Buffers

Resisting pH changes, buffers protect samples when acids or alkali is added. Powder and liquid forms efficiently track proteins during electrophoresis isolation and separation. The salty buffers can also be used to calibrate and standardize pH meters for accurate readings. Blocking substances stop antibody attachment to membrane free sites that would produce an interfering background. In various concentrations to fit any need, the buffers may come in liquids or dehydrated formats.

VWR® Separating Gel Buffer, 4X

Supplier: VWR Chemicals

4X concentrated buffer solution (1,5 M Tris, pH 8,8 and 0,4% SDS). Used for preparation of standard acrylamide:bis-acrylamide gels as well as preparation of gels using PAGE-PLUS™ acrylamide solution.

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VWR®, SSC buffer, 20X, ultra-pure grade

Supplier: VWR Chemicals

SSC buffer is a hybridisation buffer for Northern and Southern blots.

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VWR® A.C.E.™, Sequencing buffers

Supplier: VWR Chemicals

A.C.E.™ Sequencing Buffers are optimised buffers for DNA capillary electrophoresis sequencing that provide excellent results like long read lengths, high resolution, and consistent run times.

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VWR®, Eluent buffers for LC

Supplier: VWR Chemicals

LC eluent A, LUFA

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EDTA, 0.5 M solution, pH 8.0, for biotechnology

Supplier: VWR Chemicals

Cloning Buffer, EDTA, 0,5 M solution

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VWR®, TE (Tris-EDTA) buffer, pH 8,0 for biotechnology

Supplier: VWR Chemicals

Ready to use Tris-EDTA buffer.

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VWR® TBE Buffer Solution, 10X Concentrate (Tris-Borate-EDTA Buffer), UltraPure Grade

Supplier: VWR Chemicals

Running Buffer, TBE buffer solution 10X concentrate (Tris-borate-EDTA buffer)

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VWR® UltraPure, Buffer, PBS solution (phosphate buffered saline)

Supplier: VWR Chemicals

Dulbecco's formulation without calcium and magnesium.

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VWR®, Tris buffer solution, pH 9 (1 mol/L), ultrapure

Supplier: VWR Chemicals

Cloning Buffer, TRIS buffer solution pH 9 (1 mol/l)

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VWR® Phosphate Buffered Saline (PBS), Low Phosphate

Supplier: VWR Chemicals

Irradiated solution for use in washing cells.

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VWR® TISAB II Solution (Total Ionic Strength Adjustment Buffer) for Fluoride Determinations

Supplier: VWR Chemicals

Total ionic strength adjustment buffer solution for use with selective ion electrodes.

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VWR®, pH buffer capsules

Supplier: VWR Chemicals

pH buffer capsules, Capsules, pH value: 4,01, ±0,02, VWR®, Orange, Plastic bottle, 5 plastic tubes, 10 capsules/tube

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VWR® Acetate Buffer USP Test Solution (TS)

Supplier: VWR Chemicals

Ready to use test solutions (TS).

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VWR® Proteomics Grade, Tris-glycine buffer

Supplier: VWR Chemicals

A pre-blended Tris-glycine powder for preparation of Laemmli SDS-PAGE running buffer. The 40 litre pack size prepares 40 litres of 1X Tris-glycine solution (0,025 M Tris base and 0,192 M glycine). Each Ready-Pack™ prepares 1 litre of a 10X concentrate.

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VWR®, pH electrode maintenance kit

Supplier: VWR Chemicals

The measurement of the pH value is one of the most frequently and universally made measurements in science.

What is often not fully understood is the fact that a large number of problems related to pH measurement is the
correct selection, care or maintenance of the electrodes with particular emphasis on the reference electrode.