Mouse Ghrelin ELISA kit is a competitive Enzyme-Linked Immunosorbent Assay (cELISA) designed for the in vitro quantitative determination of mouse Ghrelin in serum, plasma, tissue homogenates, and other biological fluids.

• Ready to use ELISA kit
• Detection range: 3,125 to 200 pg/ml
• Sensitivity: 1,875 pg/ml
• Sample type: Serum, plasma, tissue homogenates, and other biological fluids
• Assay precision: Intra assay: CV <8%, Inter assay: CV <10%

Mouse Ghrelin ELISA kit (A303536) employs the competitive enzyme immunoassay technique for the quantitative measurement of mouse Ghrelin in serum, plasma, tissue homogenates, and other biological fluids. The 96-well microtiter plate has been pre-coated with Ghrelin antigen. During the incubation, Ghrelin present in the samples or standards competes with the fixed amount of immobilised Ghrelin for binding sites on the Biotinylated Anti-Ghrelin antibody. The more Ghrelin present in a sample or standard, the less Biotinylated Anti-Ghrelin antibody that binds to the plate. Following incubation, unbound Biotinylated Anti-Ghrelin antibody is removed by washing, and an HRP-Streptavidin conjugate is added to the wells and the microtiter plate is incubated. Following incubation and washing, TMB substrate solution is then used to visualise the HRP enzymatic reaction by catalysis to produce a blue-coloured product that changes to yellow after addition of acidic stop solution. The density of yellow is inversely proportional to the amount of Ghrelin present in each sample or standard. The concentration of Ghrelin can then be calculated by reading the O.D. absorbance at 450 nm in a microplate reader and referring to the standard curve.