1449 Results for: "Cytiva"

Supplier: Cytiva

PD SpinTrap G-25 is a single-use column for cleanup of large biomolecules.

Supplier: Cytiva

Superdex prep grade (pg) is produced by covalent bonding of dextran to highly cross-linked agarose.The separation properties of these media are determined by the dextran component

Supplier: Cytiva

Protein G Sepharose 4 Fast Flow has recombinant protein G immobilized by the cyanogen bromide (CNBr) method to Sepharose 4 Fast Flow.

Supplier: Cytiva

TempliPhi™ Kits efficiently prepare micrograms of circular DNA from picogram input material. The DNA templates are prepared by rolling circle amplification (RCA) using bacteriophage Phi29 DNA polymerase. TempliPhi™ uses an isothermal method for the exponential amplification of circular DNA. Phi29 DNA polymerase is active at 30 °C, enabling amplification to be performed at this temperature without the need for thermal cycling. The TempliPhi™ protocol requires less than 20 minutes of hands-on time to amplify 96 samples from bacterial colonies.

Supplier: Cytiva

HiTrap TALON® crude 1 and 5 ml columns allow for rapid separations and easy scale-up with a minimum of sample preparation and equipment.

Supplier: Cytiva

PD MiniTrap G-25 columns are prepacked, single-use gravity columns for desalting, buffer exchange, and sample cleanup.

Supplier: Cytiva

NHS-activated Sepharose™ 4 Fast Flow is composed of cross-linked 4% agarose beads, NHS activated for coupling amino-containing proteins and peptides in process-scale applications. NHS (N-hydroxysuccinimide) coupling forms a chemically stable amide bond with ligands containing primary amino groups. The resin provides a spacer arm to the coupled ligand and suitable for immobilising smaller protein and peptide ligands.

Supplier: Cytiva

Amersham™ Protran® membranes are manufactured from 100% pure nitrocellulose to ensure the highest binding capacity possible. They are compatible with a variety of detection methods, including isotopic, chemiluminescent, colorimetric and fluorescent. No methanol pre-wetting step is required, making this the membrane of choice for proteins which prefer aqueous environments. Prior to transfer the membrane is simply wetted with water, and then placed in the transfer buffer. No other pretreatment steps are necessary.

Supplier: Cytiva

CNBr-activated Sepharose™ 4B is pre-activated media used for coupling antibodies or other large proteins containing -NH₂ groups to the Sepharose™ media, by the cyanogen bromide method, without an intermediate spacer arm.

Supplier: Cytiva

Rainbow™ molecular weight markers enable faster and simpler identification of proteins on SDS polyacrylamide gels. These ready to load markers provide sharper, more intense bands on gels and blots, and have improved band spacing for more accurate molecular weight determination. The bright, distinctive colours of the markers allow easier confirmation of transfer to blotting membranes and orientation.

Supplier: Cytiva

Flow restrictor, FR-902

Supplier: Cytiva

Superdex prep grade (pg) is produced by covalent bonding of dextran to highly cross-linked agarose.The separation properties of these media are determined by the dextran component

Supplier: Cytiva

Highly species specific HRP-conjugated antibodies optimized for use with Amersham ECL Western Blotting Detection Reagents.

Supplier: Cytiva

For rapid buffer exchange/desalting of PCR products and other DNAs in a volume of 10 to 100 μl using spin column chromatography.

Supplier: Cytiva

PuReTaq RTG PCR beads and reagents ensures reliable and robust performance in both end point and RT-PCR amplifications and ensures the lowest possible levels of contaminating prokaryotic and eukaryotic nucleic acids.

Supplier: Cytiva

Thrombin is a protease used to digest fusion proteins prepared from pGEX vectors containing the recognition sequence for thrombin (pGEX-1lT, pGEX-2T, pGEX-2TK, pGEX-4T-1, pGEX-4T-2, and pGEX-4T-3).

Supplier: Cytiva

Superose 6 Increase SEC columns are designed for high-resolution, small-scale preparative purification, and analysis of large globular proteins, protein complexes, and other macromolecules in the molecular weight range 5 to 5000 kD.

Supplier: Cytiva

HisTrap excel columns are prepacked with Ni Sepharose excel affinity resins for capture and purification of histidine-tagged proteins secreted into eukaryotic cell culture supernatants by immobilized metal ion affinity chromatography (IMAC).

Supplier: Cytiva

Amersham™ Hybond™ P 0,45 PVDF is a 0,45 μm pore size hydrophobic membrane, with high physical strength, highly suitable for stripping and reprobing. For use in chemiluminescent and fluorescent detection methods for proteins of >Mr 20000.

Supplier: Cytiva

Ficoll-Paque™ PREMIUM products are a range of sterile, ready to use density gradient media for the preparation of mononuclear cells.

Supplier: Cytiva

HiTrap NHS-activated HP prepacked chromatography columns allow you to design an affinity column suitable for your purification needs.

Supplier: Cytiva

NAP™ columns are disposable columns prepacked with Sephadex™ G-25 DNA grade and require only gravity to run.

Supplier: Cytiva

HisTrap™ FF columns are designed for simple, one-step purification and scaling up purification of histidine-tagged proteins. The columns are pre-packed with Ni Sepharose™ 6 Fast Flow.

Supplier: Cytiva

Ni Sepharose 6 Fast Flow is a BioProcess medium that combines the advantages of Ni₂₊ for purification of histidine tagged proteins, with the well industrially established properties of the Sepharose Fast Flow platform.

Supplier: Cytiva

illustra™ ExoProStar™ 1-STEP Kit uses illustra™ Exonuclease I and Alkaline Phosphatase for optimal removal of unincorporated primers and nucleotides. illustra™ ExoProStar™ improves digestion efficiency with no degradation of the target PCR product. The sample remains completely intact and ready for immediate use in manual or automated processes.

Supplier: Cytiva

ProbeQuant™ G-50 micro columns use spin column chromatography to provide a fast and reliable method for purifying labelled probes from unincorporated labelled nucleotides. In addition, a portion of the eluted DNA can be counted in a scintillation counter and compared to an unpurified sample to give an approximation of percent incorporation. The columns are designed for use in a microcentrifuge, come prepacked with Sephadex™ G-50 DNA grade and are pre-equilibrated in STE, which means that they are ready to use. They can accommodate samples ranging in volume from 25 μl to 50 μl.

Supplier: Cytiva

Highly species specific HRP-conjugated antibodies optimized for use with Amersham ECL Western Blotting Detection Reagents.

Supplier: Cytiva

Sera-Mag Carboxylate-Modified Magnetic Beads & SpeedBeads have free carboxyl groups on the surface for convenient covalent coupling of target molecules (proteins, peptides, and amine-modified ligands) via primary amines (NH₂).

Supplier: Cytiva

Percoll® is commonly used for separating cells, organelles, viruses, and other subcellular particles.

Supplier: Cytiva

Amersham™ ECL™ Prime is a highly sensitive Western blotting detection reagent characterised by stable signal emission, allowing for the possibility of repeated exposures and making it easier to process several blots in the same experimental run. Amersham™ ECL™ Prime delivers a detection system that is sensitive, stable, precisely quantitative across a wide dynamic range of protein levels, and conservative in its consumption of expensive antibody reagents. The high performance of Amersham™ ECL™ Prime is due to the presence of an enhancer in the reagent, increasing enzyme turnover, and thereby significantly increasing both signal intensity and duration. The light signal is further intensified by the addition of a catalyst.