You searched for: Cell Culture Supplements and Additives
The tissue culture media supplements and additives compensate for characteristics the base medium lacks in providing maximum cell growth conditions. Incorporating the substances during development brings stability to the cultivated cell environments for maximum activity control. The stabilizing liquids added will support cell productivity, isolation, and microorganism identification. With a variety of tissue culture supplements and additives concentrations offered, a substance possessing the correct compatibility even with sensitive mediums is guaranteed.
Protease inhibitor cocktail, Recom ProteaseArrest™
Supplier: G-Biosciences
Recom ProteaseArrest™ is a broad range, bacterial, 100X concentrated, ready to use protease inhibitor cocktail. Recom ProteaseArrest™ offers greater protection for recombinant proteins expressed and purified from bacteria. Recom ProteaseArrest™ inhibits bacterial serine, cysteine, metallo- and other bacterial specific proteases, including aminopeptidases and aspartic proteases.
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Protease inhibitor cocktail, Plant ProteaseArrest™
Supplier: G-Biosciences
A broad range, 100X concentrated, ready-to-use protease inhibitor cocktail, Plant ProteaseArrest™ inhibits plant serine, cysteine and other plant specific proteases including aminopeptidases, aspartic and metalloproteases.
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HyClone Cell Boost™ 2 Supplement
Supplier: HyClone products (Cytiva)
Supplements cell culture with amino acids and glucose and manufactured to meet cGMP manufacturing standards and QC specifications.
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HyClone Cell Boost™ 1 supplement
Supplier: HyClone products (Cytiva)
Supplements cell culture with amino acids, vitamins, and glucose and manufactured to meet cGMP manufacturing standards and QC specifications.
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Trypsin
Supplier: Biowest
Trypsin is a porcine pancreas-derived enzyme that is commonly used for the dissociation and disaggregation of anchorage-dependent mammalian cells and tissues. The concentration of trypsin necessary to dislodge the cells from their substrate depends on the sensitivity of the cells.
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HyClone Cell Boost™ 5 supplement
Supplier: HyClone products (Cytiva)
Supplements cell culture with lipids, amino acids, vitamins, and growth factors. Manufactured to meet cGMP manufacturing standards and QC specifications.
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Protease inhibitor cocktails, ProteCEASE™
Supplier: G-Biosciences
ProteCEASE™ is a dry format version of ProteaseArrest™ for large scale preparative applications and for those users who prefer reconstitution prior to use. ProteCEASE™ is a superior general protease inhibitor cocktail that is suitable for purification from mammalian, plant, bacteria and yeast samples. The cocktail contains both irreversible and reversible protease inhibitors to inhibit serine, cysteine and other proteases. EDTA is an optional component for inhibiting metalloproteases.
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Trypsin Protease, HyClone™
Supplier: HyClone products (Cytiva)
Dissociation reagents, such as trypsin, are used to detach and disaggregate adherent cells from the surface or substrate to which they are attached, as well as for dissociating tissues for primary cell culture.
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HyClone Cell Boost™ 7a supplement
Supplier: HyClone products (Cytiva)
Supplements cell culture with amino acids, vitamins, salts, trace elements, and glucose. Manufactured to meet cGMP manufacturing standards and QC specifications.
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Phenol red, sodium salt
Supplier: Biowest
Phenol red is used as a pH indicator in cell culture. Its colour exhibits a gradual transition from yellow to red over the pH range 6,8 to 8,2. Above pH 8,2, phenol red turns a bright pink (fuchsia) colour.
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Protease inhibitor cocktail, Bacterial ProteaseArrest™
Supplier: G-Biosciences
A broad range, 100X concentrated, ready-to-use protease inhibitor cocktail, Bacterial ProteaseArrest™ inhibits bacterial serine, cysteine and other bacterial specific proteases, including aminopeptidases and aspartic proteases.
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Dispase II solution in PBS, Provitro
Supplier: PROVITRO
Immediately after delivery, place the passage kit in the dark at −20 °C. Prior use thaw the tube. After usage store them in the dark at 2 °C to 8 °C for a maximum of 2 weeks. Take care: After thawing shelf time of Dispase II solution is limited to 14 days.
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Ionotropic glutamatergic ligand library, Screen-Well®
Supplier: ENZO LIFE SCIENCES
The Screen-Well® ionotropic glutamatergic ligands plate contains 60 ligands including endogenous neurotransmitters, agonists, antagonists, and marketed drugs. The library is ideal for screening or identifying recombinant orphan G protein coupled receptors, target validation, secondary screening, assay development, and other pharmacological applications. The ionotropic glutamatergic ligands plate is 1 plate of the larger neurotransmitter library which contains over 680 ligands.
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Protease inhibitor set
Supplier: G-Biosciences
Protease inhibitor set contains 12 ready to use individual protease inhibitors, each at a 100X concentration. Various concentrations and/or combinations of protease inhibitors may be used to inhibit a broad spectrum of protease activity. The Protease inhibitor set can be used to design specific protease inhibitor cocktails, supplement existing cocktails or to screen for specific protease classes.
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Glutamine
Supplier: Biowest
L-glutamine is an essential amino acid required by virtually all mammalian and insect cells grown in culture. It is a crucial component of many cell culture media and serves as a major energy source for cells in culture. L-glutamine is very stable as a dry powder and as a frozen solution. However, in liquid media or stock solutions, L-glutamine can degrade relatively rapidly. L-glutamine is also more labile in cell culture solution than other amino acids.
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TM-235 defined serum-free growth medium
Supplier: PROTIDE PHARMACEUTICALS
Serum replacement with similar properties to TCM™ but contains additional components for cell types that have required higher concentrations of serum (typically more than 10%). TM-235™ is defined and multi-purpose. Contains highly purified, heat-treated bovine serum albumin, heat-treated bovine transferrin and recombinant insulin.
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Supplement Kit for MVEC Growth Medium, FCS
Supplier: PROVITRO
The supplement kit for microvascular endothelial cell growth medium is suitable for culturing Provitro human microvascular endothelial cells after adding the supplement kit components to 500 ml basal medium. The formulation is optimised for initial seeding of 4000 cells/cm² up to confluence (approx. 90%). Feeder-layer, matrix substrates or other substances are not necessary. Due to the possibility of reduced proliferative activity we recommend to use the antibiotic supplement for freshly isolated cells only.
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D-Glucose Monohydrate (Dextrose), cell culture tested
Supplier: Biowest
D-glucose monohydrate (Dextrose) is a common natural sugar involved in processes such as energy production, glycosylation, and formation of glycans that provide structure to cells. It is involved in a detrimental process in cells called glycation. It is used as a supplement for cell culture and in numerous cellular processes.
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Cryopreservation solution, DMSO-free, Cellvation™
Supplier: PROTIDE PHARMACEUTICALS
A unique cryopreservation solution that does not contain DMSO or foetal bovine serum. Cellvation™ is convenient to use and provides optimal recovery. Cells can be exposed to Cellvation™ for longer periods of time before the freezing process is initiated. Cellvation™ is ready to use and can be used for cells cultured in serum-free medium or with serum-containing medium.
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Supplement Kit for Skeletal Muscle Cell Growth Medium, FCS
Supplier: PROVITRO
The Provitro skeletal muscle cell growth medium is a sterile liquid culture medium for culturing human skeletal muscle cells (HSKMC). The medium is delivered as a basal medium and is suitable for culturing Provitro HSKMC after adding the supplement kit components. The formulation is optimised for initial seeding of 4000 cells/cm² up to confluence (approx. 90%). Feeder-layer, matrix substrates or other substances are not necessary. Due to the possibility of reduced proliferative activity we recommend to use the antibiotic supplement for freshly isolated cells only.
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Cell detachment solution, Accutase
Supplier: Biowest
Accustase is a ready to use cell detachment solution of proteolytic and collagenolytic enzymes. Accutase is a direct gentle replacement for trypsin solution. It can also be used on suspension cells to reduce their agglutination in order to ease counting.
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Protease inhibitor cocktails, ProteaseArrest™
Supplier: G-Biosciences
The ProteaseArrest™ family of protease inhibitor cocktails are supplied as convenient, ready-to-use, liquid format cocktails that offer immediate >95% inhibition of harmful proteases. The 100X concentrated solution outperforms other similar protease inhibitor cocktails (including tablet formats) by offering instant inhibition, allowing delivery of optimised concentrations, demonstrating higher inhibition levels, and providing a greater ease of use. Also available as single use OneQuant™ ProteaseArrest™ aliquots.
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TCM defined serum-free growth medium
Supplier: PROTIDE PHARMACEUTICALS
General serum replacement for many cell types from a variety of species. TCM™ is a defined, multi-purpose serum replacement useful in studying the effects of growth factors and the production of proteins. TCM™ contains highly purified, heat-treated bovine serum albumin, heat-treated bovine transferrin and recombinant insulin.
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Protease inhibitor cocktail, Yeast/Fungal ProteaseArrest™
Supplier: G-Biosciences
A broad range, 100X concentrated, ready-to-use protease inhibitor cocktail, Yeast/ Fungal ProteaseArrest™ inhibits yeast and fungal serine, cysteine and metalloproteases.
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FDA approved drug library V2, Screen-Well®
Supplier: ENZO LIFE SCIENCES
Accelerates drug optimisation with a unique collection of compounds of known safety and bioavailability for diverse targets.
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Opioid ligand library, Screen-Well®
Supplier: ENZO LIFE SCIENCES
The Screen-Well® opioid and sigma ligands plate contains 74 ligands including endogenous neurotransmitters, agonists, antagonists, and marketed drugs. The library is ideal for screening or identifying recombinant orphan G protein coupled receptors, target validation, secondary screening, assay development, and other pharmacological applications. The opioid and sigma ligands plate is 1 plate of the larger Neurotransmitter Library which contains over 650 ligands.
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GABAergic ligand library, Screen-Well®
Supplier: ENZO LIFE SCIENCES
The GABAergic igands library contains 56 ligands including endogenous neurotransmitters, agonists, antagonists, and marketed drugs. The library is ideal for screening or identifying recombinant orphan G protein-coupled receptors, target validation, secondary screening, assay development, and other pharmacological applications. The GABAergics ligands plate is 1 plate of the larger neurotransmitter library which contains over 650 CNS receptor ligands.
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Metabotropic glutamatergic ligand library, Screen-Well®
Supplier: ENZO LIFE SCIENCES
The Screen-Well® metabotropic glutamatergic ligands plate contains 49 ligands including endogenous neurotransmitters, agonists, antagonists, and marketed drugs. The library is ideal for screening or identifying recombinant orphan G protein coupled receptors, target validation, secondary screening, assay development, and other pharmacological applications. The metabotropic glutamatergic ligands plate is 1 plate of the larger neurotransmitter library which contains over 650 ligands.
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Hematopoietic library, Screen-Well®
Supplier: ENZO LIFE SCIENCES
The Screen-Well® hematopoietic toxicity library is a focused collection of 115 compounds with defined and diverse hematopoietic toxicity, including myelosuppression, neutropenia, leukopenia, anaemia, and many more. A variety of structurally and mechanistically different compound classes are included, as well as nontoxic controls. The compounds are dissolved in DMSO at 10 mM and aliquoted into deep-well plates. The library is an essential tool for predictive toxicology screening and assay development.