35347 Results for: "MP Biomedicals"

Supplier: MP Biomedicals

Each impact-resistant 2 ml tube contains 1,4 mm ceramic spheres, 0,1 mm silica spheres, and one 4 mm glass bead. Lysing Matrix E tubes have purple caps and found in the FastDNA™ SPIN Kit for Soil and the FastRNA™ Pro Soil Kits. Lysing Matrix E can also be effective in lysing mixed samples, such as microbe infected tissue, and can be used in RNA extraction from tumours and other difficult tissues.

Supplier: MP Biomedicals

Lysing Matrix D is a critical component of FastPrep® sample preparation system. Each impact-resistant 2 ml tube contains 1,4 mm ceramic spheres. Lysing Matrix D tubes have green caps and are found in the FastDNA™ Spin Kit for Plant and Animal Tissues and FastRNA™ Pro Green Kit for isolation of nucleic acids from plants and animals.

Supplier: MP Biomedicals

The Fast DNA™ SPIN Kit for Soil is designed to efficiently isolate bacterial, fungal, plant and animal genomic DNA from soil and other environmental samples. It quickly and efficiently isolates PCR-ready genomic DNA directly from soil samples in less than 60 minutes.

Supplier: MP Biomedicals

β-Mercaptoethylamine (Cysteamine hydrochloride) is useful antioxidant. It is experimentally used as a radioprotective agent. It is also used to produce acute and chronic duodenal ulcers in rats and as an antidote to acetaminophen.

Supplier: MP Biomedicals

Applications
Guanidine hydrochloride can be used as the first step in refolding proteins or enzymes into their active form. Urea and dithiothreitol (DTT) may also be necessary. Also used in the isolation of RNA. It is a strong chaotropic agent useful for the denaturation and subsequent refolding of protein, it can solubilize insoluble or denatured proteins such as inclusion bodies and be used for the recovery of periplasmic proteins. This can be used as the first step in refolding proteins or enzymes into their active form. Urea and dithiothreitol (DTT) may also be necessary. Also used in the isolation of RNA.
Product Description
The crystal structure of Guanidine hydrochloride consists of a network of guanidinium cations and chloride anions linked by N–H•••Cl hydrogen bonds,it is a strong chaotropic agent.Guanidine HCl may agglomerate upon storage. It may appear as a free-flowing crystalline powder, a freeflowing powder with solid material dispersed throughout, or a solid. The quality of the product does not appear to be affected and solutions prepared from the free-flowing and lumpy guanidine HCl appear identical

Grade: Ultra Pure
Purity: >99%
Keywords: Guanidium chloride, chaotropic agent
Key Applications: Chaeotropic agent
Product Type: Biochemicals
Biochemical Category: Chaotropic Agents
Density: 1.345 g/cm³ at 20 °C (Lit.)
Melting Point: 180-190 °C
UV/Visible Absorbance: OD260nm (6.0 M aq soln) <0.03
Presentation: White Crystalline Powder
pH: 4-6 (6.0 M aq soln)
Solubility: Soluble in water,Clear, Colorless Solution (6 M - clear, colorless solution).
Storage & Handling: Room Temperature, desiccate

Supplier: MP Biomedicals

Iodoacetamide is a thiol reagent; alkylating reagent for cysteine and histidine residues in proteins. In the alkylation reaction, it reacts with histidine (such as in RNase), methionine and sulfhydryl groups of many proteins.

Supplier: MP Biomedicals

Eosin Y disodium salt is used in combination with Azure II to show morphological details of marrow cells; buffered Azure A/Eosin Y method for demostrating cell granules, microorganisms and nuclei; for showing insect musculature; for demonstrating cells infected with mosquito-iridescent virus; use in combination with Alum Hematoxylin/Azure II to show mast cells, early necrobiotic changes in cells and protozoans, rickettsias and bacteria in tissues; to demonstrate volutin granules in any microorganism; Bond's modification of Mann's Eosin-Methyl Blue for Negri bodies in smears; Harris stain for Negri bodies in smears; Mann's method for staining Negri bodies in sections; Gridley's stain for Endamoeba in tissue sections; a modified methenamine-silver nitrate method for the demonstration of Endamoeba in sections. It is used as a counterstain for hematoxylin.

Supplier: MP Biomedicals

Storage: Store at -20 °C.
Brefeldin A is a fungal metabolite which is a macrocyclic lactone exhibiting a wide range of antibiotic activity. Produced by Penicillium brefeldianum. Blocks binding of the cytosolic coat protein b-COP and ARF to Golgi membranes mediated by protein G. Also blocks protein transportation into post-Golgi compartments. It activates the sphingomyelin cycle. Brefeldin A mediated apoptosis has been observed in human tumor cells.
Brefeldin A reversibly inhibits the intracellular translocation of proteins in eukaryotes, e.g., during transport of proteins to the cell surface for secretion or expression. It has been reported to block the response of cultured cells to cholera toxin. In HepG2 cells, BFA induces two blocks in the secretory pathway; one at the level of the endoplasmic reticulum-Golgi juncture and the other in the trans-Golgi network. Brefeldin A is used in the studies of Brefeldin A-inhibited Guanine Nucleotide-exchange Protein, BIG2, Regulates the Constitutive Release of TNFR1 Exosome-like Vesicles.
Brefeldin A (BFA) is a fungal metabolite which disrupts the structure and function of the Golgi apparatus. BFA is an activator of the sphingomyelin cycle. Brefeldin A-mediated apoptosis has been observed in human tumor cells.

Supplier: MP Biomedicals

Superoxide dismutases are enzymes that catalyse the dismutation of superoxide into oxygen and hydrogen peroxide. They are an important antioxidant defense in nearly all cells exposed to oxygen.

Supplier: MP Biomedicals

Storage: Store at room Temperature (15-30 °C)
Sucrose is a nonreducing disaccharide composed of glucose and fructose, linked via their anomeric carbons. It is obtained commercially from Sugarcane, sugar beet (Beta vulgaris), and other plants and used extensively as a food and a sweetener.
Use to create sucrose gradients for purification of viruses and proteins.

Supplier: MP Biomedicals

Storage: -20°C, desiccate
This is an ultrapure NAD, chromatographically purified to remove trace inhibitors.
β-NAD, a pyridine nucleotide and biologically active form of nicotinic acid, is a coenzyme necessary for the catalytic reaction of certain enzymes. It occurs in living cells primarily in the oxidized state. Serves as a coenzyme of the dehydrogenases, especially in the dehydrogenation of primary and secondary alcohols. NAD usually acts as a hydrogen acceptor, forming NADH which then serves as a hydrogen donor in the respiratory chain.
Many metabolites and enzymes of biological interest are present in tissues at low concentrations. With the use of β-NAD as a catalyst intermediate and several enzymes in a multistep system, known as enzyme cycling, much greater sensitivity for detection of these components is achieved. The reduced form, β-NADH, is fluorescent whereas β-NAD is not. This difference in fluorescence provides a sensitive fluorescent measurement of the oxidized or reduced pyridine nucleotides at concentrations down to 10-7 M.
Electron acceptor. β-NAD is a carrier for hydride ion, forming b-NADH. Hydride ion is enzymatically removed from a substrate molecule by the action of dehydrogenases such as malic dehydrogenase and lactic dehydrogenase. Such enzymes catalyze the reversible transfer of a hydride ion from malate or lactate to b-NAD to form the reduced product, b-NADH. Unlike b-NAD which has no absorbance at 340 nm, b-NADH absorbs at 340 nm (EmM = 6.22). The increase in absorbance at 340 nm with the formation of b-NADH is the basis for measurement of activity of many enzymes.

Supplier: MP Biomedicals

Polyvinylpyrrolidone is a water-soluble polymer made from the monomer N-vinylpyrrolidone. Typically used as thickeners, dispersing agents, detoxicant, complexing agent, lubricants and binders.

Supplier: MP Biomedicals

p-Nitroblue Tetrazolium Chloride is a substrate for dehydrogenases and other oxidases and precipitating agent for fibrinogen.

Supplier: MP Biomedicals

Alizarin red S is used in a biochemical assay to determine, quantitatively by colorimetry, the presence of calcific deposition by cells of an osteogenic lineage.

Supplier: MP Biomedicals

It is useful for structural and biological studies, as a reference standard, or as starting material for immunogen preparation. It is prepared using salt fractionation or by chromatographic techniques.

Supplier: MP Biomedicals

Specific Surface Area: 200 m2/g
Particle Size: 63-200 µm
Weight: 0.8 g/ml pH: 10

Supplier: MP Biomedicals

SDS is an anionic detergent and wetting agent that is effective in both acid and alkaline solutions.

Supplier: MP Biomedicals

Soluble in water (100 mg/ml, swells and dissolves slowly); insoluble in alcohol.

Supplier: MP Biomedicals

Protamine sulphate is a purified mixture of simple protein principles obtained from the sperm or testes of suitable species of fish, which has the property of neutralizing heparin. Because of having much basic amino acids (mostly arginine) protamine contains far greater nitrogen than other proteins. Its molecular weight is relatively small. Histone and other basic proteins in the testes of unmatured fishes convert into protamine along with the growth of the fishes. In the testes protamine takes the form of nucleoprotamine linked with DNA.

Supplier: MP Biomedicals

HYDROCORTISONE-21-ACETATE (Δ4-Pregnen-11β,17α,21-triol- 3,20-dione; Cortisol acetate) Crystalline

Supplier: MP Biomedicals

γ-L-Glutamyl-p-Nitroanilide hydrochloride is suitable for use as a substrate for g-Glutamyl transpeptidase.

Supplier: MP Biomedicals

L-threonine is classified as an essential amino acid. Synthesised from homoserine phosphate by threonine synthase or from acetaldehyde and glycine by serine hydroxy methyltransferase.

Supplier: MP Biomedicals

Poly-lysine is a polycation which binds to DNA, red cell membrane and any negatively charged protein. When adsorbed to the culture surface, poly-lysine increases the number of positively charged sites available for cell binding. A compromise between the easier to use lower molecular weight products and the extremely viscous higher molecular weights would be the products in the range of 70,000-150,000.
It is typically used as a coating substrate for culture dishes, slides, etc. It enhances electrostatic interaction between negatively charged ions of the cell membrane and the culture surface. Both the D- and L- form of the poly-lysine can be used as a coating substrate since poly-lysine is a nonspecific attachment factor for cells; however, certain cells can digest poly-lysine. In this case, poly-D-lysine should be used as the attachment factor so that the cells are not disrupted by excessive uptake of L-lysine.
Other uses for poly-lysine have been reported as well:
• Conjugation to methotrexate for increased drug transport.
• Microencapsulation of islets.
• Use in simple reproducible procedure for chromosomal preparations from a variety of tissues.
• Immobilation of living cells in biocompatible semipermeable microcapsules.
• In the preparation of polycationic beads.
• Conjugation to albumin and horseradish peroxidase to enhance cellular uptake.
Poly-D-lysine hydrobromide is a synthetic amino acid that enhances cell adhesion to solid substrates. It has additionally been shown to eliminate prion proteins from infected cells.

Supplier: MP Biomedicals

Vincristine Sulfate, a dimeric Vinca alkaloid. Experiments have reported Vincristine Sulfate to demonstrate a capacity to suppress growth of proliferating cells through marked apoptosis. The cell death caused by this agent seems to result in a sustained accumulation of endogenous ceramide levels. Ceramide, has been proposed as a lipid second messenger with specific antiproliferative mediating responses.

Supplier: MP Biomedicals

Vinblastine Sulphate is an anti-tumor alkaloid.

Supplier: MP Biomedicals

-Aminosalicylic acid is an analogue of p-aminobenzoic acid. It is an antitubercular agent. The sodium salt of the drug is better tolerated than the free acid.

Supplier: MP Biomedicals

Adenosine 5'-monophosphate disodium salt (AMP disodium salt) hydrate ≥99%, powder

Supplier: MP Biomedicals

The methylated xanthine compounds caffeine, theobromine, and theophylline and their derivatives are used in medicine for their bronchodilator effects.

Supplier: MP Biomedicals

Polymyxin B sulphate is a mixture of Polymyxin B1 and Polymyxin B2 sulphate, obtained from Bacillus polymyxia strains. Polymyxin B inhibits gram-negative organisms in vitro wherein it binds to and interferes with the permeability of the bacterial cytoplasmic membrane and is also reported to inhibit Ca²⁺ dependent protein kinase C (PKC). Effective concentration is typically 50 mg/l.

Supplier: MP Biomedicals

Tannic Acid is used for enzyme immobilization and protein adsorption