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You searched for: Buffers

Buffers

Resisting pH changes, buffers protect samples when acids or alkali is added. Powder and liquid forms efficiently track proteins during electrophoresis isolation and separation. The salty buffers can also be used to calibrate and standardize pH meters for accurate readings. Blocking substances stop antibody attachment to membrane free sites that would produce an interfering background. In various concentrations to fit any need, the buffers may come in liquids or dehydrated formats.

ISE sample conditioning buffers and solutions

Supplier: WTW

Ion strength adjustment solutions have multiple tasks: TISAB for fluoride measurements does not only adjust the pH value, by also decomplexes the fluoride-containing complex compounds. Other ion strength adjustment solutions ensure an adapted pH value as well as the addition of a suitable conducting salt to correctly capture the concentration.

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Buffer, sodium acetate buffer solution pH 7 (3 mol/L), Sigma-Aldrich®

Supplier: Merck

Cloning Buffer, Sodium acetate buffer solution pH 7 (3 mol/l)

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Buffer, imidazole buffer solution (1 mol/L) BioUltra, Sigma-Aldrich®

Supplier: Merck

Imidazole buffer solution (1 mol/l)

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Buffer, TRIS solution pH 8,0 (2 mol/L), Sigma-Aldrich®

Supplier: Merck

Cloning Buffer, TRIS buffer solution pH 8,0 (2 mol/l)

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Buffer, TRIS solution pH 8,0 (1 mol/L), Sigma-Aldrich®

Supplier: Merck

Cloning Buffer, TRIS buffer solution pH 8,0 (1 mol/l)

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VWR® Buffered EDTA

Supplier: KLINIPATH

Buffered EDTA for decalcifying fluids.

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Buffer, sodium acetate buffer solution pH 5,2 (3 mol/L), Sigma-Aldrich®

Supplier: Merck

Cloning Buffer, Sodium acetate buffer solution pH 5,2 (3 mol/l)

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Buffer solutions, colour coded, certified (20 °C), Fluka™

Supplier: Honeywell Chemicals

Borate, pH value: 9.00, 8.992 - 9.008, Reference temperature: 20 °C, Yellow

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Buffer, TRIS solution pH 7,2 (1 mol/L), Sigma-Aldrich®

Supplier: Merck

Cloning Buffer, TRIS buffer solution pH 7,2 (1 mol/l)

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Buffer, TRIS solution pH 7,5 (1 mol/L), Sigma-Aldrich®

Supplier: Merck

Cloning Buffer, TRIS buffer solution pH 7,5 (1 mol/l)

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Protein extraction reagent, BugBuster® (primary amine-free), Millipore®

Supplier: Merck Millipore (Novagen)

BugBuster® (primary amine-free) Protein Extraction Reagent is a special formulation of BugBuster designed for applications where primary amines would interfere if present in the protein extract, such as protein immobilisation or cross-linking. The PIPPS buffer used in BugBuster® (primary amine-free) has a similar buffer capacity and pH range as the original Tris-buffered BugBuster®, but will not complex metal ions, also making it ideally suited for extraction of metal dependent proteins.

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Buffer, TRIS solution pH 7,6 (1 mol/L), Sigma-Aldrich®

Supplier: Merck

Cloning Buffer, TRIS buffer solution pH 7,6 (1 mol/l)

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Buffer, TRIS solution pH 8,0 (1 mol/L), Sigma-Aldrich®

Supplier: Merck

Cloning Buffer, TRIS buffer solution pH 8,0 (1 mol/l)

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Buffer, TRIS solution pH 7,4 (1 mol/L), Sigma-Aldrich®

Supplier: Merck

Cloning Buffer, TRIS buffer solution pH 7,4 (1 mol/l)

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Buffer, TRIS solution pH 7,8 (1 mol/L), Sigma-Aldrich®

Supplier: Merck

Cloning Buffer, TRIS buffer solution pH 7,8 (1 mol/l)

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Buffer, HEPES solution (1 mol/L), Sigma-Aldrich®

Supplier: Merck

HEPES buffer solution (1 mol/l)

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Protein extraction reagent, BugBuster® 10X, Millipore®

Supplier: Merck Millipore (Novagen)

BugBuster® 10X is a concentrated formulation of the proprietary detergents employed in BugBuster® without the addition of salts or buffer components. Concentrated BugBuster® provides a flexible alternative to the ready-to-use standard 1X BugBuster®, allowing user-defined dilution and addition of buffer components. BugBuster® 10X has all of the bioprocessing benefits of standard BugBuster® plus the freedom to control pH, reagent concentration, and buffer additives necessary for maximum extraction and activity of your target protein.

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Buffer, TRIS solution pH 9,0 (1 mol/L), Sigma-Aldrich®

Supplier: Merck

Cloning Buffer, TRIS buffer solution pH 9,0 (1 mol/l)

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Buffer, TRIS-tricine-SDS solution 10X concentrate, Sigma-Aldrich®

Supplier: Merck

Running Buffer, TRIS-tricine-SDS buffer solution 10X concentrate

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Buffer, SSC solution 20X concentrate (saline-sodium citrate), Sigma-Aldrich®

Supplier: Merck

Hybridisation Buffer, SSC buffer solution 20X concentrate (saline-sodium citrate)

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Buffer, TBE solution 10X concentrate (TRIS-borate-EDTA buffer), Sigma-Aldrich®

Supplier: Merck

TBE (Tris/Borate/EDTA) Buffer is commonly used in nucleic acid electrophoresis. This solution is effective under slightly basic conditions, which keeps DNA deprotonated, water-soluble, and protected from degradation. This concentrate can be easily diluted to 1X or 0,5X before use (with molecular biology grade water).

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Tris Buffered Saline, 10X concentrate solution, Sigma-Aldrich®

Supplier: Merck

Washing buffer for alkaline phosphatase or peroxidase conjugates in Western blotting.

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T-PER™ Tissue Protein Extraction Reagent

T-PER™ Tissue Protein Extraction Reagent

Supplier: Thermo Fisher Scientific

Tissue Protein Extraction Reagent (T-PER ) enables mild extraction of total protein from tissue samples.

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Protein extraction reagents, BugBuster® and BugBuster® plus Benzonase®, Millipore®

Supplier: Merck Millipore (Novagen)

BugBuster® Protein Extraction reagent is formulated for the gentle disruption of the cell wall of E. coli, liberating soluble protein. It provides a simple, rapid, low-cost alternative to mechanical methods such as French Press or sonication for releasing expressed target protein in preparation for purification or other applications. The formulation utilises a detergent mix that is capable of cell wall perforation without denaturing soluble protein.

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Buffer, SSC solution 20X concentrate (saline-sodium citrate), Sigma-Aldrich®

Supplier: Merck

Hybridisation Buffer, SSC buffer solution 20X concentrate (saline-sodium citrate)

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Buffer solutions

Buffer solutions

Supplier: SI Analytics

Buffer solutions intended for extremely precise calibration.

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Protein extraction reagent, BugBuster® HT, Millipore®

Supplier: Merck Millipore (Novagen)

BugBuster® HT Protein Extraction Reagent combines BugBuster® Protein Extraction Reagent and Benzonase® Nuclease in one convenient reagent. BugBuster® HT eliminates common bioprocessing problems resulting from traditional cell lysis procedures. Soluble proteins are gently extracted from E. coli without exposure to heat or oxidative damage and viscosity is eliminated by nucleic acid digestion in a single step. The resulting protein extract can easily be fractionated by conventional purification techniques. BugBuster HT is ideally suited for application in high throughput protein purifications.

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TISAB III solution (Total Ionic Strength Adjustment Buffer) for fluoride determinations

TISAB III solution (Total Ionic Strength Adjustment Buffer) for fluoride determinations

Supplier: Mettler - Toledo

TISAB III solution for fluoride determinations

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