You searched for: Assays
An assay is an investigative procedure in laboratory medicine, pharmacology, environmental biology, and molecular biology for qualitatively assessing or quantitatively measuring the presence or functional activity of the analyte, or the entity being targeted by the assay. Generally, assays involve biological materials or phenomena which tend to be intrinsically more complex in composition or in behavior than what can be handled by traditional chemical analysis and titration.
Fluor-de-Lys® SIRT1 fluorometric drug discovery assay kit
Supplier: ENZO LIFE SCIENCES
A Fluor-de-Lys® fluorescent assay system. The SIRT1 Fluorescent Activity Assay/Drug Discovery Kit is a complete assay system designed to measure the lysyl deacetylase activity of the recombinant human SIRT1 included in the kit.
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LDH Cytotoxicity WST Assay
Supplier: ENZO LIFE SCIENCES
Colorimetric kit for the determination of cytotoxicity through the measurement of lactate dehydrogenase activity.
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ER-ID™ red assay kit (GFP-CERTIFIED®)
Supplier: ENZO LIFE SCIENCES
ER-ID® Red assay kit (GFP-CERTIFIED®) contains an endoplasmic reticulum-selective dye suitable for live cell, or detergent-permeabilized aldehyde-fixed cell staining.
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Calcium assay kits, FLIPR® Calcium 5 assay kit
Supplier: Molecular Devices
The FLIPR® Calcium 5 Assay Kit provides a fast, simple, and reliable fluorescence-based assay for detecting changes in intracellular calcium.
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Phosphatase assay kit, BIOMOL® Green
Supplier: ENZO LIFE SCIENCES
BIOMOL® Green provides a simple and convenient method for colorimetric phosphate quantitation. It is suitable for applications include assays for phospholipid phosphatases, tyrosyl-tRNA synthetase (coupled with pyrophosphatase) and a viral RNA triphosphatase. It is most widely applied to protein phosphatase assays.
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GOLGI ID® Green assay kit
Supplier: ENZO LIFE SCIENCES
The GOLGI ID® Green assay kit contains a Golgi apparatus-selective dye suitable for live cell, or aldehyde-fixed cell staining. It is more faithfully localized to the target organelle, with minimal staining of the endoplasmic reticulum. Micromolar concentrations of GOLGI ID® Green dye are sufficient for staining mammalian cells, as validated with human cervical carcinoma cell line HeLa, human T-lymphocyte cell line, Jurkat, canine kidney cell line MDCK, and human bone osteosarcoma epithelial cell line U2OS.
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QBT™ Fatty acid uptake assay kit
Supplier: Molecular Devices
QBT™ Fatty Acid Uptake Assay Kit is a homogeneous assay amenable to high-throughput screening. The kits deliver pre-optimized, fluorescence-based formulations to expedite assay development and screening of fatty acid transporters.
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Fluor-de-Lys® SIRT2 fluorometric drug discovery assay kit
Supplier: ENZO LIFE SCIENCES
A Fluor-de-Lys® fluorescent assay system. The SIRT2 Fluorescent Activity Assay/Drug Discovery Kit is a complete assay system designed to measure the lysyl deacetylase activity of the recombinant human SIRT2 included in the kit. The kit is ideal for chemical library screening for candidate inhibitors or activators or kinetic assay of the enzyme under varying conditions.
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Calcium assay kits, FLIPR® Calcium 6 and 6-QF assay kits
Supplier: Molecular Devices
The FLIPR® Calcium 6 and 6-QF Assay Kits provide a fast, simple, and reliable fluorescence based assay for detecting changes in intracellular calcium. The Calcium 6 Assay Kit significantly reduces fluorescence background with a one-step protocol. The Calcium 6-QF Assay Kit provides a quench-free option for sensitive targets or multiplexing applications.
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LDH Cytotoxicity Assay Kit
Supplier: Cayman Chemical
For measurement of LDH activity as an indicator of cell death.
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Granzyme B assay kit for drug discovery
Supplier: ENZO LIFE SCIENCES
The Granzyme B Assay Kit for Drug Discovery is a complete assay system designed to measure the protease activity of granzyme B. It contains a colorimetric substrate (IEPD-pNA) to measure this activity; cleavage of the p-nitroanilide (pNA) group from the colorimetric substrate increases absorption at 405 nm.
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Membrane potential assay kit, FLIPR®
Supplier: Molecular Devices
The FLIPR® Membrane Potential Assay Kit provide a fast, simple and reliable, fluorescence-based assay for detecting changes in voltage across the cell membrane and its homogenous fluorescence-based formulations for observation of real-time membrane potential changes associated with ion channel activation and ion transporter proteins.
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PROTEOSTAT® protein aggregation assay
Supplier: ENZO LIFE SCIENCES
PROTEOSTAT® Protein aggregation assay provides a simple, homogenous assay format for monitoring peptide and protein aggregation in solution. This is useful for defining optimal storage formulations for proteins, for screening of compounds that promote or inhibit protein aggregation and potentially for the sensitive measurement of molecular chaperone activity. The assay can be employed to streamline protein processing and formulation optimization procedures.
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LEADING LIGHT® Wnt Reporter Assay Starter Kit
Supplier: ENZO LIFE SCIENCES
The LEADING LIGHT® Wnt Reporter Assay Starter Kit is a cell-based luciferase activity test suitable for 96 well plate format.
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FLUOR DE LYS® SIRT3 fluorometric drug discovery assay kit
Supplier: ENZO LIFE SCIENCES
A Fluor-de-Lys® fluorescent assay system. The SIRT3 Fluorescent Activity Assay/Drug Discovery Kit is a complete assay system designed to measure the lysyl deacetylase activity of the recombinant human SIRT3 included in the kit. The kit is ideal for chemical library screening for candidate inhibitors or activators or kinetic assay of the enzyme under varying conditions.
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FLUOR DE LYS® HDAC1 fluorometric drug discovery assay kit
Supplier: ENZO LIFE SCIENCES
The HDAC1 Fluorescent Activity Assay/Drug Discovery Kit is a complete assay system designed to measure the lysyl deacetylase activity of the recombinant human HDAC1 included in the kit.
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Steady-Luc™ Firefly HTS assay kit
Supplier: Biotium
Firefly luciferase is widely used as a transcriptional reporter gene for research and drug screening. The Steady-Luc™ HTS kit can be used with luminescence plate readers without sample injection systems or for high-throughput screening.
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FLUOR DE LYS® HDAC fluorometric activity assay kit
Supplier: ENZO LIFE SCIENCES
The FLUOR DE LYS® HDAC fluorometric activity assay kit is a sensitive and convenient alternative to protocols utilizing radiolabeled, acetylated histones or peptide/HPLC methods for the assay of histone deacetylases. It is based on the unique FLUOR DE LYS® (Fluorimetric Histone deAcetylaseLysyl) substrate and developer combination and provides an assay that can be carried out in two simple mixing steps, all on the same 96-well plate. Histone deacetylase inhibitors have shown promise as anti-tumor agents and naturally this has stimulated interest in the screening of compounds for HDAC inhibition.
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Caspase-3 assay kits, NucView™ 488
Supplier: Biotium
NucView® 488 caspase-3 substrate is a novel cell membrane-permeable fluorogenic caspase substrate designed for detecting caspase-3 activity within live cells in real time.
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Cytochrome C releasing apoptosis assay kit
Supplier: ENZO LIFE SCIENCES
For the detection of Cytochrome c translocation from mitochondria into cytosol during apoptosis.
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eFluxx-ID® multidrug resistance assay kit
Supplier: ENZO LIFE SCIENCES
The EFLUXX-ID® Multidrug Resistance Assay Kit allows for functional detection of all three clinically relevant ABC transporter proteins MDR1 (p-glycoprotein), MRP1/2, and BCRP. The assay uses a hydrophobic, non-fluorescent compound that readily penetrates the cell membrane, where it is hydrolyzed to a hydrophilic fluorescent dye by intracellular esterases.
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FLUOR DE LYS®-Green HDAC2 fluorometric drug discovery assay kit
Supplier: ENZO LIFE SCIENCES
The HDAC2 Fluorescent Activity Assay/Drug Discovery Kit is a complete assay system designed to measure the lysyl deacetylase activity of the recombinant human HDAC2 included in the kit. The kit is ideal for chemical library screening for candidate inhibitors or kinetic assay of the enzyme under varying conditions.
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Protease Estimation Assay Kit, ProteSEEKER™
Supplier: G-Biosciences
ProteSEEKER™ identifies specific types of proteases with a panel of twelve protease inhibitors and a sensitive colorimetric protease screening assay. ProteSEEKER™ allows researchers to screen their protein samples and establish which specific class of proteases are present and therefore design a highly specific protease inhibitor cocktail using the minimal number of protease inhibitors. Alternatively, ProteSEEKER™ can be used to test existing protease inhibitor cocktails and identify their inadequacies and therefore supplement in additional protease inhibitors.
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Glycolysis Cell-Based Assay Kit
Supplier: Cayman Chemical
A colorimetric-based method for detecting L-lactate in culture medium.
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ProteoStat™ thioredoxin-1 assay kit for microplates
Supplier: ENZO LIFE SCIENCES
This kit provides a simple, homogenous assay for screening modulators of Thioredoxin-1 enzymatic activity.
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Caspase-1 assay kit for drug discovery
Supplier: ENZO LIFE SCIENCES
This kit is a complete assay system to measure protease activity of recombinant caspase-1. Cleavage of a tetrapeptide substrate is monitored colorimetrically at 405 nm or fluorimetrically (Ex. 360 nm/Em. 460 nm).
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APO-BRDU™ kit, TUNEL Assay
Supplier: Tonbo Biosciences
The APO-DIRECT™ reagent kit provides a two color TUNEL (Terminal deoxynucleotide transferase dUTP Nick End Labelling) assay for labeling DNA breaks and total cellular DNA to detect apoptotic cells by flow cytometry. The kit contains the instructions and reagents required for measuring apoptosis in cells including: positive and negative control cells for assessing reagent performance; washing, reaction and rinsing buffers for processing individual steps in the assay; terminal deoxynucleotidyl tranferase enzyme (TdT), fluorescein isothiocyanate (FITC) deoxyuridine triphosphate (FITC-dUTP) for labeling DNA breaks and PI/RNase A solution for counter staining the total DNA.
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Apoptosis assay kit, NucView® 488 and MitoView™ 633
Supplier: Biotium
NucView® 488 and MitoView™ 633 kit allows profiling of apoptotic cells simultaneously with changes in mitochondrial membrane potential using either fluorescence microscopy or flow cytometry.
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Caspase-2 fluorometric assay kit
Supplier: ENZO LIFE SCIENCES
Based on the detection of AFC (7-amino-4-trifluoromethyl coumarin) after cleavage from the labelled substrate VDVAD-AFC.
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Superoxide dismutase assay kit
Supplier: Cayman Chemical
Superoxide Dismutase Assay (SOD) kit is a fast and reliable assay for the measurement of SOD activity from plasma, serum, tissue homogenates and cell lysates.