Rat Thrombospondin 2 ELISA kit is a sandwich Enzyme-Linked Immunosorbent Assay (sELISA) designed for the in vitro quantitative determination of rat Thrombospondin 2 in serum, plasma or other biological fluids.

Rat Thrombospondin 2 ELISA Kit (A4976) employs the sandwich enzyme immunoassay technique for the quantitative measurement of rat Thrombospondin 2 in serum, plasma or other biological fluids. An antibody specific for Thrombospondin 2 has been pre-coated onto a 96-well microtiter plate. The standards and test samples are added into the wells and the Thrombospondin 2 present in each sample is bound to the wells by the immobilised antibody. Following incubation, the wells are washed and then incubated with Biotinylated Anti-Thrombospondin 2 Antibody, which binds the captured Thrombospondin 2 present in each well. Following incubation, unbound biotinylated detection antibody is removed by washing, and an HRP-Avidin conjugate is added to the wells and the microtiter plate is incubated. Following incubation and washing, TMB substrate solution is then used to visualise the HRP enzymatic reaction by catalysis to produce a blue-coloured product that changes to yellow after addition of acidic stop solution. The density of yellow is proportional to the amount of Thrombospondin 2 captured in each well. The concentration of Thrombospondin 2 can then be calculated by reading the O.D. absorbance at 450 nm in a microplate reader and referring to the standard curve.