28451 Results for: "G-Biosciences"

Supplier: G-Biosciences

Iodoacetamide

Supplier: G-Biosciences

Ethidium bromide

Supplier: G-Biosciences

GET™ CLEAN DNA uses high binding affinity GET™ spin columns to remove salts, enzymes, unincorporated nucleotides, radiolabels, and primer-dimers from any DNA preparation of 100 bp to 20 kb. GET™ spin columns has an enhanced binding affinity for DNA, thus eliminating loss or damage of DNA.

Supplier: G-Biosciences

Protein-OUT™ is a washing solution that cleans plastic and glassware used in the proteolytic digestion and peptide isolation for mass spectrometry analysis. The rinsing of plastic and glassware with Protein-OUT™ will remove interfering chemicals and peptides that can leach from the tube walls and interfere or suppress the mass spectrometry signal. In addition, rinsing with Protein-OUT™ removes inadvertent contamination with dust and keratin.

Supplier: G-Biosciences

Swift™ Film Cleaner allows researchers to clean film that has been overexposed or has a high background or speckling without having to repeat experiments. Suitable for all exposed film developed for gel shift assays, Western, Southern and Northern blots.

Supplier: G-Biosciences

Triton® X-100 is for solubilising membrane proteins during isolation of membrane-protein complexes.

Supplier: G-Biosciences

Specifically designed for bacterial research and supplied with bacteria specific reagents. Extracts and solubilises nearly all of the proteins from E.coli, including membrane as well as soluble proteins. Extraction is based on the gentle lysis of bacterial cells with LongLife™ Lysozyme enzyme, followed by extraction of total proteome with the supplied strong chaotropic extraction buffer that solubilises even the most difficult proteins.

Supplier: G-Biosciences

Dithiothreitol (DTT, Cleland's reagent) 2 mol/l in aqueous solution

Supplier: G-Biosciences

Total Protein Extraction (TPE™) Kit is a universal lysis system for the solubilisation of total proteins from animal, plant, yeast, bacteria, and other biological samples. The kit contains ready to use buffers for extracting total protein from cells and tissues for SDS-PAGE analysis.

Supplier: G-Biosciences

GenLysates™ are total protein tissue and cell lysates that are extracted from the tissue or cell of interest. To inhibit proteolysis, the GenLysates™ are prepared in a lysis buffer containing a complete protease inhibitor cocktail. Each lysate lot is tested by electrophoresis and Western blot analysis.

Supplier: G-Biosciences

A DNase and RNase free solution of 0.5M EDTA.

Supplier: G-Biosciences

Tris is an abbreviation of the organic compound known as tris(hydroxymethyl)aminomethane, with the formula (HOCH2)3CNH2. Tris is extensively used in as a major component in buffer solutions.

Supplier: G-Biosciences

Chymostatin inhibits serine proteases having a chymotrypsin-like specificity, including α, β γ, and δ chymotrypsin, and most cysteine proteases including cathepsins B, H, L. Soluble in DMSO.

Supplier: G-Biosciences

FOCUS™ Mitochondria kit is specifically designed for the isolation of intact mitochondria from cultured mammalian cells. This kit allows for the fast and efficient fractionation of the cytoplasm of cultured mammalian cells into an enriched fraction of mitochondria. The majority (>90%) of the mitochondria have intact inner and outer membranes and therefore retain their functionality.

Supplier: G-Biosciences

Leupeptin, hemisulphate inhibits serine, plasmin, porcine kallikrein and cysteine proteases, including papain and cathepsin B. Does not inhibit chymotrypsin and thrombin

Supplier: G-Biosciences

Dialysis tubes, Tube-O-DIALYZER™ centrifuge tube adapter

Supplier: G-Biosciences

Genomic Tube‐O‐DIALYZER™ (0.45µm). 

Supplier: G-Biosciences

Big CHAP (N,N-Bis[3-(D-gluconamido)propyl]cholamide)

Supplier: G-Biosciences

Zwitterionic detergent, Aminosulfobetaine with C14 alkyl tail is useful for solubilizing proteins for 2D analysis. Optimal solubility achieved in urea-thiourea mixtures and not in urea alone.

Supplier: G-Biosciences

(3-((3-Cholamidopropyl)dimethylammonio)-2-hydroxy-1-propanesulphonate)

Supplier: G-Biosciences

3-[(3-Cholamidopropyl)dimethylammonio]-1-propane sulphate (CHAPS)

Supplier: G-Biosciences

Recombinant human caspases 1 - 3 and 6 - 10 are supplied lyophilised in quantities of 25 units. One unit of the specific recombinant caspase is the enzyme activity that cleaves one nanomole of a specific caspase substrate per hour at 37 °C in a reaction solution containing 50mM HEPES (at a pH of 7,2), 50 mM NaCl, 0,1% CHAPS, 10 mM EDTA, 5% glycerol and 10 mM DTT.

Supplier: G-Biosciences

3-[(3-Cholamidopropyl)dimethylammonio]-1-propane sulphate (CHAPS)

Supplier: G-Biosciences

GenLysates™ are total protein tissue and cell lysates that are extracted from the tissue or cell of interest. To inhibit proteolysis, the GenLysates™ are prepared in a lysis buffer containing a complete protease inhibitor cocktail. Each lysate lot is tested by electrophoresis and Western blot analysis.

Supplier: G-Biosciences

CytoScan™ WST-1 Cell Proliferation Assay principle is based upon the reduction of the tetrazolium salt WST-1 to formazan by cellular dehydrogenases. The generation of the dark yellow coloured formazan is measured at 420 to 480 nm (optimal at 440 nm) and is directly correlated to cell number.

Supplier: G-Biosciences

GenLysates™ are total protein tissue and cell lysates that are extracted from the tissue or cell of interest. To inhibit proteolysis, the GenLysates™ are prepared in a lysis buffer containing a complete protease inhibitor cocktail. Each lysate lot is tested by electrophoresis and Western blot analysis.

Supplier: G-Biosciences

A broad range, 100X concentrated, ready-to-use protease inhibitor cocktail, Mammalian ProteaseArrest™ inhibits mammalian serine, cysteine and other mammalian specific proteases including aminopeptidases, trypsin-like and aspartic proteases.

Supplier: G-Biosciences

Sodium dodecyl sulphate (SDS)

Supplier: G-Biosciences

GET™ Plasmid DNA Miniprep Kit isolates high quality plasmid DNA from 1 to 5 ml E. coli cultures. The kit utilises an enhanced DNA binding column to produce high yields of plasmid.

Supplier: G-Biosciences

GenLysates™ are total protein tissue and cell lysates that are extracted from the tissue or cell of interest. To inhibit proteolysis, the GenLysates™ are prepared in a lysis buffer containing a complete protease inhibitor cocktail. Each lysate lot is tested by electrophoresis and Western blot analysis.