Transactivation response (TAR) DNA-binding protein of 43 kDa (also known as TARDBP or TDP-43) was first isolated as a transcriptional inactivator binding to the TAR DNA element of the HIV-1 virus. Neumann et al. (2006) found that a hyperphosphorylated, ubiquitinated, and cleaved form of TARDBP, known as pathologic TDP-43, is the major component of the tau-negative and ubiquitin-positive inclusions that characterise amyotrophic lateral sclerosis (ALS) and the most common pathological subtype of frontotemporal lobar degeneration (FTLD-U). 60019-2-Ig is a mouse monoclonal antibody recognizing the cleavage product of 20-30 kDa in addition to the native and phosphorylated forms of TDP-43. Immunohistochemical analyses of TDP-43 using this antibody detect both normal diffuse nuclear staining and insoluble inclusions in pathologic tissues. The epitope of 60019-2-Ig has been determined to locate at residues 203-209 by Hiroshi Tsuji et al. (2012), which is involved in the formation of pathologic TDP-43 aggregation. Notably this antibody only recognizes human TDP-43 but not reacts with mouse or rat TDP-43.

Western Blot:K-562 Cells, 1:5000-1:50000; IF: MCF-7 Cells, 1:10-1:100; IP:K-562 Cells, 1:500-1:5000; FC: MCF-7 Cells, N/A; IHC: Human Brain(FTLD-U) Tissue, N/A

Type: Primary
Antigen: TDP43
Clonality: Monoclonal
Clone: 6H6E12
Conjugation: Unconjugated
Epitope:
Host: Mouse
Isotype: IgG1
Reactivity: Human