You searched for: Buffers
Resisting pH changes, buffers protect samples when acids or alkali is added. Powder and liquid forms efficiently track proteins during electrophoresis isolation and separation. The salty buffers can also be used to calibrate and standardize pH meters for accurate readings. Blocking substances stop antibody attachment to membrane free sites that would produce an interfering background. In various concentrations to fit any need, the buffers may come in liquids or dehydrated formats.
Phosphate buffered saline (PBS), tablet, Sigma-Aldrich®
Supplier: Merck
Phosphate buffered saline (PBS) is a buffer solution used in biological research. It is a water based salt solution containing sodium phosphate, sodium chloride and, in some formulations, it contains potassium chloride and potassium phosphate.
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Protein extraction reagent, BugBuster® HT, Millipore®
Supplier: Merck Millipore (Novagen)
BugBuster® HT Protein Extraction Reagent combines BugBuster® Protein Extraction Reagent and Benzonase® Nuclease in one convenient reagent. BugBuster® HT eliminates common bioprocessing problems resulting from traditional cell lysis procedures. Soluble proteins are gently extracted from E. coli without exposure to heat or oxidative damage and viscosity is eliminated by nucleic acid digestion in a single step. The resulting protein extract can easily be fractionated by conventional purification techniques. BugBuster HT is ideally suited for application in high throughput protein purifications.
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Phosphate buffered saline (PBS), Sigma-Aldrich®
Supplier: Merck
Contents of one pouch, when dissolved in one liter of distilled or deionised water, will yield 0,01 M phosphate buffered saline (NaCl 0,138 M; KCl - 0,0027 M); TWEEN® 20 - 0,05%, pH 7,4, at 25 °C.
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Phosphate buffered saline, 10X, for Western blots and IP, Sigma-aldrich®
Supplier: Merck
Phosphate buffered saline is routinely used as a wash buffer in Western blot and immunoprecipitation procedures, as well as during sample preparation of cell lysates for use in general molecular biology applications.
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Glycine buffer, Sigma-Aldrich®
Supplier: Merck
For use in the enzymatic determination of lactate using lactic dehydrogenase.
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Buffer, HEPES sodium salt solution (1 mol/L), BioReagent, suitable for cell culture, Sigma-Aldrich®
Supplier: Merck
HEPES sodium salt buffer solution (1 mol/l)
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Tris Hydrochloride
Supplier: Merck Millipore (Calbiochem)
Use of tetraethylammonium (TEA) and Tris loading for blocking TRPM7 channels in intact cells.: This study highlights the application of TRIS Hydrochloride in blocking TRPM7 channels, which is significant for cell signaling and ion channel regulation in pharmacological research (Holderby & Kozak, 2024).
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Phosphate Buffered Saline with 0,05% TWEEN® 20, pH 7,4
Supplier: Merck
Phosphate buffered saline with 0,05% Tween® 20 or PBST contains PBS buffer with a low-concentration of Tween® detergent. It is a common wash buffer and due to the presence of detergent, it can easily remove or block excess components thus reducing foaming and non-specific binding on the membrane and microtiter plate wells.
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pH buffer solutions
Supplier: Mettler - Toledo
Technical buffer solution, 6×250 ml, pH value: 4,64, Neutral, 250 ml
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Buffer solutions
Supplier: REAGECON
Buffer solution, pH value: 4,00, ±0,01, Reference temperature: 20 °C, Plastic bottle
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Buffer capsules, coloured coded
Supplier: REAGECON
pH buffer capsules, reference standard, pH value: 10,00, ±0,02, Reference temperature: 25 °C, Blue
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Dulbecco’s phosphate buffered saline (DPBS), Sigma-Aldrich®
Supplier: Merck
Dulbecco’s phosphate buffered saline is modified with/without calcium chloride and magnesium chloride, liquid, sterile-filtered, suitable for cell culture.
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Hanks' Balanced Salts (Modified), 1X, without Magnesium and Calcium, MP Biomedicals
Supplier: MP Biomedicals
Used to minimise cell attachment or aggregation.
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pH electrode calibration and maintenance solutions kit
Supplier: WTW
Adapted to different electrode types for calibration and maintenance.
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pH buffer solutions
Supplier: Hach
pH buffer standard solution with certificate of analysis, 125 ml, pH value: 9,21, Plastic bottle
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Hank's balanced salts, MP Biomedicals
Supplier: MP Biomedicals
Hanks' balanced salts solution is used to maintain the pH and osmotic balance in the medium and to provide the cells with water and essential inorganic ions.
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pH buffer, single-use, SINGLET™
Supplier: Hach
These buffers solutions packs are used for pH meter calibration. The sealed foil pouches can be transported easily and safely without any spillage and also prevents contamination or exposure to air of the solutions.
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pH buffer solutions, colour coded
Supplier: REAGECON
Colour coded pH buffer solutions for the calibration of pH meters.
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Buffer, TBE solution 10X concentrate (TRIS-borate-EDTA buffer)
Supplier: Uptima
Running Buffer, TBE buffer solution 10X concentrate (TRIS-borate-EDTA buffer)
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Buffer, TAE solution 25X concentrate (TRIS-acetate-EDTA buffer)
Supplier: Uptima
Running Buffer, TAE buffer solution 25X concentrate (TRIS-acetate-EDTA buffer)
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Carbonate buffer concentrate
Supplier: YSI LIFE SCIENCE
Carbonate buffer concentrate for use with methanol membranes and ethanol membranes. Reconstitute to make 1 litre.
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Saline solutions
Supplier: BALIS
Cell culture buffer, Physiological saline solution, 9 ml, no standards
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Triethylammonium bicarbonate buffers, for ion-exchange chromatography, Sigma-Aldrich®
Supplier: Merck
Buffer for use in ion-exchange chromatography and electrophoresis.
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Buffer, SSC solution 20X concentrate (saline-sodium citrate)
Supplier: SERACARE LIFE SCIENCES
Hybridisation Buffer, SSC buffer solution 20X concentrate (saline-sodium citrate)
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Buffer, PBS, Powder pack for 10L 1X
Supplier: Uptima
Washing buffer, PBS, Powder pack for 10L 1X
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Tris-EDTA buffer solution, pH 8,0, for molecular biology, BioUltra, Sigma-Aldrich®
Supplier: Merck
This buffer is 10 mM Tris-HCl and 1 mM disodium EDTA.
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Buffer, SSC solution 20X concentrate (saline-sodium citrate)
Supplier: Uptima
Hybridisation Buffer, SSC buffer solution 20X concentrate (saline-sodium citrate)
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pH Standard Solutions, IUPAC, Certified
Supplier: Hach
Buffers traceable to IUPAC/NIST pH scale.
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Buffer tablets, Lovibond®
Supplier: Lovibond
Ideal for preparing buffer solutions of 4, 7 and 10 pH units.