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You searched for: Buffers

Buffers

Resisting pH changes, buffers protect samples when acids or alkali is added. Powder and liquid forms efficiently track proteins during electrophoresis isolation and separation. The salty buffers can also be used to calibrate and standardize pH meters for accurate readings. Blocking substances stop antibody attachment to membrane free sites that would produce an interfering background. In various concentrations to fit any need, the buffers may come in liquids or dehydrated formats.

Subcellular Protein Fractionation Kit for Cultured Cells

Subcellular Protein Fractionation Kit for Cultured Cells

Supplier: Thermo Fisher Scientific

This kit is ideal for segregation and enrichment of proteins from five different cellular compartments.

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HEPES protein extraction buffer (5X)

Supplier: Thermo Fisher Scientific

Thermo Fisher Scientific

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Buffer, sodium acetate, 3M aq, soln,, pH 4,5, autoclaved

Supplier: Thermo Fisher Scientific

Sodium acetate, 3M aq, solution, pH 4,5, autoclaved

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Buffer, sodium acetate, 3 M aq, soln,, pH 7,0, RNAse free

Supplier: Thermo Fisher Scientific

Sodium acetate, 3M aq, solution, pH 7,0, RNAse free, Liquid

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EDTA, 0.5M pH 8.0, Corning®

EDTA, 0.5M pH 8.0, Corning®

Supplier: Corning

EDTA (ethylenediaminetetraacetic acid) is a polyamino carboxylic acid. It is a colorless, water-soluble solid and is useful as a chelating agent due to its ability to "sequester" metal ions such as Ca2+ and Fe3+.  These cations are responsible for various undesirable effects in molecular biological and cell culture processes. After being bound by EDTA, these metal ions remain in the solution but exhibit diminished reactivity.  The use of EDTA therefore allows for greater control over many experiments.

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Buffer solutions as per DIN 19266-01: 2000

Buffer solutions as per DIN 19266-01: 2000

Supplier: WTW

These standard buffer solutions (work reference buffer solutions) are widely used in precision applications.

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Mini flip-top vial with 9 ml Letheen Broth

Mini flip-top vial with 9 ml Letheen Broth

Supplier: 3M Food Safety

The 3M™ Mini Flip-Top Vial with 9 ml Letheen Broth is a leak-resistant plastic cylinder with a flip-open lid that seals securely for safe shipping and handling.

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I-PER™ Insect Cell Protein Extraction Reagents

I-PER™ Insect Cell Protein Extraction Reagents

Supplier: Thermo Fisher Scientific

The I-PER Insect Cell Protein Extraction Reagent enables gentle and effective extraction of soluble protein from baculovirus-infected insect cells grown in suspension or monolayer culture. The baculovirus insect cell expression system is an efficient and popular system for production of recombinant (eukaryotic) proteins in cell culture. Proteins expressed in baculoviral systems can be used for structural analyses, biochemical assays and a variety of other applications. I-PER Reagent maintains functionality of extracted proteins and is directly compatible with downstream applications such as protein assays, Western blotting and 6xHis-tagged protein purification.

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Buffer solution pH 7, Phosphate buffer

Supplier: Thermo Fisher Scientific

Potassium dihydrogen phosphate and di-Sodium hydrogen phosphate in aqueous solution, Phosphate, pH value: 7,00, ±0,02

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Buffer, sodium acetate, 3 M aq, soln,, pH 7,0, autoclaved

Supplier: Thermo Fisher Scientific

Sodium acetate, 3M aq, solution, pH 7,0, autoclaved

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Buffer, PBS solution (phosphate buffered saline) pH 7,4, Sigma-Aldrich®

Supplier: Merck

Washing buffer, PBS buffer solution (phosphate buffered saline) pH 7,4

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Buffer, TRIS-glycine-SDS solution 10X concentrate (TGS buffer), Sigma-Aldrich®

Supplier: Merck

Running Buffer, TRIS-glycine-SDS buffer solution 10X concentrate (TGS buffer)

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10% SDS (Sodium Dodecyl Sulfate), Corning®

10% SDS (Sodium Dodecyl Sulfate), Corning®

Supplier: Corning

Sodium Dodecyl Sulfate (SDS) is an anionic detergent with applications in SDS-PAGE protein analysis and nucleic acid purification. It is a wetting agent that is effective in both acid and alkaline solutions, and is most often used as a protein and lipid solubilization reagent. SDS is also a powerful protein denaturant. This 10% solution is prepared in 18 megaohm water and is 0.2µm filtered.

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Ionisation buffer solutions (CRMs), for AAS and ICP, Fluka™

Supplier: Honeywell Chemicals

Matrix Modifier, 2% Potassium (K) in 1% HCl 1%

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Buffer, PBS solution 10X concentrate (phosphate buffered saline), Sigma-Aldrich®

Supplier: Merck

Washing buffer, PBS buffer solution 10X concentrate (phosphate buffered saline)

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Buffer, sodium acetate, 1M aq, soln,, pH 4,5, RNAse free

Supplier: Thermo Fisher Scientific

Sodium acetate, 1M aq, solution, pH 4,5, RNAse free, Odourless, Liquid

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Buffer, TRIS-glycine solution 10X concentrate (TG buffer), Sigma-Aldrich®

Supplier: Merck

Electrophoresis, Transfer buffer, TRIS-glycine buffer solution 10X concentrate (TG buffer)

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Phosphate Buffer Mixed pH 4,0

Supplier: CPACHEM

Shelf Life on Ship Date: 12 months - Store under normal laboratory conditions, at temperatures between 15 to 25 °C.

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pH buffers, ready to use

pH buffers, ready to use

Supplier: WTW

Every bottle has its own lot reference with the expiration date.

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Buffer, TE solution 100x concentrate (TRIS-EDTA buffer), Sigma-Aldrich®

Supplier: Merck

Cloning Buffer, TE buffer solution 100x concentrate (TRIS-EDTA buffer)

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Buffer, PBS solution 10X concentrate (phosphate buffered saline), Sigma-Aldrich®

Supplier: Merck

Washing buffer, PBS buffer solution 10X concentrate (phosphate buffered saline)

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Buffer, PBS solution (phosphate buffered saline) pH 7,4, Sigma-Aldrich®

Supplier: Merck

Washing buffer, PBS buffer solution (phosphate buffered saline) pH 7,4

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Buffer, TRIS solution pH 7 (1 mol/L), Trizma®, Sigma-Aldrich®

Supplier: Merck

Cloning Buffer, TRIS buffer solution pH 7 (1 mol/l)

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Buffer, TAE solution 10X concentrate (TRIS-acetate-EDTA buffer), Sigma-Aldrich®

Supplier: Merck

Running Buffer, TAE buffer solution 10X concentrate (TRIS-acetate-EDTA buffer)

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Buffer, TAE solution 10X concentrate (TRIS-acetate-EDTA buffer), Sigma-Aldrich®

Supplier: Merck

Running Buffer, TAE buffer solution 10X concentrate (TRIS-acetate-EDTA buffer)

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Buffer, TRIS-glycine solution 10X concentrate (TG buffer), Sigma-Aldrich®

Supplier: Merck

Electrophoresis, Transfer buffer, TRIS-glycine buffer solution 10X concentrate (TG buffer)

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Buffer, TRIS solution pH 7,5 (2 mol/L), Sigma-Aldrich®

Supplier: Merck

Cloning Buffer, TRIS buffer solution pH 7,5 (2 mol/l)

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Buffer, TBE solution 10X concentrate (TRIS-borate-EDTA buffer), Sigma-Aldrich®

Supplier: Merck

Running Buffer, TBE buffer solution 10X concentrate (TRIS-borate-EDTA buffer)

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Dulbecco's phosphate buffered saline (DPBS), cell culture, MP Biomedicals

Dulbecco's phosphate buffered saline (DPBS), cell culture, MP Biomedicals

Supplier: MP Biomedicals

Phosphate buffered salts, Dulbecco's formula (DPBS) is a balanced salt solution (BSS) used for the handling and culturing of mammalian cells. DPBS is used to to irrigate, wash, and dilute mammalian cells.

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ISE sample conditioning buffers and solutions

Supplier: WTW

Ion strength adjustment solutions have multiple tasks: TISAB for fluoride measurements does not only adjust the pH value, by also decomplexes the fluoride-containing complex compounds. Other ion strength adjustment solutions ensure an adapted pH value as well as the addition of a suitable conducting salt to correctly capture the concentration.

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