coded for easy identification and the pH 4 and 7 version have fungicide additive.
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Lysis buffers, red blood cells
Supplier: G-Biosciences
These DNase- and RNase-free buffers and reagents are designed for high quality research.
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Solubilisation buffer, 2D-Xtract™
Supplier: G-Biosciences
A protein solubilisation buffer for 2D analysis must solubilise proteins effectively, without disturbing the native charge of the proteins. Urea based solubilisation buffers solubilise proteins effectively, however can modify the native charge of the proteins, due to carbamylation. Urea exists in equilibrium with ammonium cyanate that modifies α- and ε-amino groups, inducing changes in the isoelectric point of proteins leading to artifactual results.
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IEF Anode buffer (50X)
Supplier: Thermo Fisher Scientific
Running Buffer, IEF Anode buffer (50X)
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PIPES lysis buffer with NP-40 (2X)
Supplier: Thermo Fisher Scientific
PIPES lysis buffer with NP-40 (2X), Thermo Fisher Scientific
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Phosphate-buffered saline (PBS, 5X), with EDTA
Supplier: Thermo Fisher Scientific
Phosphate-buffered saline (PBS) with EDTA, 5X, Liquid
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Glycine, 0.2M buffer soln., pH 3.0
Supplier: Thermo Fisher Scientific
Glycine buffer solution, 0,2 M, pH value: 3,0, Odourless, Liquid
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MES lysis buffer with Triton® X-100
Supplier: Thermo Fisher Scientific
MES lysis buffer with Triton® X-100, Thermo Fisher Scientific
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Loading buffer, SDS 4X, for SDS-PAGE analysis of proteins, Sigma-Aldrich®
Supplier: Merck Millipore (Novagen)
Loading Buffer, 4X SDS sample buffer
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#: 20864.320
Supplier: VWR Chemicals
#: 83640.320
Supplier: VWR Chemicals
#: 31770.294
Supplier: VWR Chemicals
#: 1.00030.2500
Supplier: Merck
#: 83645.320
Supplier: VWR Chemicals
#: 20060.320
Supplier: VWR Chemicals