Human Histamine ELISA kit is a competitive Enzyme-Linked Immunosorbent Assay (cELISA) designed for the in vitro quantitative determination of human Histamine in serum, plasma, tissue homogenates, and other biological fluids.

• Ready To Use ELISA kit
• Detection Range: 1,563 to 100 ng/ml
• Sensitivity: 0,938 ng/ml
• Sample Type: Serum, plasma, tissue homogenates and other biological fluids
• Assay Precision: Intra - Assay: CV <8%, Inter - Assay: CV <10%

Human Histamine ELISA kit (A79810) employs the competitive enzyme immunoassay technique for the quantitative measurement of human Histamine in serum, plasma, tissue homogenates, and other biological fluids. The 96-well microtiter plate has been pre-coated with Histamine antigen. During the incubation, Histamine present in the samples or standards competes with the fixed amount of immobilized Histamine for binding sites on the Biotinylated Anti-Histamine Antibody. The more Histamine present in a sample or standard, the less Biotinylated Anti-Histamine Antibody that binds to the plate. Following incubation, unbound Biotinylated Anti-Histamine Antibody is removed by washing, and an HRP-Streptavidin conjugate is added to the wells and the microtiter plate is incubated. Following incubation and washing, TMB substrate solution is then used to visualize the HRP enzymatic reaction by catalysis to produce a blue-coloured product that changes to yellow after addition of acidic stop solution. The density of yellow is inversely proportional to the amount of Histamine present in each sample or standard. The concentration of Histamine can then be calculated by reading the O.D. absorbance at 450 nm in a microplate reader and referring to the standard curve.