You searched for: Enzymes
Enzymes accelerate, or catalyze, chemical reactions, and they are known to catalyze more than 5,000 biochemical reaction types. Most enzymes are proteins, although a few are catalytic RNA molecules. Choose specific enzymes for cleaving bonds, removing genomic DNA from RNA preparations, for producing fragments of proteins, or for use in ion exchange chromatography. Enzymes are used in the chemical industry and other industrial applications when extremely specific catalysts are required.
Proteinase K solution
Supplier: PanReac AppliChem
Proteinase K is used to destruct proteins in cell lysates (tissue, cultured cells) and to liberate nucleic acids, since it very effectively digests DNases and RNases.
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Aspergillus niger Glucose oxidase
Supplier: Apollo Scientific
Lyophilized powder. From Aspergillus niger. Specific activity: IU/mg protein>300 units. IU per 1mg powder is approximately 450 units.
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Leuconostoc mesenteroides Sucrose phosphorylase
Supplier: Apollo Scientific
Lyophilized powder. From Leuconostoc mesenteroides. Specific activity: IU/mg protein>100 units. IU per 1mg powder is approximately 100 units.
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Peroxidase, Horseradish, RZ3 (POD, HRP, HRPO)
Supplier: US Biological
Horseradish Peroxidase (HRP) is a hemoprotein catalysing the oxidation by hydrogen peroxide of a number of substrates such as ascorbate, ferrocyanide, cytochrome C and the leuko form of many dyes.
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Deoxyribonuclease I
Supplier: Rockland Immunochemicals
Deoxyribonuclease I (usually called DNase I), is an endonuclease coded by the human gene DNASE1. DNase I is a nuclease that cleaves DNA preferentially at phosphodiester linkages adjacent to a pyrimidine nucleotide, yielding 5'-phosphate-terminated polynucleotides with a free hydroxyl group on position 3', on average producing tetranucleotides. It acts on single-stranded DNA, double-stranded DNA, and chromatin.
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Human Recombinant 15-Lipoxygenase-2 (from E. coli)
Supplier: Cayman Chemical
Two types of 15-lipoxygenase (15-LO) have been discovered and characterised, both of which metabolise arachidonic acid (AA) to produce 15(S)-hydroxyeicosatetraenoic acid (15(S)-HETE). 15-LO-1 oxygenates AA at both C-15 and C-12, whereas 15-LO-2 exclusively oxygenates C-15 of AA. Human 15-LO-2 has a molecular mass of approximately 76 kDa and exhibits approximately 40% identity to the reticulocyte 15-LO-1.2,3 expression of 15-LO-2 appears to be restricted to prostate, lung, skin, and cornea and may play a role in the normal development of these tissues.
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Salmonella minnesota R595 (Re) Lipid A
Supplier: Enzo Life Sciences
Isolated from Salmonella minnesota R595 (Re mutant). Prepared by mild acid hydrolysis from purified Salmonella minnesota R595 (Re) LPS (isolated by a modification of the PCP extraction method), converted to the uniform salt form and dissolved in sterile pyrogen-free double distilled water.
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Klenow (3'-5' exo-) (from E. coli carrying the Klenow (3'-5' exo-) gene)
Supplier: Enzo Life Sciences
Klenow (3′-5′ exo-) is a mesophilic DNA polymerase deficient in both proofreading (3′-5′) and nick-translation (5′-3′) nuclease activities, and that displays a moderate strand displacement activity during DNA synthesis. The protein is expressed as a truncated product of the E.coli PolA gene and contains the D355A and E357A mutations.
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Bovine MSG-Trypsin™
Supplier: G-Biosciences
Trypsin is a serine endopeptidase that specifically cleaves peptide bonds on the carboxy side of s-aminoethyl cysteine, arginine and lysine residues and typically there is little or no cleavage at arginyl-proline and lysyl-proline bonds. The distribution of these residues in proteins allows trypsin digestion to produce peptides that are readily identified by mass spectrometry.
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Human Recombinant EN-TEV Protease (from E. coli)
Supplier: Abcam
Recombinant EN-TEV Protease protein is a Tobacco etch virus Fragment protein, in the 2038 to 2279 aa range, expressed in Escherichia coli, with >=95% purity and suitable for SDS-PAGE, Size Exclusion Chromatography.
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Nuclease S1
Supplier: Rockland Immunochemicals
Nuclease S1 is an endonuclease that is active against single-stranded DNA and RNA molecules. Although its primary substrate is single-stranded, it can also introduce single-stranded breaks in double-stranded DNA-RNA or DNA-RNA hybrids. Nuclease S1 is used in the laboratory as a reagent in nuclease protection assays. It is used in removing single stranded tails from DNA molecules to create blunt ended molecules and opening hairpin loops generated during synthesis of double stranded cDNA.
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Yeast recombinant thioreduxin reductase (NAPH)
Supplier: Apollo Scientific
Yeast recombinant thioreduxin reductase (NAPH)
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Human recombinant Matrix Metalloproteinase-13
Supplier: Apollo Scientific
Human recombinant Matrix Metalloproteinase-13
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Human recombinant Adenosine Deaminase tRNA-Specific 1
Supplier: Apollo Scientific
Human recombinant Adenosine Deaminase tRNA-Specific 1
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Chicken lysozyme (Muramidase) (from egg white)
Supplier: Rockland Immunochemicals
Chicken lysozyme (Muramidase) (from egg white)
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Human recombinant peptidyl-Prolyl Cis/Trans isomerase
Supplier: Apollo Scientific
Human recombinant peptidyl-Prolyl Cis/Trans isomerase
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Cholera Toxin (Vibrio cholerae) G Protein Activator
Supplier: Enzo Life Sciences
G protein activator.
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D-Glucose dehydrogenase
Supplier: Thermo Fisher Scientific
Applications: Diagnostics and cofactor regeneration of NAD(P)H in reductive processes.
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Human recombinant Matriptase
Supplier: Enzo Life Sciences
Recombinant human matriptase (aa 596-855) fused at the N-terminus to a His-tag.
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Human recombinant Guanylyl cyclase alpha1beta1 (soluble) (from Sf9 cells)
Supplier: Enzo Life Sciences
Produced in Sf9 cells. Fused at the C-terminus to a His-tag.
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Human recombinant Matriptase 2
Supplier: Enzo Life Sciences
Produced in Drosophila Schneider 2 (S2) cells. Recombinant human matriptase-2 (aa 78-811) is fused at the C-terminus to a V5-His-tag.
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Mg²⁺/ATP Activating Solution
Supplier: Enzo Life Sciences
This solution contains pre-coupled Mg-ATP for immediate use as an energy source for a range of ATP-dependent reactions (e.g. ubiquitin thioester conjugation, proteasomal degradation, SUMO conjugation).
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Human Recombinant Aldehyde Dehydrogenase 1-A2 (from E. coli)
Supplier: ProSci Inc.
Aldehyde dehydrogenase 1 family member A2 (ALDH1A2), also known as retinaldehyde dehydrogenase 2 (RALDH2), belongs to the aldehyde dehydrogenase family which contains two members, the ALDH1 s (ALDH1A1, ALDH1A2 and ALDH1A3) and the 9-cis retinaldehyde dehydrogenase ALDH8 s.
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T4 GP32 Protein (from E.coli)
Supplier: G-Biosciences
T4 GP32 is a single-stranded DNA binding protein with key role in DNA replication, recombination and repair. T4 GP32 binds cooperatively to single stranded DNA and stimulates cell-free DNA synthesis directed by the T4-encoded DNA polymerase. It improves the efficiency of reverse transcriptase during RT-PCR as well as increases the yield of PCR products.
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Dnase
Supplier: OMEGA BIO-TEK
For DNase digestion during RNA purification. The RNase-free DNase set provides a rapid and efficient on-column digestion of DNA during RNA purification from a wide variety of sample sources using E.Z.N.A.® RNA kits. The DNases can be efficiently removed in subsequent steps. Generally, DNase digestion is not required for RNA purified with E.Z.N.A.® RNA kits since the silica-membrance, spin-column technology efficiently removes the majority of the DNA without DNase treatment. However, complete DNA removal may be necessary for certain RNA applications that are sensitive to very small amounts of DNA.