Supplier: G-Biosciences

Immobilised β-cyclodextrin is designed to allow refolding of recombinant proteins that were aggregated or formed inclusion bodies when expressed in the genetically engineered cells. The refolding of misfolded proteins is adapted from GroE chaperone refolding mechanism.

Supplier: G-Biosciences

Bsu DNA Polymerase. Large fragment is from Bacillus subtilis and is modified to retain its 5'→ 3' DNA polymerase activity with its 5'→ 3' exonuclease domain removed. It also lacks the 3'→ 5' exonuclease activity.

Supplier: G-Biosciences

T4 recombination mediator protein, UvsY plays important roles in homologous recombination (HR). During HR, the UvsX recombinase competes with the prebound single-stranded binding protein gp32 for DNA-binding sites and UvsY stimulates this filament nucleation event.

Supplier: G-Biosciences

Nonidet® P 40 Substitute (NP-40) 10% in aqueous solution

Supplier: G-Biosciences

N-Decyl-N,N-dimethyl-3-ammonio-1-propanesulphonate(sulphobetaine-10, SB-10)

Supplier: G-Biosciences

TRITON™ X-100 (Polyethylene glycol tert-octylphenyl ether)

Supplier: G-Biosciences

NDSB 201 is a zwitterionic compound. Unlike zwitterionic detergents, the hydrophobic group in NDSB 201 is too short to form micelles, even at 1 M concentrations. NDSB 201 can be used for the purification of proteins and solubilisation of protein samples for 2D gel electrophoresis.

Supplier: G-Biosciences

Sodium deoxycholate

Supplier: G-Biosciences

GenLysates™ are total protein tissue and cell lysates that are extracted from the tissue or cell of interest. To inhibit proteolysis, the GenLysates™ are prepared in a lysis buffer containing a complete protease inhibitor cocktail. Each lysate lot is tested by electrophoresis and Western blot analysis.

Supplier: G-Biosciences

ProteCEASE™ is a dry format version of ProteaseArrest™ for large scale preparative applications and for those users who prefer reconstitution prior to use. ProteCEASE™ is a superior general protease inhibitor cocktail that is suitable for purification from mammalian, plant, bacteria and yeast samples. The cocktail contains both irreversible and reversible protease inhibitors to inhibit serine, cysteine and other proteases. EDTA is an optional component for inhibiting metalloproteases.

Supplier: G-Biosciences

Adenine deaminase converts adenine to hypoxanthine. It is a highly purified recombinant enzyme protein supplied at a 1 µM concentration.

Supplier: G-Biosciences

Running Buffer, Tris-Glycine native gel running buffer

Supplier: G-Biosciences

Anti-IgG Goat Antibody (AP (Alkaline Phosphatase))

Supplier: G-Biosciences

Anti-IgG Goat Antibody (HRP (Horseradish Peroxidase))

Supplier: G-Biosciences

(Bis(2-[Succinimidooxycarbonyloxy]ethyl) sulfone Homobifunctional Cross Linkers.

Supplier: G-Biosciences

Anti-IgG Goat Antibody (HRP (Horseradish Peroxidase))

Supplier: G-Biosciences

The FOCUS™ Reduction-Alkylation Kit offers a simple two-step method for reduction and alkylation of protein samples for 2D gel analysis.

Supplier: G-Biosciences

Add an appropriate volume of the supplied rehydration buffer to the dry, ready-to-use, urea based equilibration buffer.

Supplier: G-Biosciences

FOCUS™ SubCell for the fractionation of mitochondrial, nuclear, and cytosolic soluble and membrane proteins.

Supplier: G-Biosciences

InGel™ provides a reliable method for the proteolytic digestion and subsequent extration of silver stained proteins in gel for subsequent analysis by mass spectrometry.

Supplier: G-Biosciences

HOOK™ NHS-LC-LC-Biotin, 50 mg

Supplier: G-Biosciences

Protein-S-S-Reductant™ uses TCEP (Tris [2-carboxyethyl] phosphine), a popular alternative to β-mercaptoethanol and DTT (dithiothreitol). TCEP improves stability, increases effectiveness, and reduces proteins over a wider range of pH, including lower acidic pHs. Protein-S-S-Reductant™ completely reduces stable disulfide bonds in less than 5 minutes at room temperature and is compatible with the protein alkylation reactions. This ready to use solution is at a neutral pH and stabilised for long-term storage. Simply supplement Protein-S-S-Reductant™ in place of DTT or β-mercaptoethanol and boil the sample.

Supplier: G-Biosciences

E-64 is an irreversible inhibitor of cysteine proteases. It interacts with the Sn subsites of proteases but has no action on cysteine residues in other proteins.

Supplier: G-Biosciences

Pepstatin is a potent inhibitor of various aspartic proteases, including cathepsin D, renin, pepsin, bacterial aspartic proteases and HIV proteases. Soluble in DMSO or methanol.

Supplier: G-Biosciences

The HOOK™ Peptide Coupling kits allow researchers to attach peptides to a carrier protein of choice using either primary amines or sulfhydryls in their peptide. The HOOK™ Peptide Coupling kits can be supplied with or without carrier proteins.

Supplier: G-Biosciences

There are many electrophoresis running buffers including: TAE running buffer (50×), TBE running buffer (10×), TBE-urea sample buffer, MOPS running buffer (10×), and MOPS/ SDS running buffer (20×). These include the SDS-PAGE sample loading buffer, SDS-PAGE running buffer, native sample loading buffer, tris glycine native gel running buffer, tricine sample buffer, tris-tricine, tris-tricine-SDS, MES SDS running buffer, MOPS/ SDS running buffer, IEF anode buffer, IEF cathode buffer, zymogram sample buffer, zymogram renature buffer, zymogram development buffer and the discontinuous buffer system.

Supplier: G-Biosciences

SG-Glutamic-C™ is a serine endopeptidase, from S. aureus V8, that is highly specific for the cleavage of peptide bonds at the carboxy side of either aspartic or glutamic acid, depending on the buffer used. In Tris-HCl buffer, in particular in the absence of phosphate ions, the enzyme is specific for the glutamyl site. Recommended buffers for fragmentation of proteins using this enzyme are 50 mM Tris-HCI, pH 8,0 or bicarbonate buffer. Highly purified preparations of SG-Glutamic-C™ are chemically modified making the enzyme both resistant to autolysis and stabilises its enzymatic activity.

Supplier: G-Biosciences

Ideal for enzyme-antibody crosslinking.

Supplier: G-Biosciences

The Nuclear and Cytoplasmic Extraction kit is for the enrichment of cytoplasmic and nuclear fractions from cultured cells and tissues. The kit generates a cleaner separation of cytoplasmic proteins from nuclear proteins and is ideal for expression and transport studies.

Supplier: G-Biosciences

Potassium acetate 3 mol/l (3 N) in aqueous solution