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You searched for: Buffers

Buffers

Resisting pH changes, buffers protect samples when acids or alkali is added. Powder and liquid forms efficiently track proteins during electrophoresis isolation and separation. The salty buffers can also be used to calibrate and standardize pH meters for accurate readings. Blocking substances stop antibody attachment to membrane free sites that would produce an interfering background. In various concentrations to fit any need, the buffers may come in liquids or dehydrated formats.

Phosphate buffered saline (PBS), tablet, Sigma-Aldrich®

Supplier: Merck

Phosphate buffered saline (PBS) is a buffer solution used in biological research. It is a water based salt solution containing sodium phosphate, sodium chloride and, in some formulations, it contains potassium chloride and potassium phosphate.

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Protein extraction reagent, BugBuster® HT, Millipore®

Supplier: Merck Millipore (Novagen)

BugBuster® HT Protein Extraction Reagent combines BugBuster® Protein Extraction Reagent and Benzonase® Nuclease in one convenient reagent. BugBuster® HT eliminates common bioprocessing problems resulting from traditional cell lysis procedures. Soluble proteins are gently extracted from E. coli without exposure to heat or oxidative damage and viscosity is eliminated by nucleic acid digestion in a single step. The resulting protein extract can easily be fractionated by conventional purification techniques. BugBuster HT is ideally suited for application in high throughput protein purifications.

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Phosphate buffered saline (PBS), Sigma-Aldrich®

Supplier: Merck

Contents of one pouch, when dissolved in one liter of distilled or deionised water, will yield 0,01 M phosphate buffered saline (NaCl 0,138 M; KCl - 0,0027 M); TWEEN® 20 - 0,05%, pH 7,4, at 25 °C.

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Phosphate buffered saline, 10X, for Western blots and IP, Sigma-aldrich®

Supplier: Merck

Phosphate buffered saline is routinely used as a wash buffer in Western blot and immunoprecipitation procedures, as well as during sample preparation of cell lysates for use in general molecular biology applications.

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Glycine buffer, Sigma-Aldrich®

Supplier: Merck

For use in the enzymatic determination of lactate using lactic dehydrogenase.

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Tris Hydrochloride

Supplier: Merck Millipore (Calbiochem‎)

Use of tetraethylammonium (TEA) and Tris loading for blocking TRPM7 channels in intact cells.: This study highlights the application of TRIS Hydrochloride in blocking TRPM7 channels, which is significant for cell signaling and ion channel regulation in pharmacological research (Holderby & Kozak, 2024).

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Phosphate Buffered Saline with 0,05% TWEEN® 20, pH 7,4

Supplier: Merck

Phosphate buffered saline with 0,05% Tween® 20 or PBST contains PBS buffer with a low-concentration of Tween® detergent. It is a common wash buffer and due to the presence of detergent, it can easily remove or block excess components thus reducing foaming and non-specific binding on the membrane and microtiter plate wells.

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pH buffer solutions

pH buffer solutions

Supplier: Mettler - Toledo

Technical buffer solution, 6×250 ml, pH value: 4,64, Neutral, 250 ml

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Buffer solutions

Buffer solutions

Supplier: REAGECON

Buffer solution, pH value: 4,00, ±0,01, Reference temperature: 20 °C, Plastic bottle

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Buffer capsules, coloured coded

Supplier: REAGECON

pH buffer capsules, reference standard, pH value: 10,00, ±0,02, Reference temperature: 25 °C, Blue

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Dulbecco’s phosphate buffered saline (DPBS), Sigma-Aldrich®

Supplier: Merck

Dulbecco’s phosphate buffered saline is modified with/without calcium chloride and magnesium chloride, liquid, sterile-filtered, suitable for cell culture.

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Hanks' Balanced Salts (Modified), 1X, without Magnesium and Calcium, MP Biomedicals

Hanks' Balanced Salts (Modified), 1X, without Magnesium and Calcium, MP Biomedicals

Supplier: MP Biomedicals

Used to minimise cell attachment or aggregation.

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pH electrode calibration and maintenance solutions kit

pH electrode calibration and maintenance solutions kit

Supplier: WTW

Adapted to different electrode types for calibration and maintenance.

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pH buffer solutions

pH buffer solutions

Supplier: Hach

pH buffer standard solution with certificate of analysis, 125 ml, pH value: 9,21, Plastic bottle

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Hank's balanced salts, MP Biomedicals

Supplier: MP Biomedicals

Hanks' balanced salts solution is used to maintain the pH and osmotic balance in the medium and to provide the cells with water and essential inorganic ions.

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pH buffer, single-use, SINGLET™

pH buffer, single-use, SINGLET™

Supplier: Hach

These buffers solutions packs are used for pH meter calibration. The sealed foil pouches can be transported easily and safely without any spillage and also prevents contamination or exposure to air of the solutions.

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pH buffer solutions, colour coded

Supplier: REAGECON

Colour coded pH buffer solutions for the calibration of pH meters.

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pH Buffer solutions

pH Buffer solutions

Supplier: Hach

For calibration of pH meters.

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Buffer, TBE solution 10X concentrate (TRIS-borate-EDTA buffer)

Supplier: Uptima

Running Buffer, TBE buffer solution 10X concentrate (TRIS-borate-EDTA buffer)

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Buffer, TAE solution 25X concentrate (TRIS-acetate-EDTA buffer)

Supplier: Uptima

Running Buffer, TAE buffer solution 25X concentrate (TRIS-acetate-EDTA buffer)

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Carbonate buffer concentrate

Supplier: YSI LIFE SCIENCE

Carbonate buffer concentrate for use with methanol membranes and ethanol membranes. Reconstitute to make 1 litre.

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Saline solutions

Supplier: BALIS

Cell culture buffer, Physiological saline solution, 9 ml, no standards

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Triethylammonium bicarbonate buffers, for ion-exchange chromatography, Sigma-Aldrich®

Supplier: Merck

Buffer for use in ion-exchange chromatography and electrophoresis.

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Buffer, SSC solution 20X concentrate (saline-sodium citrate)

Supplier: SERACARE LIFE SCIENCES

Hybridisation Buffer, SSC buffer solution 20X concentrate (saline-sodium citrate)

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Buffer, PBS, Powder pack for 10L 1X

Supplier: Uptima

Washing buffer, PBS, Powder pack for 10L 1X

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Tris-EDTA buffer solution, pH 8,0, for molecular biology, BioUltra, Sigma-Aldrich®

Supplier: Merck

This buffer is 10 mM Tris-HCl and 1 mM disodium EDTA.

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Buffer, SSC solution 20X concentrate (saline-sodium citrate)

Supplier: Uptima

Hybridisation Buffer, SSC buffer solution 20X concentrate (saline-sodium citrate)

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pH Standard Solutions, IUPAC, Certified

pH Standard Solutions, IUPAC, Certified

Supplier: Hach

Buffers traceable to IUPAC/NIST pH scale.

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Buffer tablets, Lovibond®

Supplier: Lovibond

Ideal for preparing buffer solutions of 4, 7 and 10 pH units.

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