Specifications
- Pk:96 Tests
- Assay duration:Multiple steps
- Assay Type:Competitive
- Format:Pre-coated
- Primary antibody reactivity:Other
- Target protein:Arachidonic Acid
- Description:Arachidonic acid ELISA kit
- Sample type:Serum, plasma, tissue homogenates and other biological fluids
- Cross reactivity:Arachidonic Acid ELISA kit exhibits high specificity and excellent specificity for the detection of universal Arachidonic Acid. No significant cross-reactivity or interference between Arachidonic Acid and analogues was observed.
- Detection method:Colorimetric
- Time to Results:4 h 30 min
- Assay Principle:Quantitative
- Shelf life:Store for 6 months at 4 °C
- Detection range:1,563 - 100 µg/ml
- Storage temperature:4 °C
- Sample volume:50 μl
- Sensitivity:0,938 µg/ml
- Regulatory status:RUO
Specifications
About this item
Arachidonic Acid ELISA kit is a competitive Enzyme-Linked Immunosorbent Assay (cELISA) designed for the in vitro quantitative determination of Arachidonic Acid in serum, plasma, tissue homogenates, and other biological fluids.
- Ready-to-use ELISA kit
- Detection range: 1,563 to 100 µg/ml
- Sensitivity: 0,938 µg/ml
- Sample type: Serum, plasma, tissue homogenates, and other biological fluids
- Assay Precision: Intra-Assay: CV <8%, Inter-Assay: CV <10%
Arachidonic Acid ELISA kit (A74629) employs the competitive enzyme immunoassay technique for the quantitative measurement of universal Arachidonic Acid in serum, plasma, tissue homogenates, and other biological fluids. The 96-well microtiter plate has been pre-coated with Arachidonic Acid antigen. During the incubation, Arachidonic Acid present in the samples or standards competes with the fixed amount of immobilised Arachidonic Acid for binding sites on the Biotinylated Anti-Arachidonic Acid Antibody. The more Arachidonic Acid present in a sample or standard, the less Biotinylated Anti-Arachidonic Acid Antibody that binds to the plate. Following incubation, unbound Biotinylated Anti-Arachidonic Acid Antibody is removed by washing, and an HRP-Streptavidin conjugate is added to the wells and the microtiter plate is incubated. Following incubation and washing, TMB substrate solution is then used to visualise the HRP enzymatic reaction by catalysis to produce a blue-coloured product that changes to yellow after addition of acidic stop solution. The density of yellow is inversely proportional to the amount of Arachidonic Acid present in each sample or standard. The concentration of Arachidonic Acid can then be calculated by reading the O.D. absorbance at 450 nm in a microplate reader and referring to the standard curve.