buffers

Supplier: G-Biosciences

FirstChoice™ Blocking Buffer is ideal as a first choice for optimization of new assays and systems, or when determining the optimal blocking buffer for elimination of non specific binding sites in ELISA, blotting, immunohistochemistry and other applications. Buffers are conveniently supplied in widely used TBS (Tris-buffered saline at pH 7,5) and PBS (phosphate-buffered saline at pH 7,5) buffers as well as in separate formulations containing Tween® 20 for improving blocking efficiencies.

Supplier: THERMO FISHER DIAGNOSTICS

Cell culture buffer, Phosphate buffered saline tablets

Supplier: G-Biosciences

Gram-negative lysis buffer is an extraction buffer for soluble proteins from Gram-negative bacteria. It is a proprietary improvement on the lysozyme based lysis in combination with various salts and agents, which allows extraction of soluble proteins. This buffer has been tested for use with several widely used bacteria including E.coli strains.

Supplier: Apollo Scientific

Cloning Buffer, TE buffer (1X)

Supplier: Abcam

ChIP Buffer for ab185913

Supplier: VWR Chemicals

4X concentrated buffer solution (1,5 M Tris, pH 8,8 and 0,4% SDS). Used for preparation of standard acrylamide:bis-acrylamide gels as well as preparation of gels using PAGE-PLUS™ acrylamide solution.

Supplier: Biotium

Ready-to-use formaldehyde-based fixation buffer for immunofluorescence staining for microscopy or flow cytometry.

Supplier: Enzo Life Sciences

Caspase cell lysis buffer, Enzo Life Sciences

Supplier: G-Biosciences

The hybridisation buffers like Church & Gilbert’s, Denhardt solution, hybridisation denaturing solution, hybridisation neutralising solution, SSC, SSPE, TMAC Hyb solution and TNT Buffer are used in wide range of applications. Church & Gilbert’s Hybridization Buffer is prepared from molecular biology grade SDS (sodium dodecyl sulfate), monosodium phosphate (NaHPO₄), EDTA (ethylenediaminetetracetic acid) and bovine serum albumin (BSA) Fraction V using DNase, RNase and Protease-free water. It consists of 1mM EDTA, 1% BSA, 0,5M sodium phosphate monobasic, and 7% SDS (at a pH of 7,2).

Supplier: VWR Chemicals

Washing buffer, PBS buffer solution 10X concentrate (phosphate buffered saline)

Supplier: VWR Chemicals

Wash buffers ideal for use in applications such as Western blotting and ELISA. Prevents high background by effectively removing unbound reagents.

Supplier: VWR Chemicals

Phosphate Buffered Saline (PBS) with 0,05% Tween® 20, pH 7,5 is a ready-to-use 1× concentration.

Supplier: G-Biosciences

Premixed buffer powders that simply require adding deionised water to generate a ready-to-use buffer.

Supplier: VWR Chemicals

Rapid transfer buffer is a simple, single component system for quick and efficient transfer of proteins from SDS-PAGE gels to membranes for Western blotting applications. Transfer is completed in 10 or 20 minutes using a standard semi-dry or wet transfer apparatus, respectively.

Supplier: Thermo Fisher Scientific

Lithium Nitrate Buffer Solution

Supplier: Thermo Fisher Scientific

Cesium Nitrate Buffer solution