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703 results for "buffers"

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Buffers

Resisting pH changes, buffers protect samples when acids or alkali is added. Powder and liquid forms efficiently track proteins during electrophoresis isolation and separation. The salty buffers can also be used to calibrate and standardize pH meters for accurate readings. Blocking substances stop antibody attachment to membrane free sites that would produce an interfering background. In various concentrations to fit any need, the buffers may come in liquids or dehydrated formats.

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20X SSPE, liquid, Corning®

20X SSPE, liquid, Corning®

Supplier: Corning

20X Saline-Sodium-Phosphate-EDTA (SSPE) buffer has applications in nucleic acid hybridization and other molecular biology work.

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Proteometer-UFT Fluorescent Reagent

Proteometer-UFT Fluorescent Reagent

Supplier: NOVILYTIC

Set of three reagent vials for use with the Proteometer-UFT kit.

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Proteometer-CV Starter Kit Reagent (60)

Proteometer-CV Starter Kit Reagent (60)

Supplier: NOVILYTIC

Fluorescent reagent portion of the Proteometer-CV starter kit. This is sold individually due to shipping and storage conditions.

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pH buffer solutions, referenced at 20 °C, Fluka™

Supplier: Honeywell Chemicals

pH Buffer solution, Glycine, pH value: 13,00, ±0.05, Reference temperature: 20 °C, Fluka™, Plastic bottle, 6×1 L

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Buffer solutions, colour coded, Fluka™

Supplier: Honeywell Chemicals

These buffer solutions are colour-coded for easy identification and the pH 4 and 7 version have fungicide additive.

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HEPES Buffer, ES Qualified, HyClone™ AdvanceSTEM™

HEPES Buffer, ES Qualified, HyClone™ AdvanceSTEM™

Supplier: HyClone products (Cytiva)

HyClone AdvanceSTEM ES Qualified HEPES provides maintenance of physiological pH in cell culture despite changes in carbon dioxide.

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Proteometer-CV Full Kit Reagent (300)

Proteometer-CV Full Kit Reagent (300)

Supplier: NOVILYTIC

Fluorescent reagent portion of the Proteometer-CV full kit. This is sold individually due to shipping and storage conditions.

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Proteometer-CV Reconstitution Reagent

Proteometer-CV Reconstitution Reagent

Supplier: NOVILYTIC

Proteometer-CV reconstitution reagent.

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Buffer, imidazole buffer solution pH 7,5 (2 mol/L)

Supplier: G-Biosciences

Imidazole buffer solution pH 7,5 (2 mol/l)

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His buffer kit

His buffer kit

Supplier: Cytiva

His Buffer Kit contains carefully prepared buffer concentrates for binding, washing, and elution of histidine-tagged proteins according to recommended protocols. Moreover, a method for optimization of imidazole concentration in the binding buffer is described.

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Proteometer-L Fluorescent Reagent

Proteometer-L Fluorescent Reagent

Supplier: NOVILYTIC

Set of 7 reagent vials for use with the Proteometer-L kit.

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pH buffer solutions, referenced at 20 °C, traceable to SRM from NIST, Fluka™

Supplier: Honeywell Chemicals

pH Buffer solution, Citrate, pH value: 2,00, ±0.02, Reference temperature: 20 °C, Fluka™, 6×1 L

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Proteometer-L Starter Kit Reagent (200)

Proteometer-L Starter Kit Reagent (200)

Supplier: NOVILYTIC

Fluorescent reagent for the Proteometer-L Starter Kit. This is sold separately due to shipping conditions.

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Tris Borate EDTA buffer, for molecular biology, 10x, DNAse, RNAse and Protease free, pH 8.3

Supplier: Thermo Fisher Scientific

Running Buffer, TBE buffer solution 10X concentrate (TRIS-borate-EDTA buffer)

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Buffer solution for chlorine determination

Buffer solution for chlorine determination

Supplier: Hach

For determination of chlorine by amperometric titration. APHA formulation 100 ml bottle.

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EDTA solution, 0,5 M, molecular biology grade, Calbiochem®, Millipore®

Supplier: Merck Millipore (Calbiochem‎)

Sterile-filtered solution of 0,5 M EDTA in H₂O treated with diethyl pyrocarbonate (DEPC). Used in molecular biology applications.

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PBS, 10X solution, molecular biology grade, ultrapure

Supplier: Thermo Fisher Scientific

Washing buffer, PBS, 10X solution, molecular biology grade, ultrapure

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2-D Protein Extraction Buffer

2-D Protein Extraction Buffer

Supplier: Cytiva

Efficient cell lysis and protein extraction are key steps for achieving high-quality results in downstream applications. The 2-D protein extraction buffer trial kit is designed for those who experiment for establishing a suitable extraction and solubilization buffer for their protein samples.

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Blocking reagents, Blotto

Blocking reagents, Blotto

Supplier: Rockland Immunochemicals

Contains immunoanalytical grade non-fat dry milk. Blocks non specific binding of proteins in various immunological assays. Ideal for use in western blotting and ELISA methods.

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Ab buffer kit

Ab buffer kit

Supplier: Cytiva

Ab buffer kit contains prepared buffer concentrates for binding, washing, and elution of IgG according to recommended protocols.

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SSC 20X buffer, MP Biomedicals

Supplier: MP Biomedicals

Role of a balanced salt solution in cell culture is multifaceted and can be divided into four principal functions:

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CSPL buffer

Supplier: OMEGA BIO-TEK

Component of the Mag-Bind® plant DNA 96 plus kit.

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Sodium bicarbonate, Corning®

Sodium bicarbonate, Corning®

Supplier: Corning

Sodium bicarbonate is used as part of a buffering system commonly used to maintain physiological pH 7,2 to 7,4 in a culture environment

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Protein A/G HP SpinTrap™ Buffer Kit

Protein A/G HP SpinTrap™ Buffer Kit

Supplier: Cytiva

Protein A/G HP SpinTrap™ Buffer kit is optimised for standard affinity-based enrichment/immunoprecipitation according to the protocol provided with protein A HP SpinTrap™, protein G HP SpinTrap™, and Ab SpinTrap™ columns.

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GTE buffer

Supplier: Thermo Fisher Scientific

Thermo Fisher Scientific

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TBS with Tween® (TBST), 20X solution, Molecular Biology Grade, ultrapure

Supplier: Thermo Fisher Scientific

TBS is used in Western blotting protocols.

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Solubilisation buffer, 2D-Xtract™

Solubilisation buffer, 2D-Xtract™

Supplier: G-Biosciences

A protein solubilisation buffer for 2D analysis must solubilise proteins effectively, without disturbing the native charge of the proteins. Urea based solubilisation buffers solubilise proteins effectively, however can modify the native charge of the proteins, due to carbamylation. Urea exists in equilibrium with ammonium cyanate that modifies α- and ε-amino groups, inducing changes in the isoelectric point of proteins leading to artifactual results.

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Tris-HCl, 1M solution, pH 8,0, Molecular Biology Grade, Ultrapure

Supplier: Thermo Fisher Scientific

1M solution of Tris, Ultrapure in high purity distilled H₂O, adjusted to pH 8,0.

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Y-PER™ plus Yeast Protein Extraction Reagent, Dialysable

Y-PER™ plus Yeast Protein Extraction Reagent, Dialysable

Supplier: Thermo Fisher Scientific

This extraction reagent is formulated to effectively lyse yeast and bacterial cells to extract and solubilise proteins for analysis.

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HEPES Buffer, 1 M Sterile, Cell Culture Grade, MP Biomedicals

HEPES Buffer, 1 M Sterile, Cell Culture Grade, MP Biomedicals

Supplier: MP Biomedicals

HEPES (N-2-hydroxyethylpiperazine-N-2-ethane sulfonic acid) is a general purpose zwitterionic organic chemical buffering agent which does not bind magnesium, calcium, manganese(II) or copper (II) ions.

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