2'-Fluoro NTPs are being utilised in an increasing number of applications in research and new drug development. 2'-Fluoro-dUTP is incorporated in both DNA and RNA constructs to improve in vivo stability.

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TriLink® Cytidine-5'-O-(1-Thiotriphosphate)

Supplier: TriLink BioTechnologies

TriLink® Cytidine-5'-O-(1-Thiotriphosphate)

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TriLink® N1-Ethylpseudouridine-5'-Triphosphate

Supplier: TriLink BioTechnologies

A modified NTP for incorporation into messenger RNAs (mRNA) using T7 RNA Polymerase. Incorporation of N1-ethylpseudouridine can reduce the immunogenicity of the resulting mRNA.

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TriLink® 2'-Fluoro-2'-deoxycytidine-5'-Triphosphate

Supplier: TriLink BioTechnologies

2'-Fluoro NTPs are being utilised in an increasing number of applications in research and new drug development. 2'-Fluoro-dCTP is incorporated in both DNA and RNA constructs to improve in vivo stability.

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TriLink® 2'-Deoxy-P-nucleoside-5'-Triphosphate

Supplier: TriLink BioTechnologies

2'-Deoxy-P-nucleoside is a degenerate base which behaves as a universal pyrimidine and therefore base pairs with both adenine and guanine. This feature makes it a powerful tool in mutagenesis studies.

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TriLink® N7-Methyl-3'-O-Methyl-Guanosine-5'-Triphosphate-5'-Guanosine

Supplier: TriLink BioTechnologies

TriLink® N7-Methyl-3'-O-Methyl-Guanosine-5'-Triphosphate-5'-Guanosine

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TriLink® 2'-Deoxyguanosine-5'-O-(1-Thiotriphosphate)

Supplier: TriLink BioTechnologies

TriLink® 2'-Deoxyguanosine-5'-O-(1-Thiotriphosphate)

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TriLink® 2'-Deoxythymidine-5'-O-(1-Thiotriphosphate)

Supplier: TriLink BioTechnologies

TriLink® 2'-Deoxythymidine-5'-O-(1-Thiotriphosphate)

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TriLink® 2'-Deoxyadenosine-5'-O-(1-Thiotriphosphate)

Supplier: TriLink BioTechnologies

TriLink® 2'-Deoxyadenosine-5'-O-(1-Thiotriphosphate)

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TriLink® 2'-Deoxycytidine-5'-O-(1-Thiotriphosphate)

Supplier: TriLink BioTechnologies

TriLink® 2'-Deoxycytidine-5'-O-(1-Thiotriphosphate)

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TriLink® Uridine-5'-O-(1-Thiotriphosphate)

Supplier: TriLink BioTechnologies

TriLink® Uridine-5'-O-(1-Thiotriphosphate)

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TriLink® Biotin-16-Aminoallyl-2'-deoxyuridine-5'-Triphosphate

Supplier: TriLink BioTechnologies

Biotin-16-Aminoallyl-2'-deoxyuridine can be enzymatically incorporated into DNA via PCR, nick translation, primer extension using Klenow(exo-) DNA polymerase, and terminal deoxynucleotidyl transferase (TdT). Biotinylated cDNA can be prepared with this NTP using Moloney Murine Leukemia Virus (M-MLV) reverse transcriptase.

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TriLink® 5-Propargylamino-2'-deoxyuridine-5'-Triphosphate

Supplier: TriLink BioTechnologies

5-Propargylamino-2'-deoxyuridine can be enzymatically incorporated into DNA and cDNA to introduce primary amine groups. Primary amines can be labeled with NHS ester fluors and haptens for detection and immobilisation of the resulting DNA strand.

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TriLink® Biotin-16-Aminoallyluridine-5'-Triphosphate

Supplier: TriLink BioTechnologies

Biotin-16-Aminoallyluridine-5'-Triphosphate (Biotin-16-UTP, Biotin-16-AA-UTP) can be incorporated via a number of RNA polymerases, including SP6-, T3- and T7-RNA polymerase to form labeled RNA.

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TriLink® Sulf Cyanine5 Aminoallyluridine-5'-Triphosphate

Supplier: TriLink BioTechnologies

Cyanine 5-Aminoallyluridine-5'-Triphosphate is a substrate for T7 RNA polymerase, allowing the incorporation of cyanine 5 into RNA and mRNA during in vitro transcription. Cyanine 5 incorporation allows for fluorescent detection of RNA and mRNA independent of protein translation.

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TriLink® 7-Deaza-2'-deoxyguanosine-5'-Triphosphate

Supplier: TriLink BioTechnologies

This deoxyguanosine analog lacks the N7 nitrogen found in the natural guanine nucleobase. Replacement of this nitrogen atom with a carbon atom results in diminished structure of the major groove in DNA and may disrupt base stacking, but does not interfere with Watson-Crick base pairing. This is desirable for applications such as amplification and sequencing of GC-rich nucleic acid sequences.

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TriLink® N6-Methyladenosine-5'-Triphosphate

Supplier: TriLink BioTechnologies

N6-methyl adenosine (N6-methyl ATP) is a base modified analog of adenosine and is found as a minor nucleoside in natural RNAs (Meyer et al.). N6-methyl ATP can substitute for ATP in some biological systems, and is a potent agonist for P2Y-purinoceptors in the guinea pig, taenia coli (Burnstock et al.).

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Lipid Removal Agent (LRA), Pore size: 90 Å, powder 325 mesh, Supelco®

Supplier: Merck

Lipid removal agent is a synthetic adsorbent of crystalline calcium silicate hydrate which is used for removing lipids and endotoxin from plasma or aqueous solutions. It is also used for DNA purification to remove protein, RNA and genomic DNA.

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VWR®, dNTPs

Supplier: VWR Chemicals

Ready to use molecular biology grade dNTP mixes and dNTP sets. The dNTP mix is designed to save hands-on time for researchers, and reduce the possibility of contamination by reducing pipetting. dNTP solutions are also available in sets of four individual dNTPs, each 100 mm. Both are convenient for use in DNA polymerisation reactions, DNA labelling and sequencing processes.

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